GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GFRa-1/GDNF Ra-1 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

GFRa-1/GDNF Ra-1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Ectopic RAR gamma expression by GFR alpha 1+ spermatogonia. (A) The CAG-CAT-3xFLAG-Rarg transgene. When CAT between the loxP sites is deleted by TM-activated Cre, FLAG-tagged RAR gamma is constitutively expressed under the control of the CAG promoter. (B) Experimental design of the fate analysis of GFR alpha 1+ cells with enforced FLAG-RAR gamma expression upon VA readministration in VAD mice, as shown in C-F. Gfra1-CreERT2; CAG-CAT-3xFLAG-Rarg transgenic mice were maintained in VAD and VA was administered 2 days after TM injection, as indicated. Testes were then processed for IF. (C,D) IF images of whole-mount seminiferous tubules of the mice described above, 2 days after VA injection, stained for FLAG-RAR gamma (green) and KIT (magenta) (C), and cell number relative to the number of initial induced cells (D). Data for GFP-labeled NGN3+ and GFR alpha 1+ cells are reproduced from Fig. 2C and Fig. 3C, respectively, for comparison. The mean±s.e.m. value of three testes is shown. *P<0.003 (t-test), compared with the values of FLAG-RAR gamma + GFR alpha 1+ cells at day 2. (E,F) Representative confocal images of the same field of whole-mounts of seminiferous tubules of mice treated as described above, at 2 days after VA injection; staining was performed for GFR alpha 1, KIT and FLAG (E). Open arrowheads, white arrowheads and small arrows indicate FLAG+ cells that are GFR alpha 1+/KIT+, GFR alpha 1+/KIT− and GFR alpha 1−/KIT+, respectively. (F) Quantitation of GFP+ and FLAG-RAR gamma + cells showing different patterns of GFR alpha 1 and KIT expression in Gfra1-CreERT2; CAG-CAT-EGFP and Gfra1-CreERT2; CAG-CAT-3xFLAG-Rarg mice, respectively. Cell numbers are shown above each bar. (G) Experimental design of the fate analysis of GFR alpha 1+ cells with enforced FLAG-RAR gamma expression under normal conditions, as shown in H-J. Gfra1-CreERT2; CAG-CAT-3xFLAG-Rarg transgenic mice were pulsed with TM at 13-17 weeks of age, and after 2 and 10 days their testes were processed for IF. (H) IF images of whole-mount seminiferous tubules 2 and 10 days after TM injection, stained for FLAG-RAR gamma and GFR alpha 1. (I,J) Numbers of GFR alpha 1+ Aundiff (magenta), GFR alpha 1− Aundiff (green), KIT+ (blue) spermatogonia and total cells (black) in either GFP-labeled (I) or FLAG-RAR gamma -expressing (J) cells of Gfra1-CreERT2; CAG-CAT-EGFP and Gfra1-CreERT2; CAG-CAT-3xFLAG-Rarg mice, respectively, following the schedule shown in G. The mean±s.e.m. of four (I) and three (J) testes are shown. *P<0.05 (t-test), compared with the values on day 2. Scale bars: 50 μm. Image collected and cropped by CiteAb from the following open publication (//journals.biologists.com/dev/article/doi/10.1242/dev.118695/258623/Hierarchical-differentiation-competence-in), licensed under a CC-BY license. Not internally tested by R&D Systems.

