Notch-1 Antibody (OTI3E12)

Images

 
Western Blot: Notch-1 Antibody (3E12) [NBP1-48289] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of HepG2 cells using anti Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded thyroid using anti-Notch1 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded breast using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of lung using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded colon using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded endometrium using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded kidney using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded lung using anti-Notch1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Notch-1 Antibody (3E12) [NBP1-48289] - Staining of paraffin-embedded pancreas using anti-Notch1 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI3E12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Notch-1 Antibody (OTI3E12) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1.
Specificity
This antibody is specific for Homo sapiens Notch homolog 1, translocation-associated (Drosophila) (NOTCH1).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NOTCH1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
272 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Artery Lysate (NB820-60569)
Notch-1 Lysate (NBP2-64906)
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-48289 in the following applications:

Publications
Read Publications using
NBP1-48289 in the following applications:

  • 2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Notch-1 Antibody (OTI3E12)

  • EC 2.1.2.11
  • EC 3.4.21.68
  • hN1
  • Notch (Drosophila) homolog 1 (translocation-associated)
  • notch 1Notch homolog 1, translocation-associated (Drosophila)
  • Notch homolog 1, translocation-associated
  • Notch1
  • Notch-1
  • TAN1
  • TAN1neurogenic locus notch homolog protein 1
  • Translocation-associated notch protein TAN-1

Background

Notch1 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-47791
Species: Hu, Mk
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
NB100-93551
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF1190
Species: Rt
Applications: WB, Flow, IHC, Block, CyTOF-ready
AF4079
Species: Hu
Applications: WB, ICC
H00008209-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NB300-749
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
H00004854-M01
Species: Hu
Applications: WB, ELISA
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-59631
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, ICC
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-13075
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
AF1389
Species: Mu
Applications: WB, ICC
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-86337
Species: Hu, Mu, Ha
Applications: IHC, IHC-Fr, IHC-P
NBP2-41305
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF

Publications for Notch-1 Antibody (NBP1-48289)(3)

Reviews for Notch-1 Antibody (NBP1-48289) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 review tested in 1 species: Human.

Reviews using NBP1-48289:
Filter by Applications
WB
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Alissa Taylor
WB Human 10/28/2014
View

Summary

ApplicationWestern Blot
Sample Testedfull length human Notch1 plasmid transfected Hela whole cell lysate
SpeciesHuman

Blocking

Blocking Details5% non fat milk 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:1000 in blocking buffer, 1 hour at room temperature
 
reviewed by:
Alissa Taylor
IHC-P 10/28/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Notch-1 Antibody (NBP1-48289) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Notch-1 Antibody (NBP1-48289)

Discover related pathways, diseases and genes to Notch-1 Antibody (NBP1-48289). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Notch-1 Antibody (NBP1-48289)

Discover more about diseases related to Notch-1 Antibody (NBP1-48289).
 

Pathways for Notch-1 Antibody (NBP1-48289)

View related products by pathway.

PTMs for Notch-1 Antibody (NBP1-48289)

Learn more about PTMs related to Notch-1 Antibody (NBP1-48289).
 

Research Areas for Notch-1 Antibody (NBP1-48289)

Find related products by research area.

Blogs on Notch-1.

Notch1 - A multifunctional transmembrane receptor
Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

 
Alissa Taylor
10/28/2014
Application: WB
Species: Human

 
Alissa Taylor
10/28/2014
Application: IHC-P
Species:

Bioinformatics

Gene Symbol NOTCH1
Entrez
Uniprot