Neurturin Antibody - BSA Free

Western Blot: Neurturin Antibody [NBP1-77047] - HeLa cell lyaste containing 10 or 2.5 ng of full length recombinant NTN with neurturin antibody at 1:500.

Immunocytochemistry/ Immunofluorescence: Neurturin Antibody - BSA Free [NBP1-77047] - Immunofluorescence of Neurturin in mouse brain tissue with Neurturin Antibody at 20 ug/mL.Green: Neuriturin Antibody Red: Phylloidin staining Blue: DAPI staining

Immunohistochemistry: Neurturin Antibody - BSA Free [NBP1-77047] - Immunohistochemistry of Neurturin in mouse brain tissue with Neurturin Antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: Neurturin Antibody - BSA Free [NBP1-77047] - Immunofluorescence of Neurturin in human brain tissue with Neurturin antibody at 5 ug/ml.Green: Neuriturin Antibody Red: Phylloidin staining Blue: DAPI staining

Immunohistochemistry: Neurturin Antibody - BSA Free [NBP1-77047] - Immunohistochemistry of neurturin in human brain tissue with neurturin antibody at 5 ug/mL.

Immunohistochemistry: Neurturin Antibody - BSA Free [NBP1-77047] - Immunohistochemistry of Neurturin in human brain tissue with Neurturin antibody at 5 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Neurturin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Neurturin Antibody - BSA Free (NBP1-77047) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Neurturin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Neurturin. The immunogen is located within the last 50 amino acids of Neurturin. Amino Acid Squence: DAHSRYHTVHELSARE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NRTN
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:100-1:2000
• Application Notes: An approximate 14 kDa band of the full length recombinant Neurturin was detected in Western Blot.
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Neurturin Peptide (NBP1-77047PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Neurturin Antibody - BSA Free

• Neurturin
• NRTN
• NTN

Background

Glial cell line-derived neurotrophic factor (GDNF) plays key roles in the control of vertebrate neuron survival and differentiation. A novel neurotrophic factor was recently cloned from human and mouse and designated neurturin. Physiological responses to neurturin (NTN) require the presence of receptor tyrosine kinase RET and a novel glycosylphosphatidylinositol linked receptor NTNRalpha. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently and termed GDNFRbeta, Ret ligand 2 (RETL2) or TGF-beta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRalpha-2 recently. NTN binds to and forms a complex with GFRalpha-2 and the Ret PTK and activates the RET receptor tyrosine kinase pathway. Both NTN and GDNF can activate the MAP kinase and phosphatidylinositol 3-kinase pathways and play a critical role in the development of many neuronal populations. Neurturin and GDNF define a new family of neurotrophic factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurturin Antibody (NBP1-77047)(1)

Publication using NBP1-77047

• Jones MR, Jones J, Pandu P et Al. Neurturin GF Enhances the Acute Cytokine Response of Inflamed Skin J Invest Dermatol 2024-08-07 [PMID: 39122143]

Bioinformatics

• Gene Symbol: NRTN
• Uniprot:
  • NP_004549(Human)