Fate of pulse-labeled GFR alpha 1+ spermatogonia during VAD/VA administration. (A) The experimental schedule for B and C. Two days after the TM pulse, Gfra1-CreERT2; CAG-CAT-EGFP transgenic mice maintained in VAD were injected with VA and then fed a normal diet. Testis samples were harvested at the indicated times. (B) Representative IF images of whole-mount seminiferous tubule 0, 2, 6 and 10 days after VA injection stained for GFP and GFR alpha 1. (C) The number of GFP-labeled GFR alpha 1+ Aundiff (magenta), GFR alpha 1− Aundiff (green), KIT+ (blue) spermatogonia and total labeled (black) cells. Shown is the mean±s.e.m. of five, five, seven, six, four and three testes for days 0, 2, 4, 6, 8 and 10, respectively. Data, except for those of GFR alpha 1+ cells, on days 2, 6, 8 and 10 were significantly different compared with the values on day 0 (P<0.03, t-test). (D) Schedule for E and F. After the TM pulse, Gfra1-CreERT2; CAG-CAT-EGFP transgenic mice were continually fed the VAD diet. (E) Representative IF images of whole-mount seminiferous tubule 2, 14 and 30 days after TM pulse stained for GFP and GFR alpha 1. (F) Number of GFP-labeled GFR alpha 1+ Aundiff (magenta) and GFR alpha 1− Aundiff (green) spermatogonia and total labeled cells (black), shown as the mean±s.e.m. of eight, three and five testes on days 0, 14 and 30, respectively. *P=0.032, **P<0.002 (t-test) compared with the values for day 2. Scale bars: 50 μm. Image collected and cropped by CiteAb from the following open publication (//journals.biologists.com/dev/article/doi/10.1242/dev.118695/258623/Hierarchical-differentiation-competence-in), licensed under a CC-BY license. Not internally tested by R&D Systems.

Characterization of GFR alpha 1 and co-receptor expression in neurons and synapses of the septum→Hb→IPN pathway.(A) dTomato epifluorescence (red) in sagittal sections of Gfra1dTOM mouse brain injected with tamoxifen at 3 months counterstained with DAPI (blue). Scale bar, 500 μm. (B) GFP (green) and ChAT (red) immunostaining in sagittal sections of a 3-month-old Gfra1GFP mouse brain counterstained with DAPI (blue). Scale bar, 400 μm. (C, D) GFP (green), ChAT (red), and SP (gray) immunostaining in coronal sections of the mHb (C) and IPN (D) of a 3-month-old Gfra1GFP mouse brain. Scale bars, 200 μm. (E) GFR alpha 1 immunostaining (red) in coronal sections of the mHb and IPN from a 3-month-old control (C57BL6/J) mouse. Scale bars, 200 μm. (F) GFP (green) and GFR alpha 1 (red) immunostaining in a coronal section containing the IPN of a 3-month-old Gad67Cre;R1CGfx/fx mouse. GFP signal represents the GABAergic cells that express Gfra1. Scale bars, 100 μm (merged) and 35 μm (inset). (G) GFP (green) and CR (red) immunostaining in coronal sections of the BAC and TS from a 3-month-old Gfra1GFP mouse brain counterstained with DAPI (blue). Scale bars, 100 μm (BAC) and 200 μm (TS). (H, I) Immunoblots of We, Ct, and Sn protein extracts from the mHb and IPN of 3-month-old C57BL6/J mice probed for GFR alpha 1 and NCAM (H) or RET and PSD95 (I). Tubulin was probed as loading control. (J, M) GFR alpha 1 (green) and PSD93 (red) immunostaining in the mHb (J) and IPN (L) of 3-month-old C57BL6/J mice. Scale bars, 10 μm (merged) and 5 μm (insets). Graphs show the quantification (± SEM) of GFR alpha 1, PSD93, and double-labeled puncta in mHb (K) and IPN (M). N = 5 mice (25–30 images per mouse per structure). The data underlying this figure can be found at //figshare.com/projects/Raw_Data_Fernandez-Suarez_et_al_2021/123406. 3V, 3th ventricle; ChAT, choline acetyltransferase; CR, calretinin; Ct, cytosolic protein fraction; dIPN, dorsal interpeduncular nucleus; dmHb, dorsal medial habenula; FR, fasciculus retroflexus; GFR alpha 1, glial cell–derived neurotrophic factor receptor alpha 1; Hipp, hippocampus; HTH, hypothalamus; IPL, lateral interpeduncular nucleus; IPN, interpeduncular nucleus; LHb, lateral habenula; LS, lateral septum; mHb, medial habenula; MS, medial septum; NCAM, neural cell adhesion molecule; PSD, postsynaptic density; Sn, synaptosome protein fraction; SP, substance P; Str, striatum; vIPN, ventral interpeduncular nucleus; vmHb, ventral medial habenula; We, whole protein extract. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34748545), licensed under a CC-BY license. Not internally tested by R&D Systems.

