GFR alpha-2/GDNF R alpha-2 Antibody [Unconjugated]

Images

 
GFR alpha ‑2/GDNF R alpha ‑2 was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse GFR alpha ‑2/GDNF R alpha ‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF429) at 5 ...read more
GFR alpha ‑2/GDNF R alpha ‑2 was detected in perfusion fixed frozen sections of mouse dorsal root ganglion using Goat Anti-Human/Mouse GFR alpha ‑2/GDNF R alpha ‑2 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

GFR alpha-2/GDNF R alpha-2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse GFR alpha ‑2/GDNF R alpha ‑2
Ser22-Ser441
Accession # Q3UUD8
Specificity
Detects human and mouse GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
5% cross‑reactivity with recombinant human GFR alpha -3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GFRA2
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF429 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-2/GDNF R alpha-2 Antibody [Unconjugated]

  • GDNF family receptor alpha 2
  • GDNF family receptor alpha-2
  • GDNF R alpha-2
  • GDNF receptor alpha-2
  • GDNF receptor beta
  • GDNFR-alpha-2
  • GDNFR-beta
  • GDNFRBRET ligand 2
  • GFR alpha2
  • GFR alpha-2
  • GFRA2
  • GFRa-2
  • GFR-alpha 2
  • GFR-alpha-2
  • glial cell line derived neurotrophic factor receptor, beta
  • Neurturin receptor alpha
  • NRTNR-alpha
  • NTNRA
  • NTNR-alpha
  • PI-linked cell-surface accessory protein
  • RETL2TGF-beta-related neurotrophic factor receptor 2
  • TGF-beta related neurotrophic factor receptor 2
  • TRN receptor, GPI-anchored
  • TRNR2NRTNR-ALPHA

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits, GDNF receptor alpha -1 (GFR alpha -1, also known as Trn R1) or GFR alpha -2 (also known as Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.

Mouse GFR alpha -2 cDNA encodes a 463 amino acid (aa) residue protein with a putative N-terminal 21 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a 3 aa residue (SGS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  4. Baloh, R.H. et al. (1998) Neuron 21:1291.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF482
Species: Mu
Applications: WB, IHC
AF1085
Species: Mu
Applications: WB, IHC, Neut
AF2645
Species: Mu
Applications: WB, IHC
MAB2388
Species: Hu
Applications: WB, IHC
NBP2-57362
Species: Hu
Applications: WB, ICC/IF
AF175
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
H00001958-M03
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, IHC-FrFl, IHC-WhMt, KO
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for GFR alpha-2/GDNF R alpha-2 Antibody (AF429)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, IHC-P, Neutralization.


Filter By Application
ICC
(4)
IHC
(5)
IHC-P
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(7)
Rat
(3)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF429 Applications Species
JNA Ferreira, C Zheng, IMA Lombaert, CM Goldsmith, AP Cotrim, JM Symonds, VN Patel, MP Hoffman Neurturin Gene Therapy Protects Parasympathetic Function to Prevent Irradiation-Induced Murine Salivary Gland Hypofunction Mol Ther Methods Clin Dev, 2018;9(0):172-180. 2018 [PMID: 29560384] (IHC, Mouse) IHC Mouse
S Kim, GJ Moon, YS Oh, J Park, WH Shin, JY Jeong, KS Choi, BK Jin, N Kholodilov, RE Burke, HJ Kim, CM Ha, SG Lee, SR Kim Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo Exp. Mol. Med., 2018;50(2):e440. 2018 [PMID: 29422542] (Neutralization, Mouse) Neutralization Mouse
E Emmerson, AJ May, L Berthoin, N Cruz-Pache, S Nathan, AJ Mattingly, JL Chang, WR Ryan, AD Tward, SM Knox Salivary glands regenerate after radiation injury through SOX2-mediated secretory cell replacement EMBO Mol Med, 2018;0(0):. 2018 [PMID: 29335337] (IHC-P, Human) IHC-P Human
JL Trevaskis, CB Sacramento, H Jouihan, S Ali, J Le Lay, S Oldham, N Bhagroo, BB Boland, J Cann, Y Chang, T O'Day, V Howard, C Reers, MS Winzell, DM Smith, M Feigh, P Barkholt, K Schreiter, M Austen, U Andag, S Thompson, L Jermutus, MP Coghlan, J Grimsby, C Dohrmann, CJ Rhodes, CM Rondinone, A Sharma Neurturin and a Glp-1 Analogue Act Synergistically to Alleviate Diabetes in Zucker Diabetic Fatty Rats Diabetes, 2017;0(0):. 2017 [PMID: 28408435] (IHC, Rat) IHC Rat
Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci, 2015;18(1):145-53. 2015 [PMID: 25420068] (IHC, Mouse) IHC Mouse
Knox, Sarah M, Lombaert, Isabelle, Haddox, Candace, Abrams, Shaun R, Cotrim, Ana, Wilson, Adrian J, Hoffman, Matthew Parasympathetic stimulation improves epithelial organ regeneration. Nat Commun, 2013;4(0):1494. 2013 [PMID: 23422662] (IHC, Mouse) IHC Mouse
Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo. J. Neurosci., 2006;26(33):8588-99. 2006 [PMID: 16914685] (ICC, Mouse) ICC Mouse
Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats. Stroke, 2006;37(9):2361-7. 2006 [PMID: 16873711] (ICC, Rat) ICC Rat
Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM Expression and function of GDNF family ligands and receptors in the carotid body. Exp. Neurol., 2005;191(0):S68-79. 2005 [PMID: 15629763] (ICC, Rat) ICC Rat
Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM, Choi DW, Snider BJ Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells. Exp. Neurol., 2005;191(1):65-76. 2005 [PMID: 15589513] (ICC, Mouse) ICC Mouse
Show All 11 Publications.

Reviews for GFR alpha-2/GDNF R alpha-2 Antibody (AF429) (0)

There are no reviews for GFR alpha-2/GDNF R alpha-2 Antibody (AF429). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFR alpha-2/GDNF R alpha-2 Antibody (AF429) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF429

Additional GFR alpha-2/GDNF R alpha-2 Products

Bioinformatics Tool for GFR alpha-2/GDNF R alpha-2 Antibody (AF429)

Discover related pathways, diseases and genes to GFR alpha-2/GDNF R alpha-2 Antibody (AF429). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GFR alpha-2/GDNF R alpha-2 Antibody (AF429)

Discover more about diseases related to GFR alpha-2/GDNF R alpha-2 Antibody (AF429).
 

Pathways for GFR alpha-2/GDNF R alpha-2 Antibody (AF429)

View related products by pathway.

PTMs for GFR alpha-2/GDNF R alpha-2 Antibody (AF429)

Learn more about PTMs related to GFR alpha-2/GDNF R alpha-2 Antibody (AF429).

Blogs on GFR alpha-2/GDNF R alpha-2

There are no specific blogs for GFR alpha-2/GDNF R alpha-2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GFR alpha-2/GDNF R alpha-2 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol GFRA2
Uniprot