NHE1/SLC9A1 Antibody

Images

 
Western Blot: NHE1/SLC9A1 Antibody [NBP1-76847] - Rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: NHE1/SLC9A1 Antibody [NBP1-76847] - Immunofluorescence of Nhe-1 in Human Brain tissue with Nhe-1 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP1-76847] - Human brain tissue using Nhe-1 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

NHE1/SLC9A1 Antibody Summary

Immunogen
Antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Nhe-1. The immunogen is located within the last 50 amino acids of Nhe-1. Amino Acid Squence: CLSDPGPHPEPGEGEPFFPK
Specificity
At least three isoforms of Nhe-1 are known to exist.
Predicted Species
Porcine (95%), Bovine (95%), Rabbit (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC9A1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2-5 ug/ml
  • Immunohistochemistry-Paraffin 2-5 ug/ml
Theoretical MW
50, 90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
NHE1/SLC9A1 Peptide (NBP1-76847PEP)
Publications
Read Publication using
NBP1-76847 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for NHE1/SLC9A1 Antibody

  • APNH
  • APNH1
  • APNHFLJ42224
  • Na(+)/H(+) antiporter, amiloride-sensitive
  • Na(+)/H(+) exchanger 1
  • Na+/H+, amiloride sensitive)
  • Na-Li countertransporter
  • NHE1
  • NHE-1
  • NHE1Na+/H+ antiporter, amiloride-sensitive
  • SLC9A1
  • sodium/hydrogen exchanger 1
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter
  • solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter
  • solute carrier family 9 (sodium/hydrogen exchanger), member 1
  • Solute carrier family 9 member 1

Background

The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NHE1/SLC9A1 Antibody (NBP1-76847)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NHE1/SLC9A1 Antibody (NBP1-76847). (Showing 1 - 1 of 1 FAQ).

  1. I would like to know if the NHE1 antibody binds to intracellular compartments that may have NHE1 (I.e. the mitochondria) or if they only bind to the plasma membrane NHE1
    • The NHE1 Antibody (NBP1-76847) was raised against a 20 amino acid peptide near the carboxyl terminus of the human Nhe-1 protein. From uniprot we can see in subcelluar location that AAs 500-815 correspond to the topological domain for the cytoplasmic region where this epitope should be located however we do not know of any specific interaction with organelles within the cytoplasm such as the mitochondria however uniprot does mention the Endoplasmic Reticulum membrane. http://www.uniprot.org/uniprot/P19634#subcellular_location

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Bioinformatics

Gene Symbol SLC9A1
Entrez
Uniprot