Spermatogenesis development of adult Mafb-cKO mice.Six-week-old mice were injected with tamoxifen and testes of cKO mice at 3- and 8-months of age were examined compared to those of age-matched controls (n = 4 for each group). (A) Testicular sections stained with Periodic acid-Schiff (PAS). (B) Immunostaining with various testicular cell types; undifferentiated spermatogonia (GFRA1 and PLZF), differentiated spermatogonia (KIT+ inside the tubule), spermatocytes (SCP3), spermatids (PNA-Lectin), Sertoli cells (SOX9 and Vimentin), and Leydig cells (KIT+ outside the tubule and STAR). The color of markers is indicated in the left boxes in panels. Colored arrows corresponding to the boxed markers are shown. (C) The expression level of the genes involved in testis function and germ cell development. The marker genes representing undifferentiated spermatogonia (Nanos3), differentiated spermatogonia (c-Kit), undifferentiated and differentiated spermatogonia (Sohlh1), differentiated spermatogonia and preleptotene spermatocytes (Stra8), spermatids (Prm2), Leydig cells (Hsd3b1), and Sertoli cells (Sox9) were analyzed by qRT-PCR. Each reaction was performed in duplicate for each gene. The data represent the means±SEM and are shown as relative mRNA expression after normalization to Hprt. *P<0.05. (D) The proportion of seminiferous stages: I-III, IV-VI, VII-VIII, and IX-XII from each genotype. *P<0.05. M; Month. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0190800), licensed under a CC-BY license. Not internally tested by R&D Systems.

Ectopic RAR gamma expression by GFR alpha 1+ spermatogonia. (A) The CAG-CAT-3xFLAG-Rarg transgene. When CAT between the loxP sites is deleted by TM-activated Cre, FLAG-tagged RAR gamma is constitutively expressed under the control of the CAG promoter. (B) Experimental design of the fate analysis of GFR alpha 1+ cells with enforced FLAG-RAR gamma expression upon VA readministration in VAD mice, as shown in C-F. Gfra1-CreERT2; CAG-CAT-3xFLAG-Rarg transgenic mice were maintained in VAD and VA was administered 2 days after TM injection, as indicated. Testes were then processed for IF. (C,D) IF images of whole-mount seminiferous tubules of the mice described above, 2 days after VA injection, stained for FLAG-RAR gamma (green) and KIT (magenta) (C), and cell number relative to the number of initial induced cells (D). Data for GFP-labeled NGN3+ and GFR alpha 1+ cells are reproduced from Fig. 2C and Fig. 3C, respectively, for comparison. The mean±s.e.m. value of three testes is shown. *P<0.003 (t-test), compared with the values of FLAG-RAR gamma + GFR alpha 1+ cells at day 2. (E,F) Representative confocal images of the same field of whole-mounts of seminiferous tubules of mice treated as described above, at 2 days after VA injection; staining was performed for GFR alpha 1, KIT and FLAG (E). Open arrowheads, white arrowheads and small arrows indicate FLAG+ cells that are GFR alpha 1+/KIT+, GFR alpha 1+/KIT− and GFR alpha 1−/KIT+, respectively. (F) Quantitation of GFP+ and FLAG-RAR gamma + cells showing different patterns of GFR alpha 1 and KIT expression in Gfra1-CreERT2; CAG-CAT-EGFP and Gfra1-CreERT2; CAG-CAT-3xFLAG-Rarg mice, respectively. Cell numbers are shown above each bar. (G) Experimental design of the fate analysis of GFR alpha 1+ cells with enforced FLAG-RAR gamma expression under normal conditions, as shown in H-J. Gfra1-CreERT2; CAG-CAT-3xFLAG-Rarg transgenic mice were pulsed with TM at 13-17 weeks of age, and after 2 and 10 days their testes were processed for IF. (H) IF images of whole-mount seminiferous tubules 2 and 10 days after TM injection, stained for FLAG-RAR gamma and GFR alpha 1. (I,J) Numbers of GFR alpha 1+ Aundiff (magenta), GFR alpha 1− Aundiff (green), KIT+ (blue) spermatogonia and total cells (black) in either GFP-labeled (I) or FLAG-RAR gamma -expressing (J) cells of Gfra1-CreERT2; CAG-CAT-EGFP and Gfra1-CreERT2; CAG-CAT-3xFLAG-Rarg mice, respectively, following the schedule shown in G. The mean±s.e.m. of four (I) and three (J) testes are shown. *P<0.05 (t-test), compared with the values on day 2. Scale bars: 50 μm. Image collected and cropped by CiteAb from the following open publication (//journals.biologists.com/dev/article/doi/10.1242/dev.118695/258623/Hierarchical-differentiation-competence-in), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of genes in the RA signaling pathway and the specificity of RAR gamma expression by NGN3+ spermatogonia. (A) Scatter plot comparing the levels of transcripts expressed by GFR alpha 1+ and NGN3+ cells according to microarray analysis. For each fraction, average values from three independent RNA samples sorted from different animals are shown. The middle line indicates a difference of 0, and the outer lines represent ratios {log2([NGN3+]/[GFR alpha 1+])} of 1.0 and −1.0. Red dots indicate genes expressed at significantly different levels (P<0.05, t-test with Benjamini–Hochberg correction). Members of the Rar and Rxr gene families are indicated. A list of the genes in this panel is shown in supplementary material Table S2. (B) qRT-PCR analysis of Rara and Rarg mRNA expression in GFR alpha 1+, NGN3+ and KIT+ spermatogonia. Relative copy numbers are shown in arbitrary units after normalization to the amount of Actb mRNA. The mean±s.e.m. values of three independent cell preparations from different animals are shown. *P=0.043, **P=0.011 and ***P=0.0065 (t-test). (C) Representative IF images of whole-mount seminiferous tubules from Ngn3-EGFP mice stained for EGFP, GFR alpha 1 and RAR gamma . (D,E) Frequency of single- and double-positive spermatogonia for the expression of RAR gamma and NGN3 (recognized as RAR gamma +/KIT− and NGN3-EGFP+/KIT−, respectively) (D) and of RAR gamma and GFR alpha 1 (E). Total counts are shown above each bar. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//journals.biologists.com/dev/article/doi/10.1242/dev.118695/258623/Hierarchical-differentiation-competence-in), licensed under a CC-BY license. Not internally tested by R&D Systems.

