Notch-1 Antibody (OTI3E12) - Azide and BSA Free

Western Blot: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1.

Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of HepG2 cells using anti Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded pancreas using anti-Notch1 mouse monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded kidney using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded lung using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded breast using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Carcinoma of lung using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded colon using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded endometrium using anti-Notch1 mouse monoclonal antibody.

Immunohistochemistry: Notch-1 Antibody (OTI3E12) - Azide and BSA Free [NBP2-73042] - Staining of paraffin-embedded thyroid using anti-Notch1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, MA
• Clone: OTI3E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Notch-1 Antibody (OTI3E12) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Notch-1 Antibody (OTI3E12) - Azide and BSA Free (NBP1-48289) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOTCH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50RPPA 1:75
  • Immunohistochemistry-Paraffin
  • Reverse Phase Protein Array 1:75
  • Western Blot 1:1000
• Theoretical MW: 270.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Notch-1 Antibody (OTI3E12) - Azide and BSA Free

• EC 2.1.2.11
• EC 3.4.21.68
• hN1
• Notch (Drosophila) homolog 1 (translocation-associated)
• notch 1Notch homolog 1, translocation-associated (Drosophila)
• Notch homolog 1, translocation-associated
• Notch1
• Notch-1
• TAN1
• TAN1neurogenic locus notch homolog protein 1
• Translocation-associated notch protein TAN-1

Background

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Notch-1 Antibody (NBP2-73042). (Showing 1 - of FAQ).

Bioinformatics

• Gene Symbol: NOTCH1