NHE8 Antibody (7A11) [Allophycocyanin]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 7A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

NHE8 Antibody (7A11) [Allophycocyanin] Summary

• Immunogen: A fusion protein containing the C-terminal 89 residues of human NHE8. [Swiss-Prot# Q9Y2E8]
• Localization: Golgi apparatus membrane; multi-pass membrane.
• Specificity: This is specific for the 85 kDa mature glycosylated form of the protein. It does not recognize the 55 kDa immature form.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC9A8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This NHE8 antibody is useful for Western blot analysis where a band is seen at ~80 kDa. Please note that these samples should not be boiled.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for NHE8 Antibody (7A11) [Allophycocyanin]

• DKFZp686C03237
• KIAA0939
• KIAA0939DKFZp686C03237
• MGC138418
• Na(+)/H(+) exchanger 8
• NHE8
• NHE-8
• NHE8FLJ42500
• SLC9A8
• sodium/hydrogen exchanger 8
• solute carrier family 9 (sodium/hydrogen exchanger)
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 8
• solute carrier family 9 (sodium/hydrogen exchanger), member 8
• Solute carrier family 9 member 8

Background

NHE8, also known as SLC9A8, is an integral transmembrane proteins that exchange extracellular Na+ for intracellular H+. NHE8 is expressed in the kidney, where it may contribute to apical membrane ion transport physiology. NHE8 is highly expressed in the proximal tubules in the outer stripe of the outer medulla with significant but lower expression in the proximal tubules in the cortex. Sodium-Hydrogen ion Exchangers (NHEs) are a growing family of transmembrane proteins, and NHE8 antibodies are useful tools for research on NaH ion exchange.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC9A8