Fate of pulse-labeled GFR alpha 1+ spermatogonia during VAD/VA administration. (A) The experimental schedule for B and C. Two days after the TM pulse, Gfra1-CreERT2; CAG-CAT-EGFP transgenic mice maintained in VAD were injected with VA and then fed a normal diet. Testis samples were harvested at the indicated times. (B) Representative IF images of whole-mount seminiferous tubule 0, 2, 6 and 10 days after VA injection stained for GFP and GFR alpha 1. (C) The number of GFP-labeled GFR alpha 1+ Aundiff (magenta), GFR alpha 1− Aundiff (green), KIT+ (blue) spermatogonia and total labeled (black) cells. Shown is the mean±s.e.m. of five, five, seven, six, four and three testes for days 0, 2, 4, 6, 8 and 10, respectively. Data, except for those of GFR alpha 1+ cells, on days 2, 6, 8 and 10 were significantly different compared with the values on day 0 (P<0.03, t-test). (D) Schedule for E and F. After the TM pulse, Gfra1-CreERT2; CAG-CAT-EGFP transgenic mice were continually fed the VAD diet. (E) Representative IF images of whole-mount seminiferous tubule 2, 14 and 30 days after TM pulse stained for GFP and GFR alpha 1. (F) Number of GFP-labeled GFR alpha 1+ Aundiff (magenta) and GFR alpha 1− Aundiff (green) spermatogonia and total labeled cells (black), shown as the mean±s.e.m. of eight, three and five testes on days 0, 14 and 30, respectively. *P=0.032, **P<0.002 (t-test) compared with the values for day 2. Scale bars: 50 μm. Image collected and cropped by CiteAb from the following open publication (//journals.biologists.com/dev/article/doi/10.1242/dev.118695/258623/Hierarchical-differentiation-competence-in), licensed under a CC-BY license. Not internally tested by R&D Systems.

