GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

GFR alpha ‑1/GDNF R alpha ‑1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GFR alpha ‑1/GDNF R alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat GFR alpha ‑1/GDNF R alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GFR alpha ‑1/GDNF R alpha ‑1 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Rt
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat GFR alpha ‑1/GDNF R alpha ‑1
  Asp25-Leu445
  Accession # Q62997
• Specificity: Detects rat GFR alpha ‑1/GDNF R alpha ‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human GFR alpha ‑1 is observed and less than 1% cross-reactivity with recombinant mouse GFR alpha ‑2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GFRA1

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

• DKFZp313E1012
• DKFZp686J0156
• GDNF family receptor alpha 1
• GDNF family receptor alpha-1
• GDNF R alpha-1
• GDNF receptor alpha-1
• GDNFRAGFR-ALPHA-1
• GDNFR-alpha-1
• GDNFRMGC23045
• GFR alpha1
• GFR alpha-1
• GFRA1
• GFRa-1
• GFR-alpha-1
• Glial cell line-derived neurotrophic factor receptor alpha
• GPI-linked anchor protein
• PI-linked cell-surface accessory protein
• RET ligand 1
• RET1L
• RETL1FLJ10561
• TGF-beta related neurotrophic factor receptor 1
• TGF-beta-related neurotrophic factor receptor 1
• TRNR1FLJ31546

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and
‑2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an
N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFR alpha-1/GDNF R alpha-1 Antibody (AF560)(16)

Publications using AF560

• BP Hermann, K Cheng, A Singh, L Roa-De La, KN Mutoji, IC Chen, H Gilderslee, JD Lehle, M Mayo, B Westernstr, NC Law, MJ Oatley, EK Velte, BA Niedenberg, D Fritze, S Silber, CB Geyer, JM Oatley, JR McCarrey The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids Cell Rep, 2018;25(6):1650-1667.e8. 2018 [PMID: 30404016] (IHC-Fr,, Mouse)
• BA Niedenberg, CB Geyer Advanced immunostaining approaches to study early male germ cell development Stem Cell Res, 2018;0(0):. 2018 [PMID: 29475796] (IHC-Fr,, Mouse)
• JC Arnold, MA Cantu, EA Kasanga, VA Nejtek, EV Papa, N Bugnariu, MF Salvatore Aging-related limit of exercise efficacy on motor decline PLoS ONE, 2017;12(11):e0188538. 2017 [PMID: 29176896] (WB,, Rat)
• S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493. 2017 [PMID: 28270618] (Neut,, Mouse)
• Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors. Proc Natl Acad Sci U S A, 2015;112(15):E1946-55. 2015 [PMID: 25775569] (IHC - Not specified,, Mouse)
• Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D PAX7 expression defines germline stem cells in the adult testis. J Clin Invest, 2014;124(9):3929-44. 2014 [PMID: 25133429] (IHC Paraffin-embedded,, Mouse)
• Chakraborty P, Buaas F, Sharma M, Snyder E, de Rooij D, Braun R LIN28A marks the spermatogonial progenitor population and regulates its cyclic expansion. Stem Cells, 2014;32(4):860-73. 2014 [PMID: 24715688] (IF,, Mouse)
• Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM). Mol Cell Neurosci, 2013;54(0):30-43. 2013 [PMID: 23262364] (WB,, Rat)
• Snyder EM, Small C, Griswold MD Retinoic acid availability drives the asynchronous initiation of spermatogonial differentiation in the mouse. Biol. Reprod., 2010;83(5):783-90. 2010 [PMID: 20650878] (IHC Paraffin-embedded,, Mouse)
• Suzuki H, Sada A, Yoshida S, Saga Y The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3. Dev. Biol., 2009;336(2):222-31. 2009 [PMID: 19818747] (ICC,, Mouse)
• Linher K, Wu D, Li J Glial cell line-derived neurotrophic factor: an intraovarian factor that enhances oocyte developmental competence in vitro. Endocrinology, 2007;148(9):4292-301. 2007 [PMID: 17540724] (WB,, Porcine)
• Taylor AR, Gifondorwa DJ, Newbern JM, Robinson MB, Strupe JL, Prevette D, Oppenheim RW, Milligan CE Astrocyte and muscle-derived secreted factors differentially regulate motoneuron survival. J. Neurosci., 2007;27(3):634-44. 2007 [PMID: 17234595] (Neut,, Chicken)
• Igarashi Y, Aita M, Suzuki A, Nandasena T, Kawano Y, Nozawa-Inoue K, Maeda T Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings. Neurosci. Lett., 2007;412(3):222-6. 2007 [PMID: 17178438] (IHC,, Rat)
• Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo. J. Neurosci., 2006;26(33):8588-99. 2006 [PMID: 16914685] (ICC,, Mouse)
• Wanigasekara Y, Keast JR Nerve growth factor, glial cell line-derived neurotrophic factor and neurturin prevent semaphorin 3A-mediated growth cone collapse in adult sensory neurons. Neuroscience, 2006;142(2):369-79. 2006 [PMID: 16876331] (ICC,, Rat)
• Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM, Choi DW, Snider BJ Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells. Exp. Neurol., 2005;191(1):65-76. 2005 [PMID: 15589513] (ICC,, Mouse)

