NTH1 Antibody

Images

 
Western Blot: NTH1 Antibody [NB100-108] - Detection of NTH1 (37 kDa) from Hela & K562 cell extracts using NB100-108 (1:500). WB data courtesy of Mark Kelly, Indiana Univ.
Immunocytochemistry/ Immunofluorescence: NTH1 Antibody [NB100-108] - NTH1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human colon adenocarcinoma using NTH1 antibody at 5ug/ml concentration (1:200 ...read more
Western Blot: NTH1 Antibody [NB100-108] - Analysis of NTH1 expression in 1) HeLa, 2) A-431, 3) MCF7 whole cell lysates.
Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lymph node cancer using 5 ug/ml concentration of NTH1 antibody. The representative ...read more
Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human kidney cancer using 5 ug/ml concentration of NTH1 antibody. The renal cancer cells ...read more
Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal cancer using 5 ug/ml concentration of NTH1 antibody. The rectal cancer cells as ...read more
Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal cancer using 5 ug/ml concentration of NTH1 antibody. This representative image ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.1 mg/ml

NTH1 Antibody Summary

Immunogen
A peptide derived from human NTH1, conjugated to KLH. [UniProt# P78549]
Predicted Species
Bovine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NTHL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:800
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This NTH1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a single band is detected at 37 kDa representing NTH1. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
K-562 Whole Cell Lysate
HeLa Whole Cell Lysate
Publications
Read Publications using
NB100-108 in the following applications:

  • IHC
    2 publications
  • 2 publications
  • WB
    2 publications

Reactivity Notes

Human, mouse and rat. Immunogen sequence has 93% identity with bovine proteins.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NTH1 Antibody

  • EC 4.2.99.18
  • endonuclease III-like protein 1
  • nth endonuclease III-like 1 (E. coli)
  • NTH1
  • NTHL1
  • OCTS3
  • OCTS3NTH1nth (E.coli endonuclease III)-like 1

Background

NTH1 (endonuclease III-like protein 1 or NTHL1) has both apurinic/apyrimidinic endonuclease activity and a DNA N-glycosylase activity. The C-O-P bond 3' to apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. NTH1 associates with 1 4Fe-4S cluster and this cluster has been proposed to involve in proper positioning of enzyme along the DNA strand. With potential for incising damaged DNA at cytosines, thymines and guanines, NTH1 acts on a damaged strand 5' from the damaged site, for playing essential role in repair of both oxidative DNA damage and spontaneous mutagenic lesions. Localized in the nucleus, NTH1 is widely expressed with highest levels in heart and its expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P

Publications for NTH1 Antibody (NB100-108)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-P
(2)
WB
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rat
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-108 Applications Species
Limpose KL, Trego KS, Li Z et al. Overexpression of the base excision repair NTHL1 glycosylase causes genomic instability and early cellular hallmarks of cancer. Nucleic Acids Res. Mar 7 2018 [PMID: 29522130] (IHC-P, Human) IHC-P Human
Ma H, Wang J, Abdel-Rahman SZ Et cl. Induction of base excision repair enzymes NTH1 and APE1 in rat spleen following aniline exposure. Toxicol Appl Pharmacol 2013 Jan 23 [PMID: 23352893] (IHC-P, Rat) IHC-P Rat
Tarry-Adkins JL, Martin-Gronert MS, Fernandez-Twinn DS et al. Poor maternal nutrition followed by accelerated postnatal growth leads to alterations in DNA damage and repair, oxidative and nitrosative stress, and oxidative defense capacity in rat heart. FASEB J. 2013 Jan [PMID: 23024373] (WB, Rat) WB Rat
Chang C-L, Ho M-C, Lee P-H et al. S1P5 is required for sphingosine 1-phosphate-induced autophagy in human prostate cancer PC-3 cells. Am J Physiol Cell Physiol 297(2):C451-458. 2009 [PMID: 19474291] (WB, Human) WB Human
Goto M, Shinmura K, Igarashi H et al. Altered expression of the human base excision repair gene NTH1 in gastric cancer. Carcinogenesis 30(8):1345-1352. 2009 [PMID: 19414504] (IHC, Human) IHC Human
O'reilly, M et al. p21(Cip1/WAF1/Sdi1) does not affect expression of base excision DNA repair enzymes during chronic oxidative stress. Antioxid Redox Signal. 7(5-6):719-25. 2005 May-Jun. [PMID: 15890018]
Lindberg C, Eriksson C, Van Dam AM et al. Neuronal expression of caspase-1 immunoreactivity in the rat central nervous system. J Neuroimmunol 2004 Jan [PMID: 14698852]
Wang AL, Lukas TJ et al. Age-related increase in mitochondrial DNA damage and loss of DNA repair capacity in the neural retina. Neurobiol Aging. 31(11):2002-10. 2010 Nov. [PMID: 1908429] (IHC, Rat, Mouse) IHC Rat, Mouse

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Product General Protocols

View specific protocols for NTH1 Antibody (NB100-108): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-108AF405
Alexa Fluor 488 NB100-108AF488
Alexa Fluor 647 NB100-108AF647
Alexa Fluor 700 NB100-108AF700
Biotin NB100-108B
DyLight 405 NB100-108V
DyLight 488 NB100-108G
DyLight 550 NB100-108R
DyLight 650 NB100-108C
FITC NB100-108F
HRP NB100-108H

Additional NTH1 Products

Array NB100-108

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Bioinformatics

Gene Symbol NTHL1