Retention of the spermatogonial phenotype following gene correction.Immunostaining was performed on gene-corrected GT59 (left) and GT65 cells (right): DAZL, a germ cell specific marker; GFRA1, POU5F1, ETV5, CDH1, and SOHLH1, markers of undifferentiated spermatogonia. Additionally, GT59 and GT65 cells were treated with the differentiation factor, retinoic acid (1 µM) or a vehicle control and then immunostained to examine levels of ZBTB16, a marker of undifferentiated spermatogonia. Bar represents 50 microns. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25409432), licensed under a CC-BY license. Not internally tested by R&D Systems.

Testis anatomy and spermatogonial populations and their kinetics in the VAD model. (A) Anatomy of seminiferous tubules and seminiferous epithelium. Aundiff spermatogonia, including GFR alpha 1+ (magenta) and NGN3+ (green) cells and KIT+ differentiating spermatogonia (blue), reside in the basal compartment (between the basement membrane and the tight junction of Sertoli cells). (B) Hierarchical and heterogeneous composition of spermatogonia. PL, preleptotene spermatocytes. (C) Representative whole-mount IF images of spermatogonia derived from an Ngn3-EGFP mouse triple stained for GFR alpha 1, GFP and KIT. (D) The experimental schedule for E and F. Wild-type VAD mice were injected with VA and fed a normal (VA-sufficient) diet thereafter, before analysis at the indicated times. (E) Representative images of in situ hybridization analysis of Gfra1, Ngn3 and Kit expression in testis sections. Arrowheads indicate spermatogonia expressing these genes. Note the persisting Kit expression in interstitial cells (asterisks). (F) Counts of Gfra1+, Ngn3+ and Kit+ spermatogonia. Raw counts are summarized in supplementary material Table S1. Scale bars: 100 μm. Image collected and cropped by CiteAb from the following open publication (//journals.biologists.com/dev/article/doi/10.1242/dev.118695/258623/Hierarchical-differentiation-competence-in), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Rt
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat GFR alpha ‑1/GDNF R alpha ‑1
  Asp25-Leu445
  Accession # Q62997
• Specificity: Detects rat GFR alpha -1/GDNF R alpha -1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human GFR alpha -1 is observed and less than 1% cross-reactivity with recombinant mouse GFR alpha -2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GFRA1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

• DKFZp313E1012
• DKFZp686J0156
• GDNF family receptor alpha 1
• GDNF family receptor alpha-1
• GDNF R alpha-1
• GDNF receptor alpha-1
• GDNFRAGFR-ALPHA-1
• GDNFR-alpha-1
• GDNFRMGC23045
• GFR alpha1
• GFR alpha-1
• GFRA1
• GFRa-1
• GFR-alpha-1
• Glial cell line-derived neurotrophic factor receptor alpha
• GPI-linked anchor protein
• PI-linked cell-surface accessory protein
• RET ligand 1
• RET1L
• RETL1FLJ10561
• TGF-beta related neurotrophic factor receptor 1
• TGF-beta-related neurotrophic factor receptor 1
• TRNR1FLJ31546

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFR alpha-1/GDNF R alpha-1 Antibody (AF560)(75)

