NOD2 Antibody - BSA Free

• Reactivity: Hu, Mu, Pm, Pm
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NOD2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NOD2 Antibody - BSA Free (NBP2-27118) is a polyclonal antibody validated for use in WB and IP. Anti-NOD2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a synthetic peptide corresponding to amino acids 125-162 of human Nod2.
• Specificity: Researchers are encouraged to optimize dilutions for their specific testing, and to use the sequence information and accession number (NP_071445.1/Q9HC29) that was used to design the peptide immunogen in order to assess potential cross-reactivity of the antibody.
• Predicted Species: Primate (97%), Monkey (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOD2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

The amino acid sequence used as immunogen is 95% homologous in Lar gibbon (Hylobates lar), 97% in chimpanzee.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole sera with 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for NOD2 Antibody - BSA Free

• BLAU
• CARD15caspase recruitment domain protein 15
• caspase recruitment domain family, member 15
• Caspase recruitment domain-containing protein 15
• CDACUG
• CLR16.3
• IBD1NLR family, CARD domain containing 2
• Inflammatory bowel disease protein 1
• NLRC2
• NOD-like receptor C2
• nucleotide-binding oligomerization domain 2
• nucleotide-binding oligomerization domain containing 2
• nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
• nucleotide-binding oligomerization domain-containing protein 2
• PSORAS1NOD2B

Background

Apaf-1 and Nod1 are members of a protein family, each of which contains a caspase recruitment domain (CARD) linked to a nucleotide-binding domain, which regulate apoptosis and/or NF-kB activation. Recently, Nod2, a third member of the family has been identified. Recent studies indicate that Nod2 activates NF-kB, but unlike Nod1, this new homologue is primary expressed in monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NOD2 Antibody (NBP2-27118)(1)

Publication using NBP2-27118

• Chauhan VS, Marriott I. Differential roles for NOD2 in osteoblast inflammatory immune responses to bacterial pathogens of bone tissue. J Med Microbiol. 2010-07-01 [PMID: 20360399] (IP, Mouse)
  
  Details:
  1. IP (mouse thymus and primary mouse osteoblast cell cultures), Fig 6a. Immunoprecipitates were western blotted with an antibody to Rip2. The results showed the IMX-5053 antibody co-IP'd Rip2.

FAQs for NOD2 Antibody (NBP2-27118). (Showing 1 - 1 of 1 FAQ).

1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
   • For detecting endogenous NOD2 in human samples, I would recommend NB100-524. This antibody has been validated for use in WB, IHC-Paraffin and IP.

Bioinformatics

• Gene Symbol: NOD2
• Uniprot:
  • Q9HC29(Human)