Home » GFR alpha-1/GDNF R alpha-1 » GFR alpha-1/GDNF R alpha-1 Antibodies » GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

 (15 Publications)
 (8 Reviews)
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GFR alpha ‑1/GDNF R alpha ‑1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GFR alpha ‑1/GDNF R alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
GFR alpha ‑1/GDNF R alpha ‑1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GFR alpha ‑1/GDNF R alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) at ...read more
Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat GFR alpha ‑1/GDNF R alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) followed by ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF560
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Catalog# & Formulation Size Price
Carrier Free
AF560-SP
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GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat GFR alpha ‑1/GDNF R alpha ‑1
Asp25-Leu445
Accession # Q62997
Specificity
Detects rat GFR alpha ‑1/GDNF R alpha ‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human GFR alpha ‑1 is observed and less than 1% cross-reactivity with recombinant mouse GFR alpha ‑2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GFRA1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 8 Reviews rated 4.5
using
AF560 in the following applications:
Publications
Read Publications using
AF560 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

  • DKFZp313E1012
  • DKFZp686J0156
  • GDNF family receptor alpha 1
  • GDNF family receptor alpha-1
  • GDNF R alpha-1
  • GDNF receptor alpha-1
  • GDNFRAGFR-ALPHA-1
  • GDNFR-alpha-1
  • GDNFRMGC23045
  • GFR alpha1
  • GFR alpha-1
  • GFRA1
  • GFRa-1
  • GFR-alpha-1
  • Glial cell line-derived neurotrophic factor receptor alpha
  • GPI-linked anchor protein
  • PI-linked cell-surface accessory protein
  • RET ligand 1
  • RET1L
  • RETL1FLJ10561
  • TGF-beta related neurotrophic factor receptor 1
  • TGF-beta-related neurotrophic factor receptor 1
  • TRNR1FLJ31546

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and
‑2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an
N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GFR alpha-1/GDNF R alpha-1 Antibody (AF560)(15)

We have publications tested in 4 confirmed species: Mouse, Rat, Chicken, Porcine.

We have publications tested in 8 applications: ICC, IF, IHC, IHC - Not specified, IHC Paraffin-embedded, IHC-Fr, Neut, WB.


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ICC
(4)
IF
(1)
IHC
(1)
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(1)
IHC Paraffin-embedded
(2)
IHC-Fr
(1)
Neut
(2)
WB
(3)
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Mouse
(9)
Rat
(4)
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(1)
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(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF560 Applications Species
BA Niedenberg, CB Geyer Advanced immunostaining approaches to study early male germ cell development Stem Cell Res, 2018;0(0):. 2018 [PMID: 29475796] (IHC-Fr,, Mouse) IHC-Fr Mouse
JC Arnold, MA Cantu, EA Kasanga, VA Nejtek, EV Papa, N Bugnariu, MF Salvatore Aging-related limit of exercise efficacy on motor decline PLoS ONE, 2017;12(11):e0188538. 2017 [PMID: 29176896] (WB,, Rat) WB Rat
S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493. 2017 [PMID: 28270618] (Neut,, Mouse) Neut Mouse
Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors. Proc Natl Acad Sci U S A, 2015;112(15):E1946-55. 2015 [PMID: 25775569] (IHC - Not specified,, Mouse) IHC - Not specified Mouse
Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D PAX7 expression defines germline stem cells in the adult testis. J Clin Invest, 2014;124(9):3929-44. 2014 [PMID: 25133429] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Chakraborty P, Buaas F, Sharma M, Snyder E, de Rooij D, Braun R LIN28A marks the spermatogonial progenitor population and regulates its cyclic expansion. Stem Cells, 2014;32(4):860-73. 2014 [PMID: 24715688] (IF,, Mouse) IF Mouse
Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM). Mol Cell Neurosci, 2013;54(0):30-43. 2013 [PMID: 23262364] (WB,, Rat) WB Rat
Snyder EM, Small C, Griswold MD Retinoic acid availability drives the asynchronous initiation of spermatogonial differentiation in the mouse. Biol. Reprod., 2010;83(5):783-90. 2010 [PMID: 20650878] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Suzuki H, Sada A, Yoshida S, Saga Y The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3. Dev. Biol., 2009;336(2):222-31. 2009 [PMID: 19818747] (ICC,, Mouse) ICC Mouse
Linher K, Wu D, Li J Glial cell line-derived neurotrophic factor: an intraovarian factor that enhances oocyte developmental competence in vitro. Endocrinology, 2007;148(9):4292-301. 2007 [PMID: 17540724] (WB,, Porcine) WB Porcine
Show All 15 Publications.

Reviews for GFR alpha-1/GDNF R alpha-1 Antibody (AF560) (8) 4.58

Average Rating: 4.5
(Based on 8 reviews)
We have 8 reviews tested in 2 species: Mouse, Rat.

Reviews using AF560:
Filter by Applications
IF
(4)
IP
(1)
IHC-Fr
(1)
WB
(2)
All Applications
Filter by Species
Mouse
(5)
Rat
(3)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
 IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23012458
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23012458

Details

Detection NotesSee PMID 23012458

Comments

CommentsPublished: See PMID 23012458Independent review from antibodyresource.com
 
reviewed by:
Anonymous
 IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22711565
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22711565

Details

Detection NotesSee PMID 22711565

Comments

CommentsPublished: See PMID 22711565
Immunofluorescence GFR alpha-1/GDNF R alpha-1 AF560
Enlarge
reviewed by:
Anonymous
 IF Rat 12/18/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedRecombinant rat GFR alpha-1 expressed in 293 cells
SpeciesRat

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
 IP Rat 12/18/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedRat GFR alpha-1 expressed in 293 cells
SpeciesRat

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
 IHC-Fr Mouse 12/18/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedPFA-fixed cryosections of mouse brain
SpeciesMouse
 
reviewed by:
Anonymous
 IF Mouse 12/18/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHippocampal neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
 WB Rat 12/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedRecombinant rat GFR alpha-1 expressed in 293 cells
SpeciesRat

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
 WB Mouse 12/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedHippocampal neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/

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Recent Reviews

4.5
5
5
4
2
3
1
2
0
1
0
 
 Anonymous
01/08/2015
Application: IF
Species: Mouse
read this review
 
 Anonymous
01/08/2015
Application: IF
Species: Mouse
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Immunofluorescence GFR alpha-1/GDNF R alpha-1 AF560
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 Anonymous
12/18/2014
Application: IF
Species: Rat
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Bioinformatics

Gene Symbol GFRA1
Uniprot