PARP Antibody (194C1439) - Cleaved


Biological Strategies: Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band more
Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. more
Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.

Product Details

Reactivity Hu, Mu, Rt(-)Species Glossary
Applications WB, Flow, Flow-IC, ICC/IF
1.0 mg/ml
Validated by:

Biological Strategies


Order Details

PARP Antibody (194C1439) - Cleaved Summary

This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP.
This monoclonal antibody specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.
IgG2b Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 - 2 ug/mL
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
  • Flow (Intracellular) 1 - 10 ug/1x10^6 cells
PARP Knockout 293T Cell Lysate
Read Publications using
NB100-56599 in the following applications:

  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29622764). Rat negative reported via customer review.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for PARP Antibody (194C1439) - Cleaved

  • ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
  • ADPRT 1
  • ADPRTADP-ribosyltransferase NAD(+)
  • cleaved PARP 1
  • cleaved PARP apoptosis
  • cleaved PARP western blot
  • cleaved PARP
  • cleaved PARP-1
  • EC 2.4.2
  • EC
  • NAD(+) ADP-ribosyltransferase 1
  • PARP apoptosis
  • PARP
  • PARP1
  • PARP-1
  • poly (ADP-ribose) polymerase 1
  • poly (ADP-ribose) polymerase family, member 1
  • poly [ADP-ribose] polymerase 1
  • poly(ADP-ribose) polymerase
  • poly(ADP-ribose) synthetase
  • poly(ADP-ribosyl)transferase
  • Poly[ADP-ribose] synthase 1
  • PPOL


The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Ma
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Species: Hu, Mu, Rt
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Species: Hu, Mu, Rt
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Species: Hu, Mu, Rt
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Species: Hu, Rt
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Species: Hu, Mu, Rt, Pm
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Applications: WB, Simple Western, IP
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Publications for PARP Antibody (NB100-56599)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 7 of 7.
Publications using NB100-56599 Applications Species
Mega Tiber P, Kocyigit Sevinc S, Kilinc O, Orun O Biological effects of whole Z.Officinale extract on chronic myeloid leukemia cell line K562. Gene. 2019 Apr 15 [PMID: 30684525] (WB, Human)

Citation using the Azide free version of this antibody.
WB Human
Dou X, Li S, Hu L et al. Glutathione disulfide sensitizes hepatocytes to TNFa-mediated cytotoxicity via IKK-b S-glutathionylation: a potential mechanism underlying non-alcoholic fatty liver disease. Exp. Mol. Med. Apr 6 2018 [PMID: 29622764] (WB, Mouse) WB Mouse
Melao A, Spit M, Cardoso BA, Barata JT Optimal interleukin-7 receptor-mediated signaling, cell cycle progression and viability of T-cell acute lymphoblastic leukemia cells rely on casein kinase 2 activity. Haematologica. 2016 Nov [PMID: 27470599] (WB)

Citation using the Azide Free version of this antibody.
Kamal A, Sreekanth K, Kumar PP et al. Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines. Eur J Med Chem. 2010 Jun [PMID: 20171761]

Products cited for western blot: 1. PARP (IMG-401A): MCF-7 cells treated with numerous compounds, Fig 7. 2. B-Tubulin (IMG-5180A): MCF-7 cells treated with PP-PBDs, Fig 6 and Fig 7. 3. Cytochrome C (IMG-101A): MCF-7 cells treated with numerous compounds,
Ko CH, Shen SC, Chen YC. Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species. Free Radic Biol Med. 2004 Apr 1 [PMID: 15019974]
Chen YC, Shen SC, Lin HY. Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids. Biochem Pharmacol. 2003 Oct 1 [PMID: 14505793]
Chen YC, Shen SC, Lee WR et al. Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production. Biochem Pharmacol. 2002 Dec 15 [PMID: 12445860]

Reviews for PARP Antibody (NB100-56599) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PARP Antibody (NB100-56599). (Showing 1 - 2 of 2 FAQs).

  1. I was wondering if you would mind letting me know which product would you recommend for immunochemistry of the retina of the rat and mouse by poly (ADP-ribose) polymerase (PARP) and calpain. There are many different calpain antibodies in your website. The samples are 12-um vertical cryostst sections (fixed by PFA).
    • For PARP, I would recommend either NB120-2168 or NB100-64828. Bear in mind that they are both mouse monoclonals, and you may have to take extra steps to reduce mouse-on-mouse background.
  2. Do you have any 100 coda or bigger controls?
    • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-27335AF350
Alexa Fluor 405 NBP2-27335AF405
Alexa Fluor 488 NBP2-27335AF488
Alexa Fluor 532 NBP2-27335AF532
Alexa Fluor 594 NBP2-27335AF594
Alexa Fluor 647 NBP2-27335AF647
Alexa Fluor 700 NBP2-27335AF700
Alexa Fluor 750 NBP2-27335AF750
Allophycocyanin NBP2-27335APC
Biotin NBP2-27335B
DyLight 350 NBP2-27335UV
DyLight 405 NBP2-27335V
DyLight 488 NBP2-27335G
DyLight 550 NBP2-27335R
DyLight 594 NBP2-27335DL594
DyLight 650 NBP2-27335C
DyLight 680 NBP2-27335FR
DyLight 755 NBP2-27335IR
FITC NBP2-27335F
HRP NBP2-27335H
Janelia Fluor 549 NBP2-27335JF549
Janelia Fluor 646 NBP2-27335JF646
PE NBP2-27335PE
PerCP NBP2-27335PCP

Additional PARP Products

Array NB100-56599

Bioinformatics Tool for PARP Antibody (NB100-56599)

Discover related pathways, diseases and genes to PARP Antibody (NB100-56599). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for PARP Antibody (NB100-56599)

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Blogs on PARP.

What are the major differences between Apoptosis, Necroptosis & Autophagy?
Apoptosis is a form of programmed cell death which is mediated by cysteine proteases called caspases. It is an essential phenomenon in the maintenance of homeostasis and growth of tissues, and it also plays a critical role in immune response. The ...  Read full blog post.

The role of PARP-1 in the repair of single stranded break (SSB)
PARPs (poly ADP ribose polymerases) are DNA repair enzymes that promote single stranded break (SSB) repair by binding to DNA at the sites of SSBs and recruiting repair machinery. In humans, the PARP superfamily consists of 17 members, of which five...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 7: The Cell's Suicide Switch
Caspase 7 (also known as CASP7, Mch3, ICE-LAP3, CMH-1) is a member of caspase family of cysteine proteases. It is an apoptosis-related cystein peptidase encoded by the CASP7 gene in humans. CASP7 homologous sequences have been identified in nearly all...  Read full blog post.

PARP Antibody Assays aid both Apoptosis and Cancer Research
The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play...  Read full blog post.

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Gene Symbol PARP1