NLRP3/NALP3 Cell Lysate

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Western Blot: NLRP3/NALP3 Cell Lysate [NBP2-27589] - 10 ul of A) human NALP3 transfected cell lysate (NBP2-27589), B) mouse NALP3 transfected cell lysate (NBP2-29320) and C) untransfected cell lysate were probed with 3 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Concentration
2.0 mg/ml

Order Details

NLRP3/NALP3 Cell Lysate Summary

Immunogen
50ul each of Human NALP3 Transfected and Untransfected Cell Lysate
Gene
NLRP3

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
HEK293 cells were transfected with full-length human NALP3. Load 10 ul/lane of a 12 well mini-gel. This transfected cell lysate can be used as a positive western blot control.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1 x SDS sample buffer 60 mM Tris-Hcl (pH 6.8) 10 mM EDTA 2% SDS 0.03% Bromophenol Blue 10% glycerol 0.3% Beta-mercaptoethanol
Concentration
2.0 mg/ml

Lysate Details for Array

Type
Cell

Alternate Names for NLRP3/NALP3 Cell Lysate

  • AGTAVPRL
  • AII
  • AII/AVP
  • Angiotensin/vasopressin receptor AII/AVP-like
  • AVP
  • C1orf7
  • Caterpiller protein 1.1
  • CIAS1
  • CLR1.1
  • Cold autoinflammatory syndrome 1 protein
  • Cold autoinflammatory syndrome 1
  • Cryopyrin
  • Familial cold autoinflammatory syndrome
  • FCAS
  • FCU
  • Leucine-rich repeat-, and PYD-containing protein 3
  • Muckle-Wells syndrome
  • MWS
  • NACHT
  • NACHT, LRR and PYD containing protein 3
  • NACHT, LRR and PYD domains-containing protein 3
  • NALP3
  • NLR family, pyrin domain containing 3
  • NLRP3
  • Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
  • PYPAF1
  • PYRIN-containing APAF1-like protein 1

Background

NACHT, LRR and PYD domains-containing protein 3 belongs to the NLRP family and it contains one DAPIN domain, seven LRR (leucine-rich) repeats and one NATCH domain. It interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling and it plays a role in the regulation of inflammation, the immune response and apoptosis. It is also reported that the NLRP3 inflammasome can be activated by pathogen-associated molecular patterns (PAMPs) or endogenous danger-associated molecular patterns (DAMPs). The activation of the NLRP3 inflammasome results in proteolytic activation and secretion of cytokines of the interleukin-1 (IL-1) family. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle- Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome and neonatal-onset multi-system inflammatory disease (NOMID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NLRP3/NALP3 Lysate (NBP2-27589). (Showing 1 - 4 of 4 FAQ).

  1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
    • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
  2. Does NLRP3/NALP3 antibodies come in lyophilized form?
    • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
  3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
    • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
  4. The customer wants to know if the concentration information is available for this product.
    • The lab told me that is at the concentration of 2.0 ug/ul.

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Bioinformatics Tool for NLRP3/NALP3 Lysate (NBP2-27589)

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Diseases for NLRP3/NALP3 Lysate (NBP2-27589)

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Pathways for NLRP3/NALP3 Lysate (NBP2-27589)

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PTMs for NLRP3/NALP3 Lysate (NBP2-27589)

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Blogs on NLRP3/NALP3.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

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Bioinformatics

Gene Symbol NLRP3