GFR alpha ‑2/GDNF R alpha ‑2 was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse GFR alpha ‑2/GDNF R alpha ‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF429) at 5 ...read more
GFR alpha ‑2/GDNF R alpha ‑2 was detected in perfusion fixed frozen sections of mouse dorsal root ganglion using Goat Anti-Human/Mouse GFR alpha ‑2/GDNF R alpha ‑2 Antigen Affinity-purified Polyclonal Antibody ...read more
GFR alpha-2/GDNF R alpha-2 Antibody [Unconjugated] Summary
Mouse myeloma cell line NS0-derived recombinant mouse GFR alpha ‑2/GDNF R alpha ‑2 Ser22-Ser441 Accession # Q3UUD8
Detects human and mouse GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human GFR alpha -3 is observed.
<0.10 EU per 1 μg of the antibody by the LAL method.
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Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of the TGF-beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits, GDNF receptor alpha -1 (GFR alpha -1, also known as Trn R1) or GFR alpha -2 (also known as Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.
Mouse GFR alpha -2 cDNA encodes a 463 amino acid (aa) residue protein with a putative N-terminal 21 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a 3 aa residue (SGS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Baloh, R.H. et al. (1998) Neuron 21:1291.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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