GFR alpha-2/GDNF R alpha-2 Antibody [Unconjugated]


GFR alpha ‑2/GDNF R alpha ‑2 was detected in perfusion fixed frozen sections of mouse dorsal root ganglion using Goat Anti-Human/Mouse GFR alpha ‑2/GDNF R alpha ‑2 Antigen Affinity-purified Polyclonal Antibody more
GFR alpha ‑2/GDNF R alpha ‑2 was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse GFR alpha ‑2/GDNF R alpha ‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF429) at 5 more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF

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GFR alpha-2/GDNF R alpha-2 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse GFR alpha ‑2/GDNF R alpha ‑2
Accession # Q3UUD8
Detects human and mouse GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
5% cross‑reactivity with recombinant human GFR alpha -3 is observed.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Blockade of Receptor-ligand Interaction
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF429 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-2/GDNF R alpha-2 Antibody [Unconjugated]

  • GDNF family receptor alpha 2
  • GDNF family receptor alpha-2
  • GDNF R alpha-2
  • GDNF receptor alpha-2
  • GDNF receptor beta
  • GDNFR-alpha-2
  • GDNFR-beta
  • GDNFRBRET ligand 2
  • GFR alpha2
  • GFR alpha-2
  • GFRA2
  • GFRa-2
  • GFR-alpha 2
  • GFR-alpha-2
  • glial cell line derived neurotrophic factor receptor, beta
  • Neurturin receptor alpha
  • NRTNR-alpha
  • NTNR-alpha
  • PI-linked cell-surface accessory protein
  • RETL2TGF-beta-related neurotrophic factor receptor 2
  • TGF-beta related neurotrophic factor receptor 2
  • TRN receptor, GPI-anchored


Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits, GDNF receptor alpha -1 (GFR alpha -1, also known as Trn R1) or GFR alpha -2 (also known as Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.

Mouse GFR alpha -2 cDNA encodes a 463 amino acid (aa) residue protein with a putative N-terminal 21 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a 3 aa residue (SGS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  4. Baloh, R.H. et al. (1998) Neuron 21:1291.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rb, Rt
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Species: Ch, Dr, Hu, I, Ma, Mu, Rt
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Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
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Publications for GFR alpha-2/GDNF R alpha-2 Antibody (AF429)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, IHC-P, Neutralization.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF429 Applications Species
M Defaye, NS Abdullah, M Iftinca, A Hassan, F Agosti, Z Zhang, M Cumenal, GW Zamponi, C Altier Gut-innervating TRPV1+ neurons drive chronic visceral pain via microglial P2Y12 receptor Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):. 2021 [PMID: 34954381] (IHC, Mouse) IHC Mouse
JNA Ferreira, C Zheng, IMA Lombaert, CM Goldsmith, AP Cotrim, JM Symonds, VN Patel, MP Hoffman Neurturin Gene Therapy Protects Parasympathetic Function to Prevent Irradiation-Induced Murine Salivary Gland Hypofunction Mol Ther Methods Clin Dev, 2018;9(0):172-180. 2018 [PMID: 29560384] (IHC, Mouse) IHC Mouse
S Kim, GJ Moon, YS Oh, J Park, WH Shin, JY Jeong, KS Choi, BK Jin, N Kholodilov, RE Burke, HJ Kim, CM Ha, SG Lee, SR Kim Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo Exp. Mol. Med., 2018;50(2):e440. 2018 [PMID: 29422542] (Neutralization, Mouse) Neutralization Mouse
E Emmerson, AJ May, L Berthoin, N Cruz-Pache, S Nathan, AJ Mattingly, JL Chang, WR Ryan, AD Tward, SM Knox Salivary glands regenerate after radiation injury through SOX2-mediated secretory cell replacement EMBO Mol Med, 2018;0(0):. 2018 [PMID: 29335337] (IHC-P, Human) IHC-P Human
JL Trevaskis, CB Sacramento, H Jouihan, S Ali, J Le Lay, S Oldham, N Bhagroo, BB Boland, J Cann, Y Chang, T O'Day, V Howard, C Reers, MS Winzell, DM Smith, M Feigh, P Barkholt, K Schreiter, M Austen, U Andag, S Thompson, L Jermutus, MP Coghlan, J Grimsby, C Dohrmann, CJ Rhodes, CM Rondinone, A Sharma Neurturin and a Glp-1 Analogue Act Synergistically to Alleviate Diabetes in Zucker Diabetic Fatty Rats Diabetes, 2017;0(0):. 2017 [PMID: 28408435] (IHC, Rat) IHC Rat
Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci, 2015;18(1):145-53. 2015 [PMID: 25420068] (IHC, Mouse) IHC Mouse
Knox , Sarah M, Lombaert , Isabelle, Haddox , Candace, Abrams , Shaun R, Cotrim , Ana, Wilson , Adrian J, Hoffman , Matthew Parasympathetic stimulation improves epithelial organ regeneration. Nat Commun, 2013;4(0):1494. 2013 [PMID: 23422662] (IHC, Mouse) IHC Mouse
Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo. J. Neurosci., 2006;26(33):8588-99. 2006 [PMID: 16914685] (ICC, Mouse) ICC Mouse
Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats. Stroke, 2006;37(9):2361-7. 2006 [PMID: 16873711] (ICC, Rat) ICC Rat
Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM Expression and function of GDNF family ligands and receptors in the carotid body. Exp. Neurol., 2005;191(0):S68-79. 2005 [PMID: 15629763] (ICC, Rat) ICC Rat
Show All 12 Publications.

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Gene Symbol GFRA2