MTH1 Antibody - BSA Free

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Genetic Strategies: Knockout Validated: MTH1 Antibody [NB100-109] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MTH1 knockout (KO) HEK293T cell line. PVDF membrane was ...read more
Simple Western: MTH1 Antibody [NB100-109] - Simple Western lane view shows a specific band for MTH1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Western Blot: MTH1 Antibody [NB100-109] - The cells from esophageal cancer cell lines: KYSE-450, EC109 and EC9706 (d, e), liver cancer cell lines: SMMC-7721, HepG2 and ZIP177 (f, g), gastric cancer cell lines: MGC-803, ...read more
Biological Strategies: Western Blot: MTH1 Antibody [NB100-109] - The antioxidant pathways are activated during EBV infection and are required for growth transformation. Freshly isolated B-lymphocytes infected ...read more
Immunocytochemistry/ Immunofluorescence: MTH1 Antibody [NB100-109] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Western Blot: MTH1 Antibody [NB100-109] - EBNA1 promotes the upregulation of oxidative DNA damage repair pathways in EBV converted cell lines and EBV positive BLs. Densitometry quantification of the specific bands. The ...read more
Western Blot: MTH1 Antibody [NB100-109] - MTH1 antibody was tested in the labeled lanes.
Western Blot: MTH1 Antibody [NB100-109] - Analysis of MTH1 in 1. Ntera2, 2. MCF7 cell lysate, 3. A431 cell lysate and 4. COS7 cell lysate.
Immunocytochemistry/ Immunofluorescence: MTH1 Antibody [NB100-109] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated ...read more
Knockdown Validated: MTH1 Antibody [NB100-109] - MTH1 suppression reduces the two gastric cancer cell survival. MGC-803, HGC-27 and GES-1 cells were treated by MTH1 specific siRNA 1 and #2 for 72h. Non-targeting ...read more
Knockdown Validated: MTH1 Antibody [NB100-109] - Simultaneous suppression of MTH1 and MYH was efficiently achieved using a two-vector system. Steps of cell line establishment are illustrated here. Top 15% of cells ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IB, ICC/IF, IHC, IHC-Fr, IHC-P, KD, KO, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

MTH1 Antibody - BSA Free Summary

Immunogen
A C-terminal peptide derived from human MTH1, conjugated to KLH. [UniProt# P36639]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUDT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Knockout Validated
  • Simple Western 1:50
  • Single Cell Western 100 ug/ml
  • Western Blot 1:500
Application Notes
This MTH1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. In WB, a band can be seen at approx. 18kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in immunoblotting reported in scientific literature (PMID: 21076467).
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Publications
Read Publications using
NB100-109 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 12706856).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MTH1 Antibody - BSA Free

  • 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase
  • EC 3.6.1.-
  • MTH1Nucleoside diphosphate-linked moiety X motif 1
  • mutT human homolog 1,8-oxo-dGTPase
  • nucleoside diphosphate-linked moiety X-type motif 17,8-dihydro-8-oxoguanine triphosphatase
  • nudix (nucleoside diphosphate linked moiety X)-type motif 1,8-oxo-7,8-dihydroguanosine triphosphatase
  • Nudix motif 1

Background

MTH1 (human MutT Homolog 1) is an oxidized purine nucleoside triphosphatase enzyme that belongs to the Nudix hydrolase family and it acts as major mammalian detoxifier of the oxidized DNA precursor, 8-oxo-dGTP thereby exerting antimutagenic effects and suppressing cell dysfunction/death induced by oxidative stress. MTH1 hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thereby preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. MTH1 also hydrolyzes the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. 2-OH-dADP, 8-OH-dGDP and 8-OH-dGTP inhibit the 2-OH-dATPase activity whereas 8-OH-dGTPase activity is inhibited by 8-OH-dGDP, 2-OH-dADP and 2-OH-dATP. Localized mainly in the cytoplasm, MTH1 has been found in nucleus and mitochondria also, and is widely expressed with highest expression in thymus, testis, embryo, proliferating blood lymphocytes etc. Oxidative DNA damage, e.g. 8-oxoG is known to accumulate both in nuclear and mitochondrial genomes during aging, carcinogenesis or various neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease or amyotrophic lateral sclerosis and MTH1 deficiency can increase susceptibility to these oxidative damage mediated dysfunctions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-109
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IB, ICC/IF, IHC, IHC-Fr, IHC-P, KD, KO, Single-Cell Western

