MTH1 Antibody

Images

 
Western Blot: MTH1 Antibody [NB100-109] - Analysis of MTH1 in 1. Ntera2, 2. MCF7 cell lysate, 3. A431 cell lysate and 4. COS7 cell lysate.
Immunocytochemistry/ Immunofluorescence: MTH1 Antibody [NB100-109] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated ...read more
Western Blot: MTH1 Antibody [NB100-109] - MTH1 antibody was tested in the labeled lanes.
Immunocytochemistry/ Immunofluorescence: MTH1 Antibody [NB100-109] - Detection of MTH1 (Green) in Hela cells using NB100-109. Nuclei (Blue) are counterstained with Hoechst 33258.
Simple Western: MTH1 Antibody [NB100-109] - Simple Western lane view shows a specific band for MTH1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
1.0 mg/ml

MTH1 Antibody Summary

Immunogen
A C-terminal peptide derived from human MTH1, conjugated to KLH. [UniProt# P36639]
Clonality
Polyclonal
Host
Rabbit
Gene
NUDT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin
Application Notes
This MTH1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. In WB, a band can be seen at approx. 18kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-109 in the following applications:

Alternate Names for MTH1 Antibody

  • 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase
  • EC 3.6.1.-
  • MTH1Nucleoside diphosphate-linked moiety X motif 1
  • mutT human homolog 1,8-oxo-dGTPase
  • nucleoside diphosphate-linked moiety X-type motif 17,8-dihydro-8-oxoguanine triphosphatase
  • nudix (nucleoside diphosphate linked moiety X)-type motif 1,8-oxo-7,8-dihydroguanosine triphosphatase
  • Nudix motif 1

Background

MTH1 (human MutT Homolog 1) is an oxidized purine nucleoside triphosphatase enzyme that belongs to the Nudix hydrolase family and it acts as major mammalian detoxifier of the oxidized DNA precursor, 8-oxo-dGTP thereby exerting antimutagenic effects and suppressing cell dysfunction/death induced by oxidative stress. MTH1 hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thereby preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. MTH1 also hydrolyzes the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. 2-OH-dADP, 8-OH-dGDP and 8-OH-dGTP inhibit the 2-OH-dATPase activity whereas 8-OH-dGTPase activity is inhibited by 8-OH-dGDP, 2-OH-dADP and 2-OH-dATP. Localized mainly in the cytoplasm, MTH1 has been found in nucleus and mitochondria also, and is widely expressed with highest expression in thymus, testis, embryo, proliferating blood lymphocytes etc. Oxidative DNA damage, e.g. 8-oxoG is known to accumulate both in nuclear and mitochondrial genomes during aging, carcinogenesis or various neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease or amyotrophic lateral sclerosis and MTH1 deficiency can increase susceptibility to these oxidative damage mediated dysfunctions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MTH1 Antibody (NB100-109)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IB
(1)
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-109 Applications Species
Huber KV, Olek KM, Muller AC et al. Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling. Nat Methods 2015 Nov [PMID:26389571]
Carter M, Jemth AS, Hagenkort A et al. Crystal structure, biochemical and cellular activities demonstrate separate functions of MTH1 and MTH2. Nat Commun. 2015 Aug 04 [PMID:26238318] (WB, Human) WB Human
Giribaldi MG, Munoz A, Halvorsen K et al. MTH1 expression is required for effective transformation by oncogenic HRAS. Oncotarget 2015 May 10 [PMID:25893378] (Human) Human
Patel A, Burton DG, Halvorsen K et al. MutT Homolog 1 (MTH1) maintains multiple KRAS-driven pro-malignant pathways. Oncogene 2014 Jul 14 [PMID:25023700] (WB, Human) WB Human
Huber KV, Salah E, Radic B et al. Stereospecific targeting of MTH1 by (S)-crizotinib as an anticancer strategy. Nature 2014 Apr 10 [PMID:24695225] (WB, Human) WB Human
Sheng Z, Oka S, Tsuchimoto D et al. 8-Oxoguanine causes neurodegeneration during MUTYH-mediated DNA base excision repair J Clin Invest 2012 Dec 3 [PMID:23143307] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Chang IY, Kim JH, Cho KW, Yoon SP. Acute responses of DNA repair proteins and StarD6 in rat hippocampus after domoic acid-induced excitotoxicity Acta Histochem 2012 Aug 7 [PMID:22883302] (WB, Mouse) WB Mouse
Bruel L, Sulzenbacher G, Cervera Tison M et al. alpha-Galactosidase/sucrose kinase (AgaSK), a novel bifunctional enzyme from the human microbiome coupling galactosidase and kinase activities. J Biol Chem 2011 Nov 25 [PMID:21931163]
Rai P, Young JJ, Burton DG et al. Enhanced elimination of oxidized guanine nucleotides inhibits oncogenic RAS-induced DNA damage and premature senescence. Oncogene. 2010 Nov 15. [PMID:21076467] (IB, Human) IB Human
Rai P et al. Continuous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence. Proc Natl Acad Sci U S A;106(1):169-74. 2009 Jan 6. [PMID:19118192]
Show All 12 Publications.

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Product General Protocols

View specific protocols for MTH1 Antibody (NB100-109): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MTH1 Antibody (NB100-109) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-109AF405
Alexa Fluor 488 Labeled NB100-109AF488
Alexa Fluor 647 Labeled NB100-109AF647
Alexa Fluor 700 Labeled NB100-109AF700
Biotin Labeled NB100-109B
DyLight 405 Labeled NB100-109V
DyLight 488 Labeled NB100-109G
DyLight 550 Labeled NB100-109R
DyLight 650 Labeled NB100-109C
FITC Labeled NB100-109F
HRP Labeled NB100-109H

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Blogs on MTH1.

MTH1: Effects on DNA Damage Repair, Cancer and Neurodegeneration
MTH1 (human MutT Homolog 1) is a purine nucleoside triphosphatase enzyme and belongs to the Nudix hydrolase family. In mammalian systems, MTH is a major detoxifier of the oxidized DNA precursors, 8-oxo-dGTP, 8-oxo-dATP, and 2-OH-dATP and prevents the...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol NUDT1
Entrez
Uniprot

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