NHE8 Antibody (7A11) - BSA Free

Western Blot: NHE8 Antibody (7A11) [NB110-62091] - Detection of NHE8 (7A11) in HeLa whole cell lysate

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 7A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.2 mg/ml

NHE8 Antibody (7A11) - BSA Free Summary

• Description: Novus Biologicals Mouse NHE8 Antibody (7A11) - BSA Free (NB110-62091) is a monoclonal antibody validated for use in WB and ICC/IF. Anti-NHE8 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A fusion protein containing the C-terminal 89 residues of human NHE8. [Swiss-Prot# Q9Y2E8]
• Localization: Golgi apparatus membrane; multi-pass membrane.
• Specificity: This is specific for the 85 kDa mature glycosylated form of the protein. It does not recognize the 55 kDa immature form.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC9A8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 2 ug/ml (ECL)
• Application Notes: This NHE8 antibody is useful for Western blot analysis where a band is seen at ~80 kDa. Please note that these samples should not be boiled. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NHE8 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.2 mg/ml
• Purity: Protein G purified

Alternate Names for NHE8 Antibody (7A11) - BSA Free

• DKFZp686C03237
• KIAA0939
• KIAA0939DKFZp686C03237
• MGC138418
• Na(+)/H(+) exchanger 8
• NHE8
• NHE-8
• NHE8FLJ42500
• SLC9A8
• sodium/hydrogen exchanger 8
• solute carrier family 9 (sodium/hydrogen exchanger)
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 8
• solute carrier family 9 (sodium/hydrogen exchanger), member 8
• Solute carrier family 9 member 8

Background

NHE (Na+/H+ Exchanger) family members are integral membrane proteins which have been identified to play a key role in catalyzing the extrusion of intracellular proton (H+) ions in exchange for extracellular sodium (Na+) ions for regulation of cellular pH as well as transepithelial ion/water transport. NHE8 is a relatively new member of Na+/H+ exchanger family and is a ubiquitously expressed protein with highest levels detected in epithelial cells of the intestine and the kidney as well as in skeletal muscles. Interestingly, the intestinal/renal expression levels of NHE8 are significantly higher than that of NHE2/NHE3 at a young age, suggesting that NHE8 as an important player for sodium absorption during early development. Localized mainly in Golgi apparatus membranes as multi-pass membrane protein, NHE8 is implicated in regulation of pH to eliminate acids generated by active metabolism or to counter adverse environmental conditions. NHE8 is a major proton extruding system driven by the inward sodium ion chemical gradient and has also been proposed to play role in signaling mechanisms also.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NHE8 Antibody (NB110-62091)(5)

Publications using NB110-62091

• Kinaneh S, Knany Y, Khoury EE et al. Identification, localization and expression of NHE isoforms in the alveolar epithelial cells PLOS ONE 2021-04-21 [PMID: 33882062] (Western Blot, Immunocytochemistry/ Immunofluorescence, Rat)
• Baum M, Martin MG, Booth IW, Holmberg C, Twombley K, Zhang Q, Gattineni J, Moe O. Nucleotide sequence of the Na+/H+ exchanger-8 in patients with congenital sodium diarrhea. J Pediatr Gastroenterol Nutr;53(5):474-7. 2011-11-01 [PMID: 21666503] (WB, Mouse)
• Twombley K, Gattineni J, Bobulescu IA, Dwarakanath V, Baum M. Effect of metabolic acidosis on neonatal proximal tubule acidification. Am J Physiol Regul Integr Comp Physiol;299(5):R1360-8. 2010-11-01 [PMID: 20720175] (WB, Rat, Mouse)
• Zhang J, Bobulescu IA, Goyal S, Aronson PS, Baum MG, Moe OW. Characterization of Na+/H+ exchanger NHE8 in cultured renal epithelial cells. Am J Physiol Renal Physiol;293(3):F761-6. 2007-09-01 [PMID: 17581925]
• Goyal, S et al. Immunolocalization of NHE8 in rat kidney. Am J Physiol Renal Physiol;288(3):F530-8. 2005-03-01 [PMID: 15522984] (WB, ICC/IF, Rat)

Bioinformatics

• Gene Symbol: SLC9A8