Publications using AF560

• Yang, SH;Zeng, YZ;Jia, XZ;Gu, YW;Wood, C;Yang, RS;Yang, JS;Yang, WJ; Activated dormant stem cells recover spermatogenesis in chemoradiotherapy-induced infertility Cell reports 2024-08-02 [PMID: 39096488] (Immunohistochemistry, Mouse)
• Nitahara, K;Kawamura, A;Kitamura, Y;Kato, K;Namekawa, SH;Nishiyama, M; Chromatin remodeler CHD8 is required for spermatogonial proliferation and early meiotic progression Nucleic acids research 2024-01-16 [PMID: 38224953] (IHC, Transgenic Mouse)
• Anbarci, DN;McKey, J;Levic, DS;Bagnat, M;Capel, B; Rediscovering the Rete Ovarii: a secreting auxiliary structure to the ovary bioRxiv : the preprint server for biology 2023-11-11 [PMID: 37986754] (IHC, Mouse)
• Penny A. F. Whiley, Benedict Nathaniel, Peter G. Stanton, Robin M. Hobbs, Kate L. Loveland Spermatogonial fate in mice with increased activin A bioactivity and testicular somatic cell tumours Frontiers in Cell and Developmental Biology 7/26/2023 [PMID: 37564373]
• X Feng, T Matsumura, Y Yamashita, T Sato, K Hashimoto, H Odaka, Y Makino, Y Okada, H Nakamura, H Kimura, T Fujii, T Ogawa In vitro spermatogenesis in isolated seminiferous tubules of immature mice PLoS ONE, 2023;18(4):e0283773. 2023 [PMID: 37023052] (IHC, Mouse)
• X Feng, T Matsumura, Y Yamashita, T Sato, K Hashimoto, H Odaka, Y Makino, Y Okada, H Nakamura, H Kimura, T Fujii, T Ogawa In vitro spermatogenesis in isolated seminiferous tubules of immature mice PLoS ONE, 2023-04-06;18(4):e0283773. 2023-04-06 [PMID: 37023052] (IHC, Mouse)
• Yi Jacky Peng, Xinyu Thomas Tang, Hui Sophie Shu, Wenjie Dong, Hongfang Shao, Bo O. Zhou Sertoli cells are the unique source of stem cell factor for spermatogenesis Development 3/15/2023 [PMID: 36861441]
• A Uchida, K Imaimatsu, H Suzuki, X Han, H Ushioda, M Uemura, K Imura-Kish, R Hiramatsu, HM Takase, Y Hirate, A Ogura, M Kanai-Azum, A Kudo, Y Kanai SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse Nature Communications, 2022-12-21;13(1):7860. 2022-12-21 [PMID: 36543770] (IHC, Mouse)
• MN Khan, P Cherukuri, F Negro, A Rajput, P Fabrowski, V Bansal, C Lancelin, TI Lee, Y Bian, WP Mayer, T Akay, D Müller, S Bonn, D Farina, T Marquardt ERR2 and ERR3 promote the development of gamma motor neuron functional properties required for proprioceptive movement control PloS Biology, 2022-12-21;20(12):e3001923. 2022-12-21 [PMID: 36542664] (IHC, Human)
• Manuela Nieto-Rostro, Ryan Patel, Anthony H. Dickenson, Annette C. Dolphin Nerve injury increases native Ca V 2.2 trafficking in dorsal root ganglion mechanoreceptors Pain 6/1/2023 [PMID: 36524581]
• Quinton Blount, Ignacio Hernandez‐Morato, Yalda Moayedi, Michael J. Pitman Expression of Glial Cell‐Derived Neurotrophic Factor Receptors Within Nucleus Ambiguus During Rat Development The Laryngoscope 9/1/2023 [PMID: 36271908]
• Oleksandr Kirsanov, Taylor Johnson, Taylor Malachowski, Bryan A. Niedenberger, Emma A. Gilbert, Debajit Bhowmick et al. Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro Development 11/15/2022 [PMID: 36250451]
• Ram Prakash Yadav, Sini Leskinen, Lin Ma, Juho-Antti Mäkelä, Noora Kotaja Chromatin remodelers HELLS, WDHD1 and BAZ1A are dynamically expressed during mouse spermatogenesis Reproduction 1/1/2023 [PMID: 36194437]
• Hirano K, Nonami Y, Nakamura Y et al. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis Communications Biology 2022-05-26 [PMID: 35618762]
• Hirano K, Nonami Y, Nakamura Y et al. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis Communications Biology 5/26/2022 [PMID: 35618762]
• V Barroca, C Racine, L Pays, P Fouchet, M Coureuil, I Allemand The netrin-1 receptor UNC5C contributes to the homeostasis of undifferentiated spermatogonia in adult mice Stem Cell Research, 2022-02-25;60(0):102723. 2022-02-25 [PMID: 35247845] (IHC, Mouse)
• Qianxing Z, Lele Y, Ruona S et al. Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis. iScience. 2021-10-30 [PMID: 34825148]
• Qianxing Z, Lele Y, Ruona S et al. Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis. iScience. 10/30/2021 [PMID: 34825148]
• Vladimir Vladimirovich Muzyka, Tudor Constantin Badea Genetic interplay between transcription factor Pou4f1/Brn3a and neurotrophin receptor Ret in retinal ganglion cell type specification Neural Development 12/1/2021 [PMID: 34548095]
• Y Mori, S Takashima, M Kanatsu-Sh, Z Yi, T Shinohara Cdc42 is required for male germline niche development in mice Cell Reports, 2021-08-17;36(7):109550. 2021-08-17 [PMID: 34407418] (ICC, Mouse)
• T Yamamuro, S Nakamura, Y Yamano, T Endo, K Yanagawa, A Tokumura, T Matsumura, K Kobayashi, H Mori, Y Enokidani, G Yoshida, H Imoto, T Kawabata, M Hamasaki, A Kuma, S Kuribayash, K Takezawa, Y Okada, M Ozawa, S Fukuhara, T Shinohara, M Ikawa, T Yoshimori Rubicon prevents autophagic degradation of GATA4 to promote Sertoli cell function PloS Genetics, 2021-08-05;17(8):e1009688. 2021-08-05 [PMID: 34351902] (IHC, Mouse)
