GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated] Summary
Mouse myeloma cell line NS0-derived recombinant rat GFR alpha ‑1/GDNF R alpha ‑1
Accession # Q62997
Detects rat GFR alpha ‑1/GDNF R alpha ‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human GFR alpha ‑1 is observed and less than 1% cross-reactivity with recombinant mouse GFR alpha ‑2 is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 0.2 ug/mL
- Immunohistochemistry 5-15 ug/mL
- Blockade of Receptor-ligand Interaction
Packaging, Storage & Formulations
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]
- GDNF family receptor alpha 1
- GDNF family receptor alpha-1
- GDNF R alpha-1
- GDNF receptor alpha-1
- GFR alpha1
- GFR alpha-1
- Glial cell line-derived neurotrophic factor receptor alpha
- GPI-linked anchor protein
- PI-linked cell-surface accessory protein
- RET ligand 1
- TGF-beta related neurotrophic factor receptor 1
- TGF-beta-related neurotrophic factor receptor 1
Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and
‑2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an
N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
- Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
- Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
- Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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