PARP Antibody (194C1439) - Cleaved - BSA Free

Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.

Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.

• Reactivity: Hu, Mu, Rt(-)
• Applications: WB, Flow, ICC/IF
• Clone: 194C1439
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PARP Antibody (194C1439) - Cleaved - BSA Free Summary

• Immunogen: This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP.
• Specificity: This monoclonal antibody specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PARP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) 1 - 10 ug/1x10^6 cells
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
  • Western Blot 1 - 2 ug/mL

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29622764). Rat negative reported via customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PARP Antibody (194C1439) - Cleaved - BSA Free

• ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
• ADPRT 1
• ADPRT
• ADPRTADP-ribosyltransferase NAD(+)
• cleaved PARP 1
• cleaved PARP apoptosis
• cleaved PARP western blot
• cleaved PARP
• cleaved PARP-1
• EC 2.4.2
• EC 2.4.2.30
• NAD(+) ADP-ribosyltransferase 1
• PARP apoptosis
• PARP
• PARP1
• PARP-1
• PARPADPRT1
• poly (ADP-ribose) polymerase 1
• poly (ADP-ribose) polymerase family, member 1
• poly [ADP-ribose] polymerase 1
• poly(ADP-ribose) polymerase
• poly(ADP-ribose) synthetase
• poly(ADP-ribosyl)transferase
• Poly[ADP-ribose] synthase 1
• PPOL
• PPOLpADPRT-1

Background

The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PARP Antibody (NB100-56599)(9)

Publications using NB100-56599

• Szabó V, Csákány-Papp N, Görög M et al. Orai1 calcium channel inhibition prevents progression of chronic pancreatitis JCI insight 2023-05-25 [PMID: 37227782] (IHC-P, Human, Mouse)
  
  Details:
  Dilution 1:200
• Erdogan S, Turkekul K Neferine inhibits proliferation and migration of human prostate cancer stem cells through p38 MAPK/JNK activation Food Biochem 2020-05-11 [PMID: 32394497] (WB, Human)
  
  Details:
  Citation using the Azide Free format of this antibody.
• Mega Tiber P, Kocyigit Sevinc S, Kilinc O, Orun O Biological effects of whole Z.Officinale extract on chronic myeloid leukemia cell line K562. Gene. 2019-04-15 [PMID: 30684525] (WB, Human)
  
  Details:
  Citation using the Azide free version of this antibody.
• Dou X, Li S, Hu L et al. Glutathione disulfide sensitizes hepatocytes to TNFa-mediated cytotoxicity via IKK-b S-glutathionylation: a potential mechanism underlying non-alcoholic fatty liver disease. Exp. Mol. Med. 2018-04-06 [PMID: 29622764] (WB, Mouse)
• Melao A, Spit M, Cardoso BA, Barata JT Optimal interleukin-7 receptor-mediated signaling, cell cycle progression and viability of T-cell acute lymphoblastic leukemia cells rely on casein kinase 2 activity. Haematologica. 2016-11-01 [PMID: 27470599] (WB)
  
  Details:
  Citation using the Azide Free version of this antibody.
• Kamal A, Sreekanth K, Kumar PP et al. Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines. Eur J Med Chem. 2010-06-01 [PMID: 20171761]
  
  Details:
  Products cited for western blot: 1. PARP (IMG-401A): MCF-7 cells treated with numerous compounds, Fig 7. 2. B-Tubulin (IMG-5180A): MCF-7 cells treated with PP-PBDs, Fig 6 and Fig 7. 3. Cytochrome C (IMG-101A): MCF-7 cells treated with numerous compounds,
• Ko CH, Shen SC, Chen YC. Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species. Free Radic Biol Med. 2004-04-01 [PMID: 15019974]
• Chen YC, Shen SC, Lin HY. Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids. Biochem Pharmacol. 2003-10-01 [PMID: 14505793]
• Chen YC, Shen SC, Lee WR et al. Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production. Biochem Pharmacol. 2002-12-15 [PMID: 12445860]

FAQs for PARP Antibody (NB100-56599). (Showing 1 - 2 of 2 FAQs).

1. I was wondering if you would mind letting me know which product would you recommend for immunochemistry of the retina of the rat and mouse by poly (ADP-ribose) polymerase (PARP) and calpain. There are many different calpain antibodies in your website. The samples are 12-um vertical cryostst sections (fixed by PFA).
   • For PARP, I would recommend either NB120-2168 or NB100-64828. Bear in mind that they are both mouse monoclonals, and you may have to take extra steps to reduce mouse-on-mouse background.
2. Do you have any 100 coda or bigger controls?
   • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Bioinformatics

• Gene Symbol: PARP1