Persephin Antibody (275508) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
275508
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Persephin Antibody (275508) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Persephin
Ala61-Gly156
Accession # O60542
Specificity
Detects human Persephin in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) AgRP, rhArtemin, rh beta IG-H3, rhCripto-1, recombinant drosophila Decapentaplegic, rhGDNF, rhLAP, rhLatent TGF-beta 1, rhLefty-A, rhMIS, rhNeurturin, recombinant mouse (rh) Nodal, rmPersephin, rhTGF-alpha, rhTGF-beta 1, rhTGF-beta 1.2, rhTGF-beta 2, rhTGF-beta 3, or recombinant amphibian TGF-beta 5 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PSPN
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Persephin Antibody (275508) [Unconjugated]

  • Persephin
  • PSP
  • PSPN

Background

Persephin is a secreted protein belonging to the glial cell line-derived neurotrophic factor (GDNF) family of the TGF-beta superfamily. It shares 38-46% amino acid (aa) identity with family members GDNF, neurturin and artemin. Persephin is expressed at very low levels in most tissues (1). The 156 aa, 10-12 kDa mature protein contains a signal sequence, a pro-domain and a 96 aa mature sequence with several cysteines that are conserved among family members. It circulates as an unglycosylated disulfide-linked homodimer. Mature human Persephin shares 81% and 80%, 89%, and 87% amino acid sequence identity with mouse, rat, bovine and canine Persephin, respectively. Like other GDNF family members, Persephin acts through engagement of GRF alpha 4, a glycosylphosphatidylinositol (GPI)-linked GDNF receptor family (GRF) member that signals through the receptor tyrosine kinase RET. Persephin is reported to promote both the survival and growth of central dopaminergic and motor neurons, and kidney development (1). These effects are correlated with the expression patterns of GFR alpha 4, and RET (2, 3). Functional GFR alpha 4 isoforms are found only in thyroid, adrenal medulla and portions of the central nervous system, and include GPI-linked, transmembrane and soluble forms (3, 4). In vitro, Persephin promotes survival only in neurons which coexpress GPI-linked GFR alpha 4 with RET (2, 5). This effect does not show a strong correlation to the recruitment of RET in lipid rafts seen with other GDNF family members (6). Disruption of the Persephin gene results in mice that are morphologically normal but have more damage and less effective repair after central nervous system insult that stimulates a stroke. Microinjection of Persephin prior to treatment protects against damage in both wild-type and mutant mouse brains, but surprisingly, high doses of Persephin are detrimental (7).

  1. Milbrandt, J. et al. (1998) Neuron 20:245.
  2. Lindahl, M. et al. (2001) J. Biol. Chem. 276:9344.
  3. Lindahl, M. et al. (2000) Mol. Cell. Neurosci. 15:522.
  4. Akerud, P. et al. (2002) Mol. Cell. Neurosci. 21:205.
  5. Enokido, Y. et al. (1998) Current Biol. 8:1019.
  6. Yang, J. et al. (2004) FEBS Lett. 569:267.
  7. Tomac, A.C. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9521.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PSPN
Uniprot