Notch-1 Antibody - BSA Free

Western Blot: Notch-1 Antibody [NBP2-24771] - Analysis of Notch1 in HEK293 cells transfected with full length Notch 1 A) untreated and B) treated with 5 mM EDTA for 15 minutes, using this antibody at 3 ug/mL. The EDTA treatment increases the detected level of Notch1-cv.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Notch-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Notch-1 Antibody - BSA Free (NBP2-24771) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Within the range of amino acids 1755-1780 of human Notch1 were used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOTCH1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 3 - 5 ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.2% Gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Notch-1 Antibody - BSA Free

• EC 2.1.2.11
• EC 3.4.21.68
• hN1
• Notch (Drosophila) homolog 1 (translocation-associated)
• notch 1Notch homolog 1, translocation-associated (Drosophila)
• Notch homolog 1, translocation-associated
• Notch1
• Notch-1
• TAN1
• TAN1neurogenic locus notch homolog protein 1
• Translocation-associated notch protein TAN-1

Background

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Notch-1 Antibody (NBP2-24771). (Showing 1 - of FAQ).

Bioinformatics

• Gene Symbol: NOTCH1
• Uniprot:
  • P46531()