Antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Nhe-1. The immunogen is located within the last 50 amino acids of Nhe-1. Amino Acid Squence: CLSDPGPHPEPGEGEPFFPK
At least three isoforms of Nhe-1 are known to exist.
50, 90 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for NHE1/SLC9A1 Antibody
Na(+)/H(+) antiporter, amiloride-sensitive
Na(+)/H(+) exchanger 1
Na+/H+, amiloride sensitive)
NHE1Na+/H+ antiporter, amiloride-sensitive
sodium/hydrogen exchanger 1
solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter
solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter
solute carrier family 9 (sodium/hydrogen exchanger), member 1
Solute carrier family 9 member 1
The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for NHE1/SLC9A1 Antibody (NBP1-76847). (Showing 1 - 1 of 1 FAQ).
I would like to know if the NHE1 antibody binds to intracellular compartments that may have NHE1 (I.e. the mitochondria) or if they only bind to the plasma membrane NHE1
The NHE1 Antibody (NBP1-76847) was raised against a 20 amino acid peptide near the carboxyl terminus of the human Nhe-1 protein. From uniprot we can see in subcelluar location that AAs 500-815 correspond to the topological domain for the cytoplasmic region where this epitope should be located however we do not know of any specific interaction with organelles within the cytoplasm such as the mitochondria however uniprot does mention the Endoplasmic Reticulum membrane. http://www.uniprot.org/uniprot/P19634#subcellular_location
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