NT-4 Antibody (36507)

• Reactivity: Hu
• Applications: WB
• Clone: 36507
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NT-4 Antibody (36507) Summary

• Immunogen: E. coli-derived recombinant human NT-4
  Gly81-Ala210 (predicted)
  Accession # P34130
• Specificity: Detects human NT-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) BDNF or rhNT-3 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NTF4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NT-4 Antibody (36507)

• GLC10
• GLC1O
• neurotrophic factor 4
• neurotrophic factor 5
• neurotrophin 4
• neurotrophin 5 (neurotrophin 4/5)
• neurotrophin-4
• Neurotrophin-5
• Neutrophic factor 4
• NT4
• NT-4
• NT-4/5
• NT-5
• NTF4
• NTF5
• NTF5NT5

Background

Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds (1‑3). The human NT-4 cDNA encodes a 210 amino acid (aa) precursor that includes a 24 aa signal sequence, a 56 aa propeptide, and a 130 aa mature protein (4, 5). NT-4 is synthesized as a 28 kDa prepropeptide that is proteolytically processed to generate the mature protein. Mature human NT-4 shares 48‑52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3 (6). NT-4 binds and induces receptor dimerization and activation of TrkB (4, 7). NT-4 promotes the development and survival of selected peripheral and CNS neurons (8‑10). BDNF, which also activates TrkB, overlaps with many but not all NT-4 functions, a distinction that is likely due to differences in expression patterns (8‑10). NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death (11). It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle (12, 13). NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration (14‑16).

1. Lessmann, V. et al. (2003) Prog. Neurobiol. 69:341.
2. Tabakman, R. et al. (2004) Prog. Brain Res. 146:387.
3. Botchkarev, V.A. et al. (2004) Prog. Brain Res. 146:493.
4. Ip, N.Y. et al. (1992) Proc. Natl. Acad. Sci. 89:3060.
5. Berkemeier, L.R. et al. (1991) Neuron 7:857.
6. Radziejewski, C. and R.C. Robinson (1993) Biochemistry 32:13350.
7. Vesa, J. et al. (2000) J. Biol. Chem. 275:24414.
8. Davies, A.M. et al. (1993) J. Neurosci. 13:4961.
9. Stucky, C.L. et al. (1998) J. Neurosci. 18:7040.
10. Fan, G. et al. (2000) Nat. Neurosci. 3:350.
11. Choi, S.Y. et al. (2004) J. Neurochem. 88:708.
12. Botchkarev, V.A. et al. (1999) Lab. Invest. 79:557.
13. Botchkarev, V.A. et al. (1999) FASEB J. 13:395.
14. Laurenzi, M.A. et al. (1998) J. Leukoc. Biol. 64:228.
15. Moalem, G. et al. (2000) J. Autoimmun. 15:331.
16. Nassenstein, C. et al. (2003) J. Exp. Med. 198:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NT-4 Antibody (MAB2681)(1)

Publication using MAB2681

• Rose JB, Crews L, Rockenstein E, Adame A, Mante M, Hersh LB, Gage FH, Spencer B, Potkar R, Marr RA, Masliah E Neuropeptide Y fragments derived from neprilysin processing are neuroprotective in a transgenic model of Alzheimer's disease. J. Neurosci., 2009-01-28;29(4):1115-25. 2009-01-28 [PMID: 19176820] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: NTF4
• Uniprot:
  • P34130()