NOD2 Antibody

Images

 
Western Blot: NOD2 Antibody [NBP2-27328] - Analysis of NOD2 using this antibody at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M ...read more
Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-27328] - Formalin-fixed, paraffin-embedded normal human colon stained for NOD2 expression using this antibody at 1:2000. A, tissue core from a colon tissue microarray. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NOD2 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 582-610 of human NOD2/CARD15 was used as immunogen; GenBank no. NP_071445 NOD2 is a 1040 amino acid protein. The immunogen sequence is 100% conserved in certain NOD2 isoforms and mutations. The immunogen sequence corresponds to amino acids 45-63 of a short form of NOD2. This NOD2 short form is 273 amino acids; GenBank no.AAk70868.1 aa 582-600; N-Terminus; human
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOD2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000~
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000~
  • Immunoprecipitation 1:50-1:200
Application Notes
NOD2 produces, by alternative splicing, 13 different transcripts, all with introns, putatively encoding 13 different protein isoforms. Therefore, the molecular weights observed for Nod2 on Western blot may vary.
Publications
Read Publications using
NBP2-27328 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 20615568).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50 ul neat whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for NOD2 Antibody

  • BLAU
  • CARD15caspase recruitment domain protein 15
  • caspase recruitment domain family, member 15
  • Caspase recruitment domain-containing protein 15
  • CDACUG
  • CLR16.3
  • IBD1NLR family, CARD domain containing 2
  • Inflammatory bowel disease protein 1
  • NLRC2
  • NOD-like receptor C2
  • nucleotide-binding oligomerization domain 2
  • nucleotide-binding oligomerization domain containing 2
  • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
  • nucleotide-binding oligomerization domain-containing protein 2
  • PSORAS1NOD2B

Background

Innate immunity is present in all animals and is the common mode of defense against microorganisms. It detects microorganisms by specific proteins called pattern-recognition molecules (PRMs) (reviewed in Werts et al. 2006). There is a limited set of PRMs in each animal genome, and it has been postulated that the PRMs were evolutionarily selected to detect conserved components or motifs of microorganisms called pathogen-associated molecular patterns (PAMPs). PAMPs are found in a wide range of microorganisms and recognition of PAMPs by PRMs activates inflammatory signaling pathways, thereby stimulating an immune response. NOD (nucleotide-binding oligomerization domain) proteins are a family of cytosolic proteins which have been implicated in innate recognition of bacteria, the induction of inflammatory responses, and the regulation of caspase activation and apoptosis. NOD2/CARD15 is a PRM that recognizes specific peptidoglycan (PGN) components of bacterial cell walls (reviewed in Strober et al. 2006, and Inohara et al. 2003). NOD2 is expressed in cells that are exposed to PGN under physiological conditions including antigen-presenting cells (APCs) such as macrophages and dendritic cells, and epithelial cells (reviewed in Strober et al. 2006). NOD2 is a for thought to play a role in the pathogenesis of human gastrointestinal disease. Polymorphisms and mutations in NOD2 are associated with susceptibility to Crohn's disease, a chronic inflammatory bowel disease (Leung et al., 2006). Additionally, polymorphisms in NOD2 have also been found to be associated with Graft-versus-Host Disease (GVHD) (Holler et al., 2004)This association of NOD2 gene polymorphisms with gastrointestinal disease and GVHD suggests that NOD2 gene polymorphisms play a role in pro-inflammatory diseases. NBP2-27328 recognizes NOD2, a 1040 amino acid protein. NBP2-27328 also recognizes NOD2 alternatively spliced and mutated forms which contain the peptide immunogen sequence including NOD2 short, a 273 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00003347-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
NBP2-25255
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P
NBP2-25208
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
7270-IL/CF
Species: Hu
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-85466
Species: Hu, Mu, Fe
Applications: WB, IHC, IHC-P
6507-IL/CF
Species: Hu
NBP2-67605
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
MAB342
Species: Hu
Applications: WB, AgAct
NB100-77417
Species: Hu, Mu, Rt
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, In vivo, CyTOF-ready
NBP1-88220
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
NB100-1598
Species: Hu, Mu
Applications: WB, Flow, ICC/IF
NBP2-98675
Species: Hu
Applications: WB, IHC, IHC-P, IP
Supplier Logo

Publications for NOD2 Antibody (NBP2-27328)(3)

Reviews for NOD2 Antibody (NBP2-27328) (0)

There are no reviews for NOD2 Antibody (NBP2-27328). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NOD2 Antibody (NBP2-27328). (Showing 1 - 1 of 1 FAQs).

  1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
    • For detecting endogenous NOD2 in human samples, I would recommend NB100-524 (http://www.novusbio.com/NB100-524). This antibody has been validated for use in WB, IHC-Paraffin and IP.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional NOD2 Products

Bioinformatics Tool for NOD2 Antibody (NBP2-27328)

Discover related pathways, diseases and genes to NOD2 Antibody (NBP2-27328). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NOD2 Antibody (NBP2-27328)

Discover more about diseases related to NOD2 Antibody (NBP2-27328).
 

Pathways for NOD2 Antibody (NBP2-27328)

View related products by pathway.

PTMs for NOD2 Antibody (NBP2-27328)

Learn more about PTMs related to NOD2 Antibody (NBP2-27328).
 

Research Areas for NOD2 Antibody (NBP2-27328)

Find related products by research area.

Blogs on NOD2.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

NOD2 - inflammatory signaling and NFkB activation
Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) is an intracellular pattern recognition receptor (PRR) that plays an important role in recognizing bacterial pathogens and initiating an immune response. As a PRR, NOD2 recogniz...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NOD2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NOD2
Uniprot