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• Emily E. Bosco, R. James Christie, Rosa Carrasco, Darrin Sabol, Jiping Zha, Karma DaCosta et al. Preclinical evaluation of a GFRA1 targeted antibody-drug conjugate in breast cancer Oncotarget 5/1/2018 [PMID: 29796165]
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• BA Niedenberg, CB Geyer Advanced immunostaining approaches to study early male germ cell development Stem Cell Res, 2018-02-09;0(0):. 2018-02-09 [PMID: 29475796] (IHC-Fr, Mouse)
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• JC Arnold, MA Cantu, EA Kasanga, VA Nejtek, EV Papa, N Bugnariu, MF Salvatore Aging-related limit of exercise efficacy on motor decline PLoS ONE, 2017-11-27;12(11):e0188538. 2017-11-27 [PMID: 29176896] (Western Blot, Rat)
• Dai MS, Hall SJ, Vantangoli Policelli MM et al. Spontaneous testicular atrophy occurs despite normal spermatogonial proliferation in a Tp53 knockout rat Andrology 8/22/2017 [PMID: 28834365]
• S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;114(12):E2486-E2493. 2017-03-07 [PMID: 28270618] (Neutralization, Mouse)
• Kellie S. Agrimson, Jennifer Onken, Debra Mitchell, Traci B. Topping, Hélio Chiarini-Garcia, Cathryn A. Hogarth et al. Characterizing the Spermatogonial Response to Retinoic Acid During the Onset of Spermatogenesis and Following Synchronization in the Neonatal Mouse Testis1 Biology of Reproduction 10/13/2016 [PMID: 27488029]
• Hong Soon Kang, Liang-Yu Chen, Kristin Lichti-Kaiser, Grace Liao, Kevin Gerrish, Carl D. Bortner et al. Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis Stem Cells 11/1/2016 [PMID: 27350140]
• Michael F. Salvatore, Jennifer Terrebonne, Victoria Fields, Danielle Nodurft, Cori Runfalo, Brian Latimer et al. Initiation of calorie restriction in middle-aged male rats attenuates aging-related motoric decline and bradykinesia without increased striatal dopamine Neurobiology of Aging 1/1/2016 [PMID: 26610387]
• Arnold JC, Salvatore MF. Exercise-mediated increase in nigral tyrosine hydroxylase is accompanied by increased nigral GFR-a1 and EAAC1 expression in aging rats. ACS Chem Neurosci. 11/24/2015 [PMID: 26599339]
• Jonathan T. Busada, Bryan A. Niedenberger, Ellen K. Velte, Brett D. Keiper, Christopher B. Geyer Mammalian target of rapamycin complex 1 (mTORC1) Is required for mouse spermatogonial differentiation in vivo Developmental Biology 11/1/2015 [PMID: 26254600]
• Shelley L. Forrest, Sophie C. Payne, Janet R. Keast, Peregrine B. Osborne Peripheral injury of pelvic visceral sensory nerves alters GFRα (GDNF family receptor alpha) localization in sensory and autonomic pathways of the sacral spinal cord Frontiers in Neuroanatomy 4/10/2015 [PMID: 25914629]
• Shelley L. Forrest, Sophie C. Payne, Janet R. Keast, Peregrine B. Osborne Peripheral injury of pelvic visceral sensory nerves alters GFRα (GDNF family receptor alpha) localization in sensory and autonomic pathways of the sacral spinal cord Frontiers in Neuroanatomy 2015-04-10 [PMID: 25914629] (Immunohistochemistry, Rat)
• Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors. Proc Natl Acad Sci U S A, 2015-03-09;112(15):E1946-55. 2015-03-09 [PMID: 25775569] (Flow Cytometry, IHC, Mouse, Rat)
• Bryan A. Niedenberger, Jonathan T. Busada, Christopher B. Geyer Marker expression reveals heterogeneity of spermatogonia in the neonatal mouse testis REPRODUCTION 4/1/2015 [PMID: 25737569]
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• Danielle A. Fanslow, Stacey E. Wirt, Jenny C. Barker, Jon P. Connelly, Matthew H. Porteus, Christina Tenenhaus Dann Genome Editing in Mouse Spermatogonial Stem/Progenitor Cells Using Engineered Nucleases PLoS ONE 11/19/2014 [PMID: 25409432]
• Mai Shinomura, Kasane Kishi, Ayako Tomita, Miyuri Kawasumi, Hiromi Kanezashi, Yoshiko Kuroda et al. A novel Amh-Treck transgenic mouse line allows toxin-dependent loss of supporting cells in gonads REPRODUCTION 12/1/2014 [PMID: 25212783]
• Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D PAX7 expression defines germline stem cells in the adult testis. J Clin Invest, 2014-08-18;124(9):3929-44. 2014-08-18 [PMID: 25133429] (IHC-P, Mouse)
• Chakraborty P, Buaas F, Sharma M, Snyder E, de Rooij D, Braun R LIN28A marks the spermatogonial progenitor population and regulates its cyclic expansion. Stem Cells, 2014-04-01;32(4):860-73. 2014-04-01 [PMID: 24715688] (IHC, Mouse)
• Shelley L. Forrest, Peregrine B. Osborne, Janet R. Keast Characterization of bladder sensory neurons in the context of myelination, receptors for pain modulators, and acute responses to bladder inflammation Frontiers in Neuroscience 11/7/2013 [PMID: 24223534]
• Yueh-Chiang Hu, Dirk G. de Rooij, David C. Page Tumor suppressor gene Rb is required for self-renewal of spermatogonial stem cells in mice Proceedings of the National Academy of Sciences 7/30/2013 [PMID: 23858447]
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Reviews for GFR alpha-1/GDNF R alpha-1 Antibody (AF560) (8)

