NKX2.2 Antibody (3E4) - BSA Free

Western Blot: NKX2.2 Antibody (3E4) [NBP2-37486] - Western blot analysis using NKX2.2 mAb against human NKX2.2 recombinant protein.(Expected MW is 22 kDa)

Flow Cytometry: NKX2.2 Antibody (3E4) [NBP2-37486] - Flow cytometric analysis of MCF-7 cells using NKX2.2 mouse mAb (green) and negative control (red).

ELISA: NKX2.2 Antibody (3E4) [NBP2-37486] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 3E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NKX2.2 Antibody (3E4) - BSA Free Summary

• Description: Novus Biologicals Mouse NKX2.2 Antibody (3E4) - BSA Free (NBP2-37486) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human NKX2.2 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NKX2-2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 30.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for NKX2.2 Antibody (3E4) - BSA Free

• Homeobox protein NK-2 homolog B
• NK-2 (Drosophila) homolog B
• NK2 homeobox 2
• NK2 transcription factor related, locus 2 (Drosophila)
• NK2 transcription factor related, locus 2
• NK2 transcription factor-like protein B
• NKX2.2
• NKX2.2homeobox protein Nkx-2.2
• NKX2B
• NKX2BNK-2 homolog B

Background

Nkx2.2, or Homeobox protein Nkx-2.2, consists of a 273 amino acid isoform that is 30 kDa, and is involved in controlling and maintaining the expression of genes within pancreatic endocrine cells and axons. Current research is being conducted on the relationship between Nkx2.2 and a variety of diseases and disorders, including glioma, neuronitis, maturity-onset diabetes, pancreatitis, Partington syndrome, sarcoma, gastrointestinal neuroendocrine tumor, acute lymphoblastic leukemia, multiple sclerosis, and carcinoid tumors. The protein has been linked to the Wnt, Hedgehog, and notch pathways, as well as regulation of beta-cell development and gene expression. NKx2.2 interacts with several other proteins, including OLIG2, HDAC1, SIN3A, FOXA2, and FOXO1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NKX2-2