NF-H Antibody (NF-01)

Images

 
Immunocytochemistry/ Immunofluorescence: NF-H Antibody (NF-01) [NB500-416] - Analysis using the Alexa Fluor (R) 488 conjugate of NB500-416. Ataining of NF-H in frozen monkey foot pad skin shows sensory fibers. Image ...read more
Immunohistochemistry-Paraffin: NF-H Antibody (NF-01) [NB500-416] - Human cerebellum (paraffin-embedded sections) with anti-200kDa Neurofilament Heavy protein (NF-01).
Immunohistochemistry-Paraffin: NF-H Antibody (NF-01) [NB500-416] - NF200 in rat cerebellum. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, MaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, In vitro
Clone
NF-01
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NF-H Antibody (NF-01) Summary

Immunogen
A pellet of pig brain cold-stable proteins after depolymerization of microtubules.
Marker
Neuronal Marker
Specificity
The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NEFH
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • In vitro assay
Application Notes
Purity > 95% (by SDS-PAGE). Use in In vitro reported in scientific literature (PMID 25993115)
Theoretical MW
210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NF-H Lysate (NBP2-65371)
Reviewed Applications
Read 2 Reviews rated 5
using
NB500-416 in the following applications:

Publications
Read Publication using
NB500-416 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for NF-H Antibody (NF-01)

  • 200 kDa neurofilament protein
  • KIAA0845
  • NEFH
  • Neurofilament Heavy (200kDa)
  • neurofilament heavy polypeptide
  • Neurofilament triplet H protein
  • neurofilament, heavy polypeptide 200kDa
  • neurofilament, heavy polypeptide
  • NF200
  • NFH
  • NF-H

Background

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. When visualised using an electron microscope, neurofilaments appear as 10nm diameter fibres of indeterminate length that generally have fine wispy protrusions from their sides. They are particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed of a mixture of subunits, which usually includes the three neurofilament triplet proteins neurofilament light (NFL), neurofilament medium (NFM) and neurofilament heavy (NFH). Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. Some studies have shown that levels of neurofilament heavy and neurofilament light are elevated in patients with Alzheimer's disease, frontotemporal lobe dementia, and vascular dementia. This antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32 enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, this antibody labels a neuronal population with enhanced vulnerability to kainate toxicity, most of which are GABAergic and reveal kainate activated Ca2+ uptake.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NF-H Antibody (NB500-416)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: In vitro.


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In vitro
(1)
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Mouse
(1)
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Reviews for NF-H Antibody (NB500-416) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NB500-416:
Filter by Applications
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin NF-H NB500-416
Enlarge
reviewed by:
Anonymous
IHC-P Rat 07/07/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedrat brain (cerebellum)
SpeciesRat

Primary Anitbody

Dilution Ratio1:500

Details

Fixation DetailsFFPE HIER
 
reviewed by:
Anonymous
IF Mouse 04/20/2009
View

Summary

ApplicationImmunofluorescence
Sample Testednerve tissue from mouse spinal cord
SpeciesMouse
Commentsexcellent product

Comments

Commentsexcellent product

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NF-H Antibody (NB500-416) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-416AF405
Alexa Fluor 488 Labeled NB500-416AF488
Alexa Fluor 647 Labeled NB500-416AF647
Alexa Fluor 700 Labeled NB500-416AF700
DyLight 350 Labeled NB500-416UV
DyLight 405 Labeled NB500-416V
DyLight 488 Labeled NB500-416G
DyLight 680 Labeled NB500-416FR
DyLight 755 Labeled NB500-416IR

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
07/07/2014
Application: IHC-P
Species: Rat

 
Anonymous
04/20/2009
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NEFH
Entrez