NLRP1/NALP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56147] - FFPE normal human bone marrow stained for NALP1 (CARD7/NAP) using NB100-56147 at 1:2000. Hematoxylin-eosin counterstain. Staining is seen in differentiated granulocytes.

Immunoprecipitation: NLRP1/NALP1 Antibody [NB100-56147] - Analysis of NLRP1/NALP1(NAC/CARD7) in Jurkat cells using NB100-56148 and NB100-56147 NALP1 antibodies. Cell lysates were immunoprecipitated with NB100-56148 (2 uL/million cells) and Western blotted with NB100-56147 at 1:2000.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NLRP1/NALP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NLRP1/NALP1 Antibody - BSA Free (NB100-56147) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-NLRP1/NALP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to amino acids 1058-1077 (CVPSPASQGDLHTKPLGTDD) of human NALP1 (NAC/CARD7) was used as immunogen, GenBank no. NP_127497.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 1:50 - 1:200
  • Western Blot 1:1000 - 1:2000
• Control:

  NLRP1/NALP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for NLRP1/NALP1 Antibody - BSA Free

• CARD7
• CARD7SLEV1
• Caspase recruitment domain-containing protein 7
• CLR17.1
• Death effector filament-forming ced-4-like apoptosis protein
• DEFCAP
• DEFCAPVAMAS1
• DKFZp586O1822
• KIAA0926DEFCAP-L/S
• NACHT, leucine rich repeat and PYD (pyrin domain) containing 1
• NACHT, leucine rich repeat and PYD containing 1
• NACHT, LRR and PYD containing protein 1
• NACHT, LRR and PYD domains-containing protein 1
• NACPP1044
• NALP1
• NALP1caspase recruitment domain protein 7
• NLR family, pyrin domain containing 1
• NLRP1
• Nucleotide-binding domain and caspase recruitment domain
• nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 1
• PP1044
• SLEV1
• VAMAS1

Background

NALP1 (also known as NAC/CARD7) is a member of the CATERPILLER (CLR) diverse multigene superfamily of immune regulatory proteins (Ting and Davis, 2005). CLR proteins have a variable N-terminal domain, followed by a nucleotide-binding domain (NBD) and leucine-rich repeats (LRR). The N-terminal domain consists of transactivation, CARD, Pyrin or BIR domains, or in some cases is undefined. The CLR family, also known as the NOD family, has its ancient roots in the plant kingdom and is related to the disease resistance (R) gene family that mediates plant immune responses. CLR proteins participate in both innate and adaptive immunity in mammals, some act as sensors that detect pathogen products, and several CLR genes have been linked to susceptibility to immunological diseases. It is thought that the CLR family may have a fundamental importance for the function of the mammalian innate/ancient immune system on par with the Toll-like receptor (TLR) family. NALP1, a member of NALP CLR subfamily, has an N-terminal pyrin domain (PYD), followed by an NBD, LRR, and a C-terminal CARD domain (reviewed in Tschopp. NALP1 is a component of both the inflammasome and apoptosome, suggesting that NALP1 it has important roles in both inflammation and apoptosis. This antibody recognizes NALP1; human NALP1 is a 1473 amino acid protein and migrates at approx. 166 kDa on SDS-PAGE. However, multiple splice variants encoding distinct NALP1 isoforms with varying amino acid lengths have been described and thus the molecular weight detected may vary depending on the isoform(s) expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NLRP1/NALP1 Antibody (NB100-56147)(1)

Publication using NB100-56147

• Xu H, Akinyemi IA, Chitre SA et al. SARS-CoV-2 viroporin encoded by ORF3a triggers the NLRP3 inflammatory pathway Virology 2022-01-17 [PMID: 35066302] (WB)

Bioinformatics

• Gene Symbol: NLRP1
• Entrez:
  • 22861(Human)