Publications for MTH1 Antibody (NB100-109)(31)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


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IB
(1)
ICC/IF
(1)
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(1)
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(1)
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(1)
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(20)
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Human
(18)
Mouse
(3)
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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using NB100-109 Applications Species
Bialkowski K, Szpila A Specific 8-oxo-dGTPase activity of MTH1 (NUDT1) protein as a quantitative marker and prognostic factor in human colorectal cancer Free radical biology & medicine Oct 5 2021 [PMID: 34624481]
Karsten S, Fiskesund R, Zhang XM Et al. MTH1 as a target to alleviate T cell driven diseases by selective suppression of activated T cells Cell death and differentiation Aug 27 2021 [PMID: 34453118] (WB, Human) WB Human
Zhang L, Misiara L, Samaranayake GJ, et al. OGG1 co-inhibition antagonizes the tumor-inhibitory effects of targeting MTH1 Redox biology Jan 2 2021 [PMID: 33450725] (WB, Human) WB Human
Arczewska KD, Krasuska W, Stachurska A et al. hMTH1 and GPX1 expression in human thyroid tissue is interrelated to prevent oxidative DNA damage DNA Repair (Amst.) Aug 20 2020 [PMID: 32877752] (WB, Human) WB Human
Godoy PRDV, Pour Khavari A, Rizzo M et al. Targeting NRF2, Regulator of Antioxidant System, to Sensitize Glioblastoma Neurosphere Cells to Radiation-Induced Oxidative Stress Oxid Med Cell Longev Jun 15 2020 [PMID: 32617133] (WB, Human) WB Human
Rudd SG, Gad H, Sanjiv K et al. MTH1 inhibitor TH588 disturbs mitotic progression and induces mitosis-dependent accumulation of genomic 8-oxodG Cancer Res. Apr 20 2020 [PMID: 32312836]
Moukengue B, Brown HK, Charrier C et al. TH1579, MTH1 inhibitor, delays tumour growth and inhibits metastases development in osteosarcoma model EBioMedicine Mar 6 2020 [PMID: 32151797] (Mouse) Mouse
Bialkowski K, Kasprzak KS A profile of 8-oxo-dGTPase activities in the NCI-60 human cancer panel: Meta-analytic insight into the regulation and role of MTH1 (NUDT1) gene expression in carcinogenesis Free Radic. Biol. Med. Dec 25 2019 [PMID: 31883466] (WB, Human) WB Human
Wang J, Nagy N, Masucci MG The Epstein-Barr virus nuclear antigen-1 upregulates the cellular antioxidant defense to enable B-cell growth transformation and immortalization Oncogene Sep 11 2019 [PMID: 31511648] (WB) WB
McPherson LA, Troccoli CI, Ji D et al. Increased MTH1-specific 8-oxodGTPase activity is a hallmark of cancer in colon, lung and pancreatic tissue DNA Repair (Amst.) Jul 5 2019 [PMID: 31311767] (WB, Human) WB Human
Show All 31 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on MTH1.

MTH1: Effects on DNA Damage Repair, Cancer and Neurodegeneration
MTH1 (human MutT Homolog 1) is a purine nucleoside triphosphatase enzyme and belongs to the Nudix hydrolase family. In mammalian systems, MTH is a major detoxifier of the oxidized DNA precursors, 8-oxo-dGTP, 8-oxo-dATP, and 2-OH-dATP and prevents the ...  Read full blog post.

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Bioinformatics

Gene Symbol NUDT1
Entrez
Uniprot