Noggin Antibody (OTI1H8)

Western Blot: Noggin Antibody (OTI1H8) [NBP1-47881] - Noggin Antibody (1H8) [NBP1-47881] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Noggin monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Noggin Antibody (OTI1H8) [NBP1-47881] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Noggin.

Immunohistochemistry-Paraffin: Noggin Antibody (1H8) [NBP1-47881] - Staining of paraffin-embedded Human Ovary tissue using anti-Noggin mouse monoclonal antibody.

Western Blot: Noggin Antibody (1H8) [NBP1-47881] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Noggin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Noggin.

Western Blot: Noggin Antibody (1H8) [NBP1-47881] - Noggingin antibody (1H8) at 1:40000 + Recombinant human Noggingin.

Immunohistochemistry-Paraffin: Noggin Antibody (1H8) [NBP1-47881] - Staining of paraffin-embedded Human lung tissue using anti-Noggin mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clone: OTI1H8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Noggin Antibody (OTI1H8) Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 28-232 of human noggin (NP_005441) produced in E.coli.
• Specificity: This antibody is specific for Homo sapiens noggin (NOG).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:20000-40000
• Theoretical MW: 23.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Noggin Antibody (OTI1H8)

• NOG
• Noggin
• SYM1
• symphalangism 1 (proximal)
• synostoses (multiple) syndrome 1
• SYNS1
• SYNS1A

Background

Noggin is a secreted polypeptide which binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, it may have a principal role in creating morphogenic gradients. Noggin appears to have pleiotropic effect, both early in development as well as in later stages. The results of the mouse knockout of the ortholog suggest that noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOG
• Entrez:
  • 9241(Human)