Western Blot: NOD2 Antibody [NBP2-27328] - Analysis of NOD2 using this antibody at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M ...read more
Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-27328] - Formalin-fixed, paraffin-embedded normal human colon stained for NOD2 expression using this antibody at 1:2000. A, tissue core from a colon tissue microarray. ...read more
A synthetic peptide corresponding to amino acids 582-610 of human NOD2/CARD15 was used as immunogen; GenBank no. NP_071445 NOD2 is a 1040 amino acid protein. The immunogen sequence is 100% conserved in certain NOD2 isoforms and mutations. The immunogen sequence corresponds to amino acids 45-63 of a short form of NOD2. This NOD2 short form is 273 amino acids; GenBank no.AAk70868.1 aa 582-600; N-Terminus; human
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NOD2 produces, by alternative splicing, 13 different transcripts, all with introns, putatively encoding 13 different protein isoforms. Therefore, the molecular weights observed for Nod2 on Western blot may vary.
Read Publications using NBP2-27328 in the following applications:
nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
nucleotide-binding oligomerization domain-containing protein 2
Innate immunity is present in all animals and is the common mode of defense against microorganisms. It detects microorganisms by specific proteins called pattern-recognition molecules (PRMs) (reviewed in Werts et al. 2006). There is a limited set of PRMs in each animal genome, and it has been postulated that the PRMs were evolutionarily selected to detect conserved components or motifs of microorganisms called pathogen-associated molecular patterns (PAMPs). PAMPs are found in a wide range of microorganisms and recognition of PAMPs by PRMs activates inflammatory signaling pathways, thereby stimulating an immune response. NOD (nucleotide-binding oligomerization domain) proteins are a family of cytosolic proteins which have been implicated in innate recognition of bacteria, the induction of inflammatory responses, and the regulation of caspase activation and apoptosis. NOD2/CARD15 is a PRM that recognizes specific peptidoglycan (PGN) components of bacterial cell walls (reviewed in Strober et al. 2006, and Inohara et al. 2003). NOD2 is expressed in cells that are exposed to PGN under physiological conditions including antigen-presenting cells (APCs) such as macrophages and dendritic cells, and epithelial cells (reviewed in Strober et al. 2006). NOD2 is a for thought to play a role in the pathogenesis of human gastrointestinal disease. Polymorphisms and mutations in NOD2 are associated with susceptibility to Crohn's disease, a chronic inflammatory bowel disease (Leung et al., 2006). Additionally, polymorphisms in NOD2 have also been found to be associated with Graft-versus-Host Disease (GVHD) (Holler et al., 2004)This association of NOD2 gene polymorphisms with gastrointestinal disease and GVHD suggests that NOD2 gene polymorphisms play a role in pro-inflammatory diseases. NBP2-27328 recognizes NOD2, a 1040 amino acid protein. NBP2-27328 also recognizes NOD2 alternatively spliced and mutated forms which contain the peptide immunogen sequence including NOD2 short, a 273 amino acid protein.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.