Reviews for GFR alpha-1/GDNF R alpha-1 Antibody (AF560) (8)

Average Rating: 4.5

(Based on 8 reviews)

We have 8 reviews tested in 2 species: Mouse, Rat.

reviewed by: Anonymous IF Mouse 01/08/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23012458
• Species: Mouse

Blocking

• Blocking Details: See PMID 23012458

Details

• Detection Notes: See PMID 23012458

Comments

• Comments: Published: See PMID 23012458Independent review from antibodyresource.com

reviewed by: Anonymous IF Mouse 01/08/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22711565
• Species: Mouse

Blocking

• Blocking Details: See PMID 22711565

Details

• Detection Notes: See PMID 22711565

Comments

• Comments: Published: See PMID 22711565

reviewed by: Anonymous IF Rat 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Recombinant rat GFR alpha-1 expressed in 293 cells
• Species: Rat

Blocking

• Blocking Details: n/a

Details

• Detection Notes: n/a

Comments

• Comments: Five-star review on pAbmAbs http://pabmabs.com/

reviewed by: Anonymous IP Rat 12/18/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: Rat GFR alpha-1 expressed in 293 cells
• Species: Rat

Blocking

• Blocking Details: n/a

Details

• Detection Notes: n/a

Comments

• Comments: Five-star review on pAbmAbs http://pabmabs.com/

reviewed by: Anonymous IHC-Fr Mouse 12/18/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: PFA-fixed cryosections of mouse brain
• Species: Mouse

reviewed by: Anonymous IF Mouse 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Hippocampal neurons
• Species: Mouse

Blocking

• Blocking Details: n/a

Details

• Detection Notes: n/a

Comments

• Comments: Five-star review on pAbmAbs http://pabmabs.com/

reviewed by: Anonymous WB Rat 12/18/2014

Summary

• Application: Western Blot
• Sample Tested: Recombinant rat GFR alpha-1 expressed in 293 cells
• Species: Rat

Blocking

• Blocking Details: n/a

Details

• Detection Notes: n/a

Comments

• Comments: Five-star review on pAbmAbs http://pabmabs.com/

reviewed by: Anonymous WB Mouse 12/18/2014

Summary

• Application: Western Blot
• Sample Tested: Hippocampal neurons
• Species: Mouse

Blocking

• Blocking Details: n/a

Details

• Detection Notes: n/a

Comments

• Comments: Five-star review on pAbmAbs http://pabmabs.com/

Bioinformatics

• Gene Symbol: GFRA1
• Uniprot:
  • Q62997()