Nox4 Antibody - BSA Free

Western Blot: Nox4 Antibody [NBP2-41293] - Analysis of NOX4 in Jurkat cell lysate with NOX4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry: Nox4 Antibody - BSA Free [NBP2-41293] - Immunohistochemistry of Nox4 in human spleen tissue with Nox4 antibody at 5 u/ml.

Immunocytochemistry/ Immunofluorescence: Nox4 Antibody - BSA Free [NBP2-41293] - Immunofluorescence of Nox4 in human spleen tissue with Nox4 antibody at 20 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Nox4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Nox4 Antibody - BSA Free (NBP2-41293) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Nox4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid peptide near the amino terminus of human NOX4. The immunogen is located within amino acids 70 - 120 of NOX4. Amino Acid Squence: RGSQKVPSRRTRRL
• Specificity: At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOX4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Nox4 Antibody - BSA Free

• EC 1.6.3
• Kidney oxidase-1
• Kidney superoxide-producing NADPH oxidase
• KOX
• KOX-1
• KOX-1Renal NAD(P)H-oxidase
• NADPH oxidase 4
• Nox4
• RENOX
• RENOXEC 1.6.3.-

Background

NOX4 (RENOX) is a renal gp91-phox homolog which functions in the kidney as an oxygen sensor and regulates the synthesis of erythropoietin. This protein is highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex.

NOX-4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of NOX4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases NOX4 expression, which indicates that NOX4 has a role in vascular oxidative stress response. NOX4 has a role as a redox messenger in the activation of intracellular signaling pathways leading (or contributing) to mitochondriogenesis, cell survival, and differentiation in hematopoietic stem cells. Data suggest that NOX4 provides a novel link between the insulin receptor and the generation of cellular reactive oxygen species that enhances insulin signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nox4 Antibody (NBP2-41293)(1)

Publication using NBP2-41293

• Murley JS, Arbiser JL, Weichselbaum RR, Grdina DJ. ROS Modifiers and NOX4 Affect the Expression of the Survivin-Associated Radio-Adaptive Response. Free Radic. Biol. Med. 2018-04-13 [PMID: 29660403] (Human)

Bioinformatics

• Gene Symbol: NOX4