Average Rating: 4.5

(Based on 8 reviews)

We have 8 reviews tested in 2 species: Mouse, Rat.

reviewed by: Verified Customer IF Mouse 01/08/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23012458
• Species: Mouse

reviewed by: Verified Customer IF Mouse 01/08/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22711565
• Species: Mouse

reviewed by: Verified Customer IF Rat 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Recombinant rat GFR alpha-1 expressed in 293 cells
• Species: Rat

reviewed by: Verified Customer IP Rat 12/18/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: Rat GFR alpha-1 expressed in 293 cells
• Species: Rat

reviewed by: Verified Customer IHC-Fr Mouse 12/18/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: PFA-fixed cryosections of mouse brain
• Species: Mouse

reviewed by: Verified Customer IF Mouse 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Hippocampal neurons
• Species: Mouse

reviewed by: Verified Customer WB Rat 12/18/2014

Summary

• Application: Western Blot
• Sample Tested: Recombinant rat GFR alpha-1 expressed in 293 cells
• Species: Rat

reviewed by: Verified Customer WB Mouse 12/18/2014

Summary

• Application: Western Blot
• Sample Tested: Hippocampal neurons
• Species: Mouse

Bioinformatics

• Gene Symbol: GFRA1
• Uniprot:
  • Q62997()