Contactin‑2/TAG1 was detected in immersion fixed frozen sections of mouse embryo (E13) using Goat Anti-Human/Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) at 15 µg/mL ...read more
Simple Western lane view shows lysates of mouse spinal cord tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑2/TAG1 at approximately 162 kDa (as indicated) using 10 µg/mL of Goat ...read more
Western blot shows lysates of human cerebellum tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin‑2/TAG1 Antigen ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Contactin-2/TAG1 Antibody [Unconjugated]
Axonal glycoprotein TAG-1
axonin-1 cell adhesion molecule
contactin 2 (axonal)
contactin 2 (transiently expressed)
Transient axonal glycoprotein 1
transiently-expressed axonal glycoprotein
Contactin-2 (CNTN2), also called TAG1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1-3). Mouse Contactin-2 cDNA encodes a 30 amino acid (aa) signal peptide, a 984 aa mature secreted protein with 6 Ig-like domains followed by 4 fibronectin type III-like repeats, and a 26 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature mouse Contactin-2 shares