GFR alpha-3/GDNF R alpha-3 Antibody [Unconjugated]

Images

 
There are currently no images for GFR alpha-3/GDNF R alpha-3 Antibody (AF2645).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

GFR alpha-3/GDNF R alpha-3 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse GFR alpha -3/GDNF R alpha -3
Glu34-Arg379
Accession # AAB70931
Specificity
Detects mouse GFR alpha -3/GDNF R alpha -3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human GFR alpha -3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GFRA3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF2645 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-3/GDNF R alpha-3 Antibody [Unconjugated]

  • GDNF family receptor alpha 3
  • GDNF family receptor alpha-3
  • GDNF R alpha-3
  • GDNF receptor alpha-3
  • GDNFR-alpha-3
  • GFR alpha3
  • GFR alpha-3
  • GFRA3
  • GFRa-3
  • GFR-alpha-3
  • glial cell line-derived neurotrophic factor receptor alpha-3
  • GPI-linked receptor

Background

Mouse glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 3 (GFR alpha -3) is a member of the GDNF family of receptors. It is one of four known members, all of which contain three conserved cysteine repeats (1, 3, 4). It is synthesized as a 397 aa precursor, with a hydrophobic signal sequence of 28 aa, a 343 aa mature segment, and a propeptide segment of 26 aa that is removed in the mature protein. The mature globular membrane glycoprotein has an approximate molecular weight of 50 kDa (5), contains three potential N-linked glycosylation sites, and a hydrophobic stretch of residues at its COOH terminus that comprises a GPI linkage sequence (2, 3, 5). Mouse GFR alpha -3 shares 81% aa sequence identity with human GFR alpha -3. High-level expression of GFR alpha -3 is observed only during early stages of neurogenesis in the central nervous system and in developing and adult peripheral nerves, organs, and ganglia (2, 3, 5, 7). The expression and proportions of GFR alpha -3 are closely linked to those of the Ret receptor tyrosine kinase, particularly in the trigeminal ganglion, pituitary gland, thymus, lung, and duodenum (3). GFR alpha -3 associates with Ret to form the receptor complex for the GDNF family ligand artemin, which, in turn, activates the complex (1, 6, 7). The activated complex’s signal is required for the development and maintenance of superior cervical ganglion (SCG), specifically the rostral migration of SCG precursors between embryonic days 11.5 and 14.5, and the survival of SCG neurons after birth (8).

  1. Xinquan, W. et al. (2006) Structure 14:1083.
  2. Worby, C. et al. (1998) J. Biol. Chem. 273:3502.
  3. Naveilhan, P. et al. (1998) Proc. Natl. Acad. Sci. USA 95:1295.
  4. Nomoto, S. et al. (1998) Biochem. Biophys. Res. Commun. 244:849.
  5. Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  6. Saarma, M. (2000) Eur. J. Biochem. 267:6968.
  7. Baloh, R. et al. (1998) Neuron 21:1291.
  8. Nishino, J et al. (1999) Neuron 23:725.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF482
Species: Mu
Applications: WB, IHC
477-MN/CF
Species: Mu
AF429
Species: Hu, Mu
Applications: WB, IHC, Block, ICC
MAB2388
Species: Hu
Applications: WB, IHC
NBP2-57362
Species: Hu
Applications: WB, ICC/IF
NBP2-24653
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-02541
Species: Hu, Mu, Rt, Pm, Pm
Applications: WB, ICC/IF, IHC, IHC-P
AF175
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
NB100-1617
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
DUP00
Species: Hu
AF2645
Species: Mu
Applications: WB, IHC

Publications for GFR alpha-3/GDNF R alpha-3 Antibody (AF2645)(5)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


Filter By Application
ICC
(2)
IHC
(2)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(4)
Rat
(1)
All Species

Reviews for GFR alpha-3/GDNF R alpha-3 Antibody (AF2645) (0)

There are no reviews for GFR alpha-3/GDNF R alpha-3 Antibody (AF2645). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFR alpha-3/GDNF R alpha-3 Antibody (AF2645) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2645

Additional GFR alpha-3/GDNF R alpha-3 Products

Bioinformatics Tool for GFR alpha-3/GDNF R alpha-3 Antibody (AF2645)

Discover related pathways, diseases and genes to GFR alpha-3/GDNF R alpha-3 Antibody (AF2645). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GFR alpha-3/GDNF R alpha-3 Antibody (AF2645)

Discover more about diseases related to GFR alpha-3/GDNF R alpha-3 Antibody (AF2645).
 

Pathways for GFR alpha-3/GDNF R alpha-3 Antibody (AF2645)

View related products by pathway.

PTMs for GFR alpha-3/GDNF R alpha-3 Antibody (AF2645)

Learn more about PTMs related to GFR alpha-3/GDNF R alpha-3 Antibody (AF2645).

Blogs on GFR alpha-3/GDNF R alpha-3

There are no specific blogs for GFR alpha-3/GDNF R alpha-3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GFR alpha-3/GDNF R alpha-3 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol GFRA3
Uniprot