NHE8 Antibody (7A11) [FITC]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 7A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

NHE8 Antibody (7A11) [FITC] Summary

• Immunogen: A fusion protein containing the C-terminal 89 residues of human NHE8. [Swiss-Prot# Q9Y2E8]
• Localization: Golgi apparatus membrane; multi-pass membrane.
• Specificity: This is specific for the 85 kDa mature glycosylated form of the protein. It does not recognize the 55 kDa immature form.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC9A8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for NHE8 Antibody (7A11) [FITC]

• DKFZp686C03237
• KIAA0939
• KIAA0939DKFZp686C03237
• MGC138418
• Na(+)/H(+) exchanger 8
• NHE8
• NHE-8
• NHE8FLJ42500
• SLC9A8
• sodium/hydrogen exchanger 8
• solute carrier family 9 (sodium/hydrogen exchanger)
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 8
• solute carrier family 9 (sodium/hydrogen exchanger), member 8
• Solute carrier family 9 member 8

Background

NHE (Na+/H+ Exchanger) family members are integral membrane proteins which have been identified to play a key role in catalyzing the extrusion of intracellular proton (H+) ions in exchange for extracellular sodium (Na+) ions for regulation of cellular pH as well as transepithelial ion/water transport. NHE8 is a relatively new member of Na+/H+ exchanger family and is a ubiquitously expressed protein with highest levels detected in epithelial cells of the intestine and the kidney as well as in skeletal muscles. Interestingly, the intestinal/renal expression levels of NHE8 are significantly higher than that of NHE2/NHE3 at a young age, suggesting that NHE8 as an important player for sodium absorption during early development. Localized mainly in Golgi apparatus membranes as multi-pass membrane protein, NHE8 is implicated in regulation of pH to eliminate acids generated by active metabolism or to counter adverse environmental conditions. NHE8 is a major proton extruding system driven by the inward sodium ion chemical gradient and has also been proposed to play role in signaling mechanisms also.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC9A8