GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

Images

 
GFR alpha ‑1/GDNF R alpha ‑1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GFR alpha ‑1/GDNF R alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) at ...read more
Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat GFR alpha ‑1/GDNF R alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) followed by ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat GFR alpha ‑1/GDNF R alpha ‑1
Asp25-Leu445
Accession # Q62997
Specificity
Detects rat GFR alpha ‑1/GDNF R alpha ‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human GFR alpha ‑1 is observed and less than 1% cross-reactivity with recombinant mouse GFR alpha ‑2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GFRA1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 8 Reviews rated 4.5
using
AF560 in the following applications:

Publications
Read Publications using
AF560 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

  • DKFZp313E1012
  • DKFZp686J0156
  • GDNF family receptor alpha 1
  • GDNF family receptor alpha-1
  • GDNF R alpha-1
  • GDNF receptor alpha-1
  • GDNFRAGFR-ALPHA-1
  • GDNFR-alpha-1
  • GDNFRMGC23045
  • GFR alpha1
  • GFR alpha-1
  • GFRA1
  • GFRa-1
  • GFR-alpha-1
  • Glial cell line-derived neurotrophic factor receptor alpha
  • GPI-linked anchor protein
  • PI-linked cell-surface accessory protein
  • RET ligand 1
  • RET1L
  • RETL1FLJ10561
  • TGF-beta related neurotrophic factor receptor 1
  • TGF-beta-related neurotrophic factor receptor 1
  • TRNR1FLJ31546

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and
‑2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an
N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GFR alpha-1/GDNF R alpha-1 Antibody (AF560)(21)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Porcine.

We have publications tested in 7 applications: ICC, IHC, IHC-Fr, IHC-P, Immunochromatography, Neutralization, Western Blot.


Filter By Application
ICC
(4)
IHC
(6)
IHC-Fr
(2)
IHC-P
(3)
Immunochromatography
(1)
Neutralization
(2)
Western Blot
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(14)
Rat
(4)
Chicken
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using AF560 Applications Species
A Tsuchimoto, M Tone, N Ogonuki, M Hada, A Ogura, S Takashima Germ cell depletion in recipient testis has adverse effects on spermatogenesis in orthotopically transplanted testis pieces via retinoic acid insufficiency Sci Rep, 2020;10(1):10796. 2020 [PMID: 32612133] (IHC, Mouse) IHC Mouse
A Bonafina, MF Trinchero, AS Ríos, P Bekinschte, AF Schinder, G Paratcha, F Ledda GDNF and GFRalpha1 Are Required for Proper Integration of Adult-Born Hippocampal Neurons Cell Rep, 2019;29(13):4308-4319.e4. 2019 [PMID: 31875542] (IHC, Mouse) IHC Mouse
RL Tang, LQ Fan PLZFposc-KITpos-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation Asian J. Androl., 2019;21(3):309-318. 2019 [PMID: 30719983] (IHC, Mouse) IHC Mouse
BP Hermann, K Cheng, A Singh, L Roa-De La, KN Mutoji, IC Chen, H Gilderslee, JD Lehle, M Mayo, B Westernstr, NC Law, MJ Oatley, EK Velte, BA Niedenberg, D Fritze, S Silber, CB Geyer, JM Oatley, JR McCarrey The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids Cell Rep, 2018;25(6):1650-1667.e8. 2018 [PMID: 30404016] (IHC-Fr, Mouse) IHC-Fr Mouse
J Guo, EJ Grow, H Mlcochova, GJ Maher, C Lindskog, X Nie, Y Guo, Y Takei, J Yun, L Cai, R Kim, DT Carrell, A Goriely, JM Hotaling, BR Cairns The adult human testis transcriptional cell atlas Cell Res., 2018;0(0):. 2018 [PMID: 30315278] (IHC-P, Human) IHC-P Human
BA Niedenberg, CB Geyer Advanced immunostaining approaches to study early male germ cell development Stem Cell Res, 2018;0(0):. 2018 [PMID: 29475796] (IHC-Fr, Mouse) IHC-Fr Mouse
A Kurita-Suz, Y Kamo, C Uchida, K Tanemura, K Hara, T Uchida Prolyl isomerase Pin1 is required sperm production by promoting mitosis progression of spermatogonial stem cells Biochem. Biophys. Res. Commun., 2018;0(0):. 2018 [PMID: 29432730] (Immunochromatography, Mouse) Immunochromatography Mouse
JC Arnold, MA Cantu, EA Kasanga, VA Nejtek, EV Papa, N Bugnariu, MF Salvatore Aging-related limit of exercise efficacy on motor decline PLoS ONE, 2017;12(11):e0188538. 2017 [PMID: 29176896] (Western Blot, Rat) Western Blot Rat
S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493. 2017 [PMID: 28270618] (Neutralization, Mouse) Neutralization Mouse
Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors. Proc Natl Acad Sci U S A, 2015;112(15):E1946-55. 2015 [PMID: 25775569] (IHC, Mouse) IHC Mouse
Show All 21 Publications.

Reviews for GFR alpha-1/GDNF R alpha-1 Antibody (AF560) (8) 4.58

Average Rating: 4.5
(Based on 8 reviews)
We have 8 reviews tested in 2 species: Mouse, Rat.

Reviews using AF560:
Filter by Applications
IF
(4)
IP
(1)
IHC-Fr
(1)
WB
(2)
All Applications
Filter by Species
Mouse
(5)
Rat
(3)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23012458
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23012458

Details

Detection NotesSee PMID 23012458

Comments

CommentsPublished: See PMID 23012458Independent review from antibodyresource.com
  4
reviewed by:
Anonymous
IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22711565
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22711565

Details

Detection NotesSee PMID 22711565

Comments

CommentsPublished: See PMID 22711565
Immunofluorescence GFR alpha-1/GDNF R alpha-1 AF560
Enlarge
5
reviewed by:
Anonymous
IF Rat 12/18/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedRecombinant rat GFR alpha-1 expressed in 293 cells
SpeciesRat

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
  5
reviewed by:
Anonymous
IP Rat 12/18/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedRat GFR alpha-1 expressed in 293 cells
SpeciesRat

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
  3
reviewed by:
Anonymous
IHC-Fr Mouse 12/18/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedPFA-fixed cryosections of mouse brain
SpeciesMouse
  5
reviewed by:
Anonymous
IF Mouse 12/18/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHippocampal neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
  5
reviewed by:
Anonymous
WB Rat 12/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedRecombinant rat GFR alpha-1 expressed in 293 cells
SpeciesRat

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
  5
reviewed by:
Anonymous
WB Mouse 12/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedHippocampal neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/

Product General Protocols

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FAQs for GFR alpha-1/GDNF R alpha-1 Antibody (AF560) (0)

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Secondary Antibodies

 

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Biotin BAF560

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Recent Reviews

4.5
5
5
4
2
3
1
2
0
1
0

 
Anonymous
01/08/2015
Application: IF
Species: Mouse

 
Anonymous
01/08/2015
Application: IF
Species: Mouse

Anonymous
12/18/2014
Application: IF
Species: Rat

Bioinformatics

Gene Symbol GFRA1
Uniprot