Western blot shows lysates of human brain (cerebellum) tissue and human brain (motor cortex) tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Rat Brevican Antigen Affinity-purified Polyclonal ...read more
Brevican was detected in immersion fixed rat cortical stem cells differentiated for 7 days by growth factor withdrawal using Sheep Anti-Human/Rat Brevican Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Brevican was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human/Rat Brevican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4009) at 5 µg/mL ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Brevican Antibody [Unconjugated]
BEHABbrevican core protein
Brain-enriched hyaluronan-binding protein
Chondroitin sulfate proteoglycan 7
chondroitin sulfate proteoglycan BEHAB
chondroitin sulfate proteoglycan BEHAB/brevican
Brevican, also called BEHAB, is a secreted member of the the lectican family of proteoglycans that share a common domain structure (1). Brevican contains an Ig‑like V-set domain, two link domains, a Glu-rich region, a central region with glycosaminoglycan (GAG) modifications, an EGF-like domain, a C-type lectin domain, and a C-terminal Sushi/CRP-like domain (2). Brevican is restricted to the CNS and is expressed by astrocytes, oligodendrocytes, and neurons (3‑7). A GPI-anchored alternate splice form exists that is truncated following the central (GAG) region (2, 8). Brevican is cleaved by multiple proteases and exists in a number of distinct fragments (5, 9, 10). Full-length brevican consists of a 97 kDa core protein with up to approximately 100 kDa of attached chondroitin sulfate but not heparan sulfate chains (4, 7, 11, 12). Brevican associates with the extracellular matrix, perineuronal nets, and astrocyte cell surfaces by means of its chondroitin sulfate, GPI anchor, hyaluronic acid-binding link domains, and the core protein (4, 7, 8, 13). The secreted isoform is dominant during brain development and is up‑regulated in astrocytes following brain injury (2, 14). In human and rat, an under-glycosylated form of brevican is up‑regulated in highly aggressive glioma but not in low-grade glioma or other brain pathologies (15, 16). In mouse and rat, levels of an ADAMTS4-generated 55 kDa N-terminal fragment increase during remodeling after excitotoxic injury (11, 12). Human brevican shares 90%, 80%, and 80% aa sequence identity with bovine, mouse, and rat brevican, respectively. Within the Ig-like and two link domains, brevican shares 45%‑51% aa sequence identity with aggrecan, neurocan, and versican.
Viapiano, M.S. and R.T. Matthews (2006) Trends Mol. Med. 12:488.
Gary, S.C. et al. (2000) Gene 256:139.
Jaworski, D.M. et al. (1994) J. Cell Biol. 125:495.
Seidenbecher, C.I. et al. (1995) J. Biol. Chem. 270:27206.
Hamel, M.G. et al. (2005) J. Neurochem. 93:1533.
Ogawa, T. et al. (2001) J. Comp. Neurol. 432:285
Yamada, H. et al. (1997) J. Neurosci. 17:7784.
Seidenbecher, C.I. et al. (2002) J. Neurochem. 83:738.
Matthews, R.T. et al. (2000) J. Biol. Chem. 275:22695.
Nakamura, H. et al. (2000) J. Biol. Chem. 275:38885.
Mayer, J. et al. (2005) BMC Neurosci. 6:52.
Yuan, W. et al. (2002) Neuroscience 114:1091.
Deepa, S.S. et al. (2006) J. Biol. Chem. 281:17789.
Jaworski, D.M. et al. (1999) Exp. Neurol. 157:327.
Viapiano, M.S. et al. (2005) Cancer Res. 65:6726.
Viapiano, M.S. et al. (2003) J. Biol. Chem. 278:33239.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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