GFR alpha-2/GDNF R alpha-2 Antibody [Unconjugated]

GFR alpha ‑2/GDNF R alpha ‑2 was detected in perfusion fixed frozen sections of mouse dorsal root ganglion using Goat Anti-Human/Mouse GFR alpha ‑2/GDNF R alpha ‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF429) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of sensory neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

GFR alpha ‑2/GDNF R alpha ‑2 was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse GFR alpha ‑2/GDNF R alpha ‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF429) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu, Mu
• Applications: WB, IHC, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GFR alpha-2/GDNF R alpha-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse GFR alpha ‑2/GDNF R alpha ‑2
  Ser22-Ser441
  Accession # Q3UUD8
• Specificity: Detects human and mouse GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
  5% cross‑reactivity with recombinant human GFR alpha -3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GFRA2
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-2/GDNF R alpha-2 Antibody [Unconjugated]

• GDNF family receptor alpha 2
• GDNF family receptor alpha-2
• GDNF R alpha-2
• GDNF receptor alpha-2
• GDNF receptor beta
• GDNFR-alpha-2
• GDNFR-beta
• GDNFRBRET ligand 2
• GFR alpha2
• GFR alpha-2
• GFRA2
• GFRa-2
• GFR-alpha 2
• GFR-alpha-2
• glial cell line derived neurotrophic factor receptor, beta
• Neurturin receptor alpha
• NRTNR-alpha
• NTNRA
• NTNR-alpha
• PI-linked cell-surface accessory protein
• RETL2TGF-beta-related neurotrophic factor receptor 2
• TGF-beta related neurotrophic factor receptor 2
• TRN receptor, GPI-anchored
• TRNR2NRTNR-ALPHA

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits, GDNF receptor alpha -1 (GFR alpha -1, also known as Trn R1) or GFR alpha -2 (also known as Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.

Mouse GFR alpha -2 cDNA encodes a 463 amino acid (aa) residue protein with a putative N-terminal 21 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a 3 aa residue (SGS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
4. Baloh, R.H. et al. (1998) Neuron 21:1291.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFR alpha-2/GDNF R alpha-2 Antibody (AF429)(12)

Publications using AF429

• M Defaye, NS Abdullah, M Iftinca, A Hassan, F Agosti, Z Zhang, M Cumenal, GW Zamponi, C Altier Gut-innervating TRPV1+ neurons drive chronic visceral pain via microglial P2Y12 receptor Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):. 2021 [PMID: 34954381] (IHC, Mouse)
• JNA Ferreira, C Zheng, IMA Lombaert, CM Goldsmith, AP Cotrim, JM Symonds, VN Patel, MP Hoffman Neurturin Gene Therapy Protects Parasympathetic Function to Prevent Irradiation-Induced Murine Salivary Gland Hypofunction Mol Ther Methods Clin Dev, 2018;9(0):172-180. 2018 [PMID: 29560384] (IHC, Mouse)
• S Kim, GJ Moon, YS Oh, J Park, WH Shin, JY Jeong, KS Choi, BK Jin, N Kholodilov, RE Burke, HJ Kim, CM Ha, SG Lee, SR Kim Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo Exp. Mol. Med., 2018;50(2):e440. 2018 [PMID: 29422542] (Neutralization, Mouse)
• E Emmerson, AJ May, L Berthoin, N Cruz-Pache, S Nathan, AJ Mattingly, JL Chang, WR Ryan, AD Tward, SM Knox Salivary glands regenerate after radiation injury through SOX2-mediated secretory cell replacement EMBO Mol Med, 2018;0(0):. 2018 [PMID: 29335337] (IHC-P, Human)
• JL Trevaskis, CB Sacramento, H Jouihan, S Ali, J Le Lay, S Oldham, N Bhagroo, BB Boland, J Cann, Y Chang, T O'Day, V Howard, C Reers, MS Winzell, DM Smith, M Feigh, P Barkholt, K Schreiter, M Austen, U Andag, S Thompson, L Jermutus, MP Coghlan, J Grimsby, C Dohrmann, CJ Rhodes, CM Rondinone, A Sharma Neurturin and a Glp-1 Analogue Act Synergistically to Alleviate Diabetes in Zucker Diabetic Fatty Rats Diabetes, 2017;0(0):. 2017 [PMID: 28408435] (IHC, Rat)
• Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci, 2015;18(1):145-53. 2015 [PMID: 25420068] (IHC, Mouse)
• Knox , Sarah M, Lombaert , Isabelle, Haddox , Candace, Abrams , Shaun R, Cotrim , Ana, Wilson , Adrian J, Hoffman , Matthew Parasympathetic stimulation improves epithelial organ regeneration. Nat Commun, 2013;4(0):1494. 2013 [PMID: 23422662] (IHC, Mouse)
• Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo. J. Neurosci., 2006;26(33):8588-99. 2006 [PMID: 16914685] (ICC, Mouse)
• Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats. Stroke, 2006;37(9):2361-7. 2006 [PMID: 16873711] (ICC, Rat)
• Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM Expression and function of GDNF family ligands and receptors in the carotid body. Exp. Neurol., 2005;191(0):S68-79. 2005 [PMID: 15629763] (ICC, Rat)
• Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM, Choi DW, Snider BJ Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells. Exp. Neurol., 2005;191(1):65-76. 2005 [PMID: 15589513] (ICC, Mouse)
• Hiltunen JO, Laurikainen A, Airaksinen MS, Saarma M GDNF family receptors in the embryonic and postnatal rat heart and reduced cholinergic innervation in mice hearts lacking ret or GFRalpha2. Dev. Dyn., 2000;219(1):28-39. 2000 [PMID: 10974669] (IHC, Mouse)

Bioinformatics

• Gene Symbol: GFRA2
• Uniprot:
  • Q3UUD8()