NOD2 Antibody

Western Blot: NOD2 Antibody [NBP2-27328] - Analysis of NOD2 using this antibody at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.

Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-27328] - Formalin-fixed, paraffin-embedded normal human colon stained for NOD2 expression using this antibody at 1:2000. A, tissue core from a colon tissue microarray. A1, high magnification of A. Hematoxylin-eosin counterstain.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-Fr, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NOD2 Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acids 582-610 of human NOD2/CARD15 was used as immunogen; GenBank no. NP_071445 NOD2 is a 1040 amino acid protein. The immunogen sequence is 100% conserved in certain NOD2 isoforms and mutations. The immunogen sequence corresponds to amino acids 45-63 of a short form of NOD2. This NOD2 short form is 273 amino acids; GenBank no.AAk70868.1 aa 582-600; N-Terminus; human
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOD2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000~
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000~
• Application Notes: NOD2 produces, by alternative splicing, 13 different transcripts, all with introns, putatively encoding 13 different protein isoforms. Therefore, the molecular weights observed for Nod2 on Western blot may vary.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 20615568).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50 ul neat whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for NOD2 Antibody

• BLAU
• CARD15caspase recruitment domain protein 15
• caspase recruitment domain family, member 15
• Caspase recruitment domain-containing protein 15
• CDACUG
• CLR16.3
• IBD1NLR family, CARD domain containing 2
• Inflammatory bowel disease protein 1
• NLRC2
• NOD-like receptor C2
• nucleotide-binding oligomerization domain 2
• nucleotide-binding oligomerization domain containing 2
• nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
• nucleotide-binding oligomerization domain-containing protein 2
• PSORAS1NOD2B

Background

Innate immunity is present in all animals and is the common mode of defense against microorganisms. It detects microorganisms by specific proteins called pattern-recognition molecules (PRMs) (reviewed in Werts et al. 2006). There is a limited set of PRMs in each animal genome, and it has been postulated that the PRMs were evolutionarily selected to detect conserved components or motifs of microorganisms called pathogen-associated molecular patterns (PAMPs). PAMPs are found in a wide range of microorganisms and recognition of PAMPs by PRMs activates inflammatory signaling pathways, thereby stimulating an immune response. NOD (nucleotide-binding oligomerization domain) proteins are a family of cytosolic proteins which have been implicated in innate recognition of bacteria, the induction of inflammatory responses, and the regulation of caspase activation and apoptosis. NOD2/CARD15 is a PRM that recognizes specific peptidoglycan (PGN) components of bacterial cell walls (reviewed in Strober et al. 2006, and Inohara et al. 2003). NOD2 is expressed in cells that are exposed to PGN under physiological conditions including antigen-presenting cells (APCs) such as macrophages and dendritic cells, and epithelial cells (reviewed in Strober et al. 2006). NOD2 is a for thought to play a role in the pathogenesis of human gastrointestinal disease. Polymorphisms and mutations in NOD2 are associated with susceptibility to Crohn's disease, a chronic inflammatory bowel disease (Leung et al., 2006). Additionally, polymorphisms in NOD2 have also been found to be associated with Graft-versus-Host Disease (GVHD) (Holler et al., 2004)This association of NOD2 gene polymorphisms with gastrointestinal disease and GVHD suggests that NOD2 gene polymorphisms play a role in pro-inflammatory diseases. NBP2-27328 recognizes NOD2, a 1040 amino acid protein. NBP2-27328 also recognizes NOD2 alternatively spliced and mutated forms which contain the peptide immunogen sequence including NOD2 short, a 273 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NOD2 Antibody (NBP2-27328)(3)

Publications using NBP2-27328

• Scott MJ, Chen C, Sun Q, Billiar TR. Hepatocytes express functional NOD1 and NOD2 receptors: a role for NOD1 in hepatocyte CC and CXC chemokine production. J Hepatol. 2010-10-01 [PMID: 20615568] (WB, Mouse)
  
  Details:
  Products cited: NOD1/CARD4 (IMG-5224A) and NOD2/CARD15 (IMG-5738). WB: Mouse C57BL/6 hepatocyte NOD1 & NOD2 siRNA transfected cell line, Fig 4C. Note: The NOD1 (IMG-5224A) and NOD2 (IMG-5738) antibodies were siRNA validated by WB in mouse C57BL/6 hepatocy
• Scurrell E, Stanley R, Sch0niger S. Immunohistochemical detection of NOD1 and NOD2 in the healthy murine and canine eye. Vet Ophthalmol. 2009-07-01 [PMID: 19604345]
• Lin ZM, Song Z, Qin W et al. Expression of nucleotide-binding oligomerization domain 2 in normal human dental pulp cells and dental pulp tissues. J Endod. 2009-06-01 [PMID: 19482182] (WB, Human)
  
  Details:
  Product cited: NOD2/CARD15 (IMG-5738).1. WB: Human dental pulp cells (HDPC), Fig 3.

FAQs for NOD2 Antibody (NBP2-27328). (Showing 1 - 1 of 1 FAQs).

1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
   • For detecting endogenous NOD2 in human samples, I would recommend NB100-524 (http://www.novusbio.com/NB100-524). This antibody has been validated for use in WB, IHC-Paraffin and IP.

Bioinformatics

• Gene Symbol: NOD2
• Uniprot:
  • Q9HC29(Human)