NF-H Antibody (NF-01) [Alexa Fluor® 488]

Images

 
Immunofluorescence: NF-H Antibody (NF-01) [Alexa Fluor (R) 488] [NB500-416AF488] - IF staining of NF-H in frozen monkey foot pad skin shows sensory fibers. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, MaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
NF-01
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

NF-H Antibody (NF-01) [Alexa Fluor® 488] Summary

Immunogen
A pellet of pig brain cold-stable proteins after depolymerization of microtubules.
Marker
Neuronal Marker
Specificity
The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NEFH
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Reviewed Applications
Read 1 Review rated 5
using
NB500-416AF488 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for NF-H Antibody (NF-01) [Alexa Fluor® 488]

  • 200 kDa neurofilament protein
  • KIAA0845
  • NEFH
  • Neurofilament Heavy (200kDa)
  • neurofilament heavy polypeptide
  • Neurofilament triplet H protein
  • neurofilament, heavy polypeptide 200kDa
  • neurofilament, heavy polypeptide
  • NF200
  • NFH
  • NF-H

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NF-H Antibody (NB500-416AF488) (0)

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Review for NF-H Antibody (NB500-416AF488) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: IF.

Reviews using NB500-416AF488:
Filter by Applications
IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence NF-H NB500-416AF488
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reviewed by:
Anonymous
IF Other 02/24/2016
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Summary

ApplicationImmunofluorescence
Sample Testedfoot pad skin
SpeciesOther
LotSales order #217634

Blocking

Blocking Details"Superblock"

Primary Anitbody

Dilution RatioAntibody carrier 4% goat serum and.1% Triton-X in.1 M Tris buffer at pH 7.6; 24 h incubation at 4 C.

Details

Detection NotesLeica confocal flattened z-stack optical sections. 488 ex, emitted light collected at 498-550, original image 2048x2048 pixels
Fixation Details4% PFA fixation. 20% glycerol cyroprotectant, frozen and stored at -80 C.

Comments

CommentsImage processed in FIJI, and cropped to feature region of interest.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NF-H Antibody (NB500-416AF488) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NB500-416AF647
Alexa Fluor 700 Labeled NB500-416AF700

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Bioinformatics Tool for NF-H Antibody (NB500-416AF488)

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Blogs on NF-H

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
02/24/2016
Application: IF
Species: Other

Bioinformatics

Gene Symbol NEFH
Entrez