Neurocan Antibody [Biotin]

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Neurocan Antibody [Biotin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Neurocan
  Asp23-Asp637
  Accession # NP_031815
• Specificity: Detects mouse and rat Neurocan in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Ncan
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurocan Antibody [Biotin]

• chondroitin sulfate proteoglycan 3 (neurocan)
• Chondroitin sulfate proteoglycan 3
• CSPG3
• CSPG3neurocan core protein
• FLJ44681
• NCAN
• NEUR
• neurocan proteoglycan
• Neurocan

Background

Neurocan is a 220 kDa nervous tissue-specific chondroitin sulfate proteoglycan member of the aggrecan/versican proteoglycan family (1). Mouse Neurocan is synthesized as a 1268 amino acid (aa) precursor that contains a 22 aa signal sequence and a 1246 aa mature chain. The mature chain contains one Ig-like V-type domain (aa 37-157), two Link domains (aa 159-254 and 258-356), two EGF-like domains (aa 960-996 and 998-1034), one C-type lectin-like domain (aa 1036-1165), one Sushi domain (aa 1165-1224), and five potential sites for N-linked glycosylation. Mature mouse Neurocan is 90% and 66% aa identical to mature rat and human Neurocan, respectively. Neurocan binds with high affinity to the cell adhesion molecules (CAM) Ng-CAM and N-CAM to inhibit Neuronal adhesion and neurite growth (2-3). In the developing rat retina, the expression of Neurocan is regulated both temporally and spatially, which suggests that it may play a role in the differentiation of and neural network formation of the mammalian retina (1). Injury to the CNS leads to permanent loss of function due to the inability of severed nerve fibers to regenerate back to their targets (4). The lack of CNS repair is attributed in part to the extracellular matrix chondroitin sulfate proteoglycans, such as Neurocan, which are produced by activated glial cells post-injury (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurocan Antibody (BAF5800)(3)

Publications using BAF5800

• Craig S. Pearson, Andrea G. Solano, Sharada M. Tilve, Caitlin P. Mencio, Keith R. Martin, Herbert M. Geller Spatiotemporal distribution of chondroitin sulfate proteoglycans after optic nerve injury in rodents Experimental Eye Research 2020-01-01 [PMID: 31705897]
• Jae-Hyuk Yi, Yasuhiro Katagiri, Bala Susarla, David Figge, Aviva J. Symes, Herbert M. Geller Alterations in sulfated chondroitin glycosaminoglycans following controlled cortical impact injury in mice The Journal of Comparative Neurology 2012-10-15 [PMID: 22628090]
• Jae-Hyuk Yi, Yasuhiro Katagiri, Bala Susarla, David Figge, Aviva J. Symes, Herbert M. Geller Alterations in sulfated chondroitin glycosaminoglycans following controlled cortical impact injury in mice The Journal of Comparative Neurology 2012-10-15 [PMID: 22628090] (Immunohistochemistry, Mouse)

Bioinformatics

• Gene Symbol: Ncan
• Uniprot:
  • NP_031815()