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Brevican Antibody [Unconjugated]

Images

 
Western blot shows lysates of human brain (cerebellum) tissue and human brain (motor cortex) tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Rat Brevican Antigen Affinity-purified Polyclonal ...read more
Brevican was detected in immersion fixed rat cortical stem cells differentiated for 7 days by growth factor withdrawal using Sheep Anti-Human/Rat Brevican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4009) ...read more
Brevican was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human/Rat Brevican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4009) at 5 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IP, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Brevican Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Brevican
Asp23-Pro911
Accession # AAH27971
Specificity
Detects human Brevican in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Brevican is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
BCAN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF4009 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brevican Antibody [Unconjugated]

  • ALPBRE
  • BCAN
  • BEHAB
  • BEHABbrevican core protein
  • Brain-enriched hyaluronan-binding protein
  • Brevican
  • Chondroitin sulfate proteoglycan 7
  • chondroitin sulfate proteoglycan BEHAB
  • chondroitin sulfate proteoglycan BEHAB/brevican
  • CSPG7
  • CSPG7brevican proteoglycan
  • MGC13038

Background

Brevican, also called BEHAB, is a secreted member of the the lectican family of proteoglycans that share a common domain structure (1). Brevican contains an Ig‑like V-set domain, two link domains, a Glu-rich region, a central region with glycosaminoglycan (GAG) modifications, an EGF-like domain, a C-type lectin domain, and a C-terminal Sushi/CRP-like domain (2). Brevican is restricted to the CNS and is expressed by astrocytes, oligodendrocytes, and neurons (3‑7). A GPI-anchored alternate splice form exists that is truncated following the central (GAG) region (2, 8). Brevican is cleaved by multiple proteases and exists in a number of distinct fragments (5, 9, 10). Full-length brevican consists of a 97 kDa core protein with up to approximately 100 kDa of attached chondroitin sulfate but not heparan sulfate chains (4, 7, 11, 12). Brevican associates with the extracellular matrix, perineuronal nets, and astrocyte cell surfaces by means of its chondroitin sulfate, GPI anchor, hyaluronic acid-binding link domains, and the core protein (4, 7, 8, 13). The secreted isoform is dominant during brain development and is up‑regulated in astrocytes following brain injury (2, 14). In human and rat, an under-glycosylated form of brevican is up‑regulated in highly aggressive glioma but not in low-grade glioma or other brain pathologies (15, 16). In mouse and rat, levels of an ADAMTS4-generated 55 kDa N-terminal fragment increase during remodeling after excitotoxic injury (11, 12). Human brevican shares 90%, 80%, and 80% aa sequence identity with bovine, mouse, and rat brevican, respectively. Within the Ig-like and two link domains, brevican shares 45%‑51% aa sequence identity with aggrecan, neurocan, and versican.
  1. Viapiano, M.S. and R.T. Matthews (2006) Trends Mol. Med. 12:488. 
  2. Gary, S.C. et al. (2000) Gene 256:139. 
  3. Jaworski, D.M. et al. (1994) J. Cell Biol. 125:495. 
  4. Seidenbecher, C.I. et al. (1995) J. Biol. Chem. 270:27206. 
  5. Hamel, M.G. et al. (2005) J. Neurochem. 93:1533. 
  6. Ogawa, T. et al. (2001) J. Comp. Neurol. 432:285  
  7. Yamada, H. et al. (1997) J. Neurosci. 17:7784.  
  8. Seidenbecher, C.I. et al. (2002) J. Neurochem. 83:738.
  9. Matthews, R.T. et al. (2000) J. Biol. Chem. 275:22695.
  10. Nakamura, H. et al. (2000) J. Biol. Chem. 275:38885.
  11. Mayer, J. et al. (2005) BMC Neurosci. 6:52.
  12. Yuan, W. et al. (2002) Neuroscience 114:1091.
  13. Deepa, S.S. et al. (2006) J. Biol. Chem. 281:17789.
  14. Jaworski, D.M. et al. (1999) Exp. Neurol. 157:327.
  15. Viapiano, M.S. et al. (2005) Cancer Res. 65:6726.
  16. Viapiano, M.S. et al. (2003) J. Biol. Chem. 278:33239.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Brevican Antibody (AF4009)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ELISA Development, IHC, IHC-Fr, Western Blot.


