MTH1 Antibody

Images

 
Western Blot: MTH1 Antibody [NB100-109] - Analysis of MTH1 in 1. Ntera2, 2. MCF7 cell lysate, 3. A431 cell lysate and 4. COS7 cell lysate.
Immunocytochemistry/ Immunofluorescence: MTH1 Antibody [NB100-109] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Western Blot: MTH1 Antibody [NB100-109] - MTH1 antibody was tested in the labeled lanes.
Immunocytochemistry/ Immunofluorescence: MTH1 Antibody [NB100-109] - Detection of MTH1 (Green) in Hela cells using NB100-109. Nuclei (Blue) are counterstained with Hoechst 33258.
Immunocytochemistry/ Immunofluorescence: MTH1 Antibody [NB100-109] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated ...read more
Simple Western: MTH1 Antibody [NB100-109] - Simple Western lane view shows a specific band for MTH1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, Single Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MTH1 Antibody Summary

Immunogen
A C-terminal peptide derived from human MTH1, conjugated to KLH. [UniProt# P36639]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUDT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin
  • Single Cell Western 100 ug/ml
Application Notes
This MTH1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. In WB, a band can be seen at approx. 18kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
MTH1 Lysate (NBL1-13852)
MTH1 Lysate (NBP2-65737)
Publications
Read Publications using
NB100-109 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MTH1 Antibody

  • 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase
  • EC 3.6.1.-
  • MTH1Nucleoside diphosphate-linked moiety X motif 1
  • mutT human homolog 1,8-oxo-dGTPase
  • nucleoside diphosphate-linked moiety X-type motif 17,8-dihydro-8-oxoguanine triphosphatase
  • nudix (nucleoside diphosphate linked moiety X)-type motif 1,8-oxo-7,8-dihydroguanosine triphosphatase
  • Nudix motif 1

Background

MTH1 (human MutT Homolog 1) is an oxidized purine nucleoside triphosphatase enzyme that belongs to the Nudix hydrolase family and it acts as major mammalian detoxifier of the oxidized DNA precursor, 8-oxo-dGTP thereby exerting antimutagenic effects and suppressing cell dysfunction/death induced by oxidative stress. MTH1 hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thereby preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. MTH1 also hydrolyzes the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. 2-OH-dADP, 8-OH-dGDP and 8-OH-dGTP inhibit the 2-OH-dATPase activity whereas 8-OH-dGTPase activity is inhibited by 8-OH-dGDP, 2-OH-dADP and 2-OH-dATP. Localized mainly in the cytoplasm, MTH1 has been found in nucleus and mitochondria also, and is widely expressed with highest expression in thymus, testis, embryo, proliferating blood lymphocytes etc. Oxidative DNA damage, e.g. 8-oxoG is known to accumulate both in nuclear and mitochondrial genomes during aging, carcinogenesis or various neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease or amyotrophic lateral sclerosis and MTH1 deficiency can increase susceptibility to these oxidative damage mediated dysfunctions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MTH1 Antibody (NB100-109)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IB
(1)
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(10)
All Applications
Filter By Species
Human
(10)
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-109 Applications Species
Fan J, Zhao RQ, Parro C et al. Small molecule inducers of ABCA1 and apoE that act through indirect activation of the LXR pathway. J. Lipid Res. Mar 21 2018 [PMID: 29563219] (WB) WB
Hashiguchi K, Hayashi M, Sekiguchi M, Umezu K. The roles of human MTH1, MTH2 and MTH3 proteins in maintaining genome stability under oxidative stress. Mutat. Res. 2018 Jan 23 [PMID: 29482072] (WB, Human) WB Human
Page BDG, Valerie NCK, Wright RHG et al. Targeted NUDT5 inhibitors block hormone signaling in breast cancer cells Nat Commun 2018 Jan 17 [PMID: 29343827] (WB, Human) WB Human
Eshtad S, Mavajian Z, Rudd SG et al. hMYH and hMTH1 cooperate for survival in mismatch repair defective T-cell acute lymphoblastic leukemia. Oncogenesis. Dec 5 2016 [PMID: 27918552] (WB, Human) WB Human
Zhou H, Jian Y, Leng Y et al. Human MutT homologue 1 mRNA overexpression correlates to poor response of multiple myeloma. Int J Hematol 2017 Mar [PMID: 27848179] (Human) Human
Huber KV, Olek KM, Muller AC et al. Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling. Nat Methods 2015 Nov [PMID: 26389571]
Carter M, Jemth AS, Hagenkort A et al. Crystal structure, biochemical and cellular activities demonstrate separate functions of MTH1 and MTH2. Nat Commun. 2015 Aug 04 [PMID: 26238318] (WB, Human)

Details:
MTH1 antibody used for WB on U2OS cells that were transfected with MTH1 siRNA for 96 hours (Figure 4a). The primary antibody was used at a dilution of 1:1,000 with overnight incubation at 4C.
WB Human
Giribaldi MG, Munoz A, Halvorsen K et al. MTH1 expression is required for effective transformation by oncogenic HRAS. Oncotarget 2015 May 10 [PMID: 25893378] (Human) Human
Bonora M, Wieckowsk MR, Chinopoulos C et al. Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition. Oncogene 2015 Mar 19 [PMID: 25790189]
Patel A, Burton DG, Halvorsen K et al. MutT Homolog 1 (MTH1) maintains multiple KRAS-driven pro-malignant pathways. Oncogene 2014 Jul 14 [PMID: 25023700] (WB, Human)

Details:
MTH1 antibody used for WB and IHC-P in human species. In WB, the antibody used on lysates of A549, H358, H23 cells treated or not with lentiviral MTH1 shRNA, and several other experiments involving these cell lines. In IHC-P, the antibody used at 1:150 dilution on xenografts created from the transfected cell lines.
WB Human
Show All 18 Publications.

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Product General Protocols

View specific protocols for MTH1 Antibody (NB100-109): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-109AF405
Alexa Fluor 488 Labeled NB100-109AF488
Alexa Fluor 647 Labeled NB100-109AF647
Alexa Fluor 700 Labeled NB100-109AF700
Biotin Labeled NB100-109B
DyLight 405 Labeled NB100-109V
DyLight 488 Labeled NB100-109G
DyLight 550 Labeled NB100-109R
DyLight 650 Labeled NB100-109C
FITC Labeled NB100-109F
HRP Labeled NB100-109H

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Blogs on MTH1.

MTH1: Effects on DNA Damage Repair, Cancer and Neurodegeneration
MTH1 (human MutT Homolog 1) is a purine nucleoside triphosphatase enzyme and belongs to the Nudix hydrolase family. In mammalian systems, MTH is a major detoxifier of the oxidized DNA precursors, 8-oxo-dGTP, 8-oxo-dATP, and 2-OH-dATP and prevents the...  Read full blog post.

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Bioinformatics

Gene Symbol NUDT1
Entrez
Uniprot