NLRP3/NALP3 Antibody (8N8E9)

Images

 
Western Blot: NLRP3/NALP3 Antibody (8N8E9) [NBP2-03947] - Analysis of extracts from mouse spleen and Jurkat cells using NALP3 antibody (NBP2-03947) at 1:100.
Western Blot: NLRP3/NALP3 Antibody (8N8E9) [NBP2-03947] - Analysis using NALP3 monoclonal antibody. (A), (B) and the recombinant human NALP3 Pyrin doman protein immunogen (C) probed with 5 ug/ml (tranfected) or 0.5 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
8N8E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

NLRP3/NALP3 Antibody (8N8E9) Summary

Immunogen
A recombinant protein corresponding to the Pyrin domain (PYD) of human NALP3 (amino acids 6-89) was used as the immunogen for this antibody. NALP3 belongs to a class of protein motifs known as the death fold. As a class, death fold motifs primarily function in regulating inflammatory and apoptosis responses. The PYD of NALP3 is required for association with ACS during inflammasome formation.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NLRP3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-03947.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NLRP3/NALP3 Antibody (8N8E9)

  • AGTAVPRL
  • AII
  • AII/AVP
  • Angiotensin/vasopressin receptor AII/AVP-like
  • AVP
  • C1orf7
  • Caterpiller protein 1.1
  • CIAS1
  • CLR1.1
  • Cold autoinflammatory syndrome 1 protein
  • Cold autoinflammatory syndrome 1
  • Cryopyrin
  • Familial cold autoinflammatory syndrome
  • FCAS
  • FCU
  • Leucine-rich repeat-, and PYD-containing protein 3
  • Muckle-Wells syndrome
  • MWS
  • NACHT
  • NACHT, LRR and PYD containing protein 3
  • NACHT, LRR and PYD domains-containing protein 3
  • NALP3
  • NLR family, pyrin domain containing 3
  • NLRP3
  • Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
  • PYPAF1
  • PYRIN-containing APAF1-like protein 1

Background

NLRP3 belongs to the NLR [nucleotide binding oligomerization domain (NOD) and leucine rich region repeat (LRR) containing proteins] family of innate immune cytoplasmic receptors (reviewed in De Nardo and Latz, 2011; Kanneganti, 2010; Anand, 2011). NLR family members contain an N-terminus effector region consisting of either a pyrin (PYD), a caspase activation and recruitment (CARD), or a baculorvirus inhibitor of apoptosis protein repeat (BIR) domain. NLRs also have an intermediate conserved NOD domain and a C-terminus ligand binding LRR domain. NLRs, like TLRs (Toll-like receptors), alert the mammalian immune system to the presence of PAMPs (pathogen associated molecular patterns) and DAMPs (danger associated molecular patterns). Like a number of other NLRs, NLRP3 functions in a multiprotein complex called the inflammasome. The inclusion of a particular NLR within an inflammasome depends upon the upstream trigger. The NLRP3 inflammasome is considered to be a global sensor and assembles in response to a broad spectrum of PAMPs and DAMPs. The PYD of activated NLRP3 associates with the PYD of ASC, triggering the CARD of ASC to associate with the CARD of caspase-1 and completes the assembly of the NLRP3 inflammasome. The inflammasome serves as a platform for caspase-1 activation. Active caspase-1 cleaves the proforms of the proinflammatory cytokines IL-1beta, IL-18, and IL-33 to their active, secreted forms. The mechanisms from NLRP3 activation to inflammasome formation and caspase-1 activation remain to fully elucidated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NLRP3/NALP3 Antibody (NBP2-03947)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NLRP3/NALP3 Antibody (NBP2-03947). (Showing 1 - 4 of 4 FAQ).

  1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
    • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
  2. Does NLRP3/NALP3 antibodies come in lyophilized form?
    • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
  3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
    • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
  4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
    • A recombinant sequence of amino acids 6 - 89 within the Pyrin domain (PYD) of human NLRP3/NALP3.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 700 Labeled NBP2-03947AF700
Biotin Labeled NBP2-03947B
DyLight 488 Labeled NBP2-03947G
DyLight 550 Labeled NBP2-03947R
DyLight 650 Labeled NBP2-03947C
DyLight 680 Labeled NBP2-03947FR
DyLight 755 Labeled NBP2-03947IR
HRP Labeled NBP2-03947H

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Blogs on NLRP3/NALP3.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

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Bioinformatics

Gene Symbol NLRP3
Entrez
Uniprot