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ELISA Development
(1)
IHC
(2)
IHC-Fr
(2)
Western Blot
(1)
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Human
(1)
Mouse
(4)
Rat
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF4009 Applications Species
José Javier Miguel-Hidalgo, Erik Hearn, Mohadetheh Moulana, Khunsa Saleem, Austin Clark, Maggie Holmes et al. Reduced length of nodes of Ranvier and altered proteoglycan immunoreactivity in prefrontal white matter in major depressive disorder and chronically stressed rats Sci Rep 2023-09-29 [PMID: 37775676]
Hiroshi Ueno, Yu Takahashi, Shinji Murakami, Kenta Wani, Tetsuji Miyazaki, Yosuke Matsumoto et al. Component-specific reduction in perineuronal nets in senescence-accelerated mouse strains IBRO Neuroscience Reports 2023-06-01 [PMID: 37388498]
L Höhn, W Hu beta ler, A Richter, KH Smalla, AM Birkl-Toeg, C Birkl, S Vielhaber, SL Leber, ED Gundelfing, J Haybaeck, S Schreiber, CI Seidenbech Extracellular Matrix Changes in Subcellular Brain Fractions and Cerebrospinal Fluid of Alzheimer's Disease Patients International Journal of Molecular Sciences, 2023-03-14;24(6):. 3/14/2023 [PMID: 36982604]
A Ritok, P Kiss, A Zaher, E Wolf, L Ducza, T Bacskai, C Matesz, B Gaal Distribution and postnatal development of chondroitin sulfate proteoglycans in the perineuronal nets of cholinergic motoneurons innervating extraocular muscles Scientific Reports, 2022-12-14;12(1):21606. 2022-12-14 [PMID: 36517521] (IHC, Mouse) IHC Mouse
W Hu beta ler, L Höhn, C Stolz, S Vielhaber, C Garz, FC Schmitt, ED Gundelfing, S Schreiber, CI Seidenbech Brevican and Neurocan Cleavage Products in the Cerebrospinal Fluid - Differential Occurrence in ALS, Epilepsy and Small Vessel Disease Frontiers in Cellular Neuroscience, 2022-04-11;16(0):838432. 2022-04-11 [PMID: 35480959] (ELISA Development, Human) ELISA Development Human
AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 2020-07-31;10(1):12912. 2020-07-31 [PMID: 32737331] (Western Blot, Mouse) Western Blot Mouse
Andrea Hunyadi, Botond Gaál, Clara Matesz, Zoltan Meszar, Markus Morawski, Katja Reimann et al. Distribution and classification of the extracellular matrix in the olfactory bulb Brain Structure and Function 2020-01-01 [PMID: 31858237]
Craig S. Pearson, Andrea G. Solano, Sharada M. Tilve, Caitlin P. Mencio, Keith R. Martin, Herbert M. Geller Spatiotemporal distribution of chondroitin sulfate proteoglycans after optic nerve injury in rodents Experimental Eye Research 2020-01-01 [PMID: 31705897]
H Ueno, S Suemitsu, S Murakami, N Kitamura, K Wani, Y Matsumoto, M Okamoto, T Ishihara Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices IBRO Rep, 2018-11-28;6(0):1-17. 2018-11-28 [PMID: 30582064] (IHC-Fr, Mouse) IHC-Fr Mouse
SF Irvine, JCF Kwok Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones Int J Mol Sci, 2018-04-12;19(4):. 2018-04-12 [PMID: 29649136] (IHC, Rat) IHC Rat
Show All 11 Publications.

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Bioinformatics

Gene Symbol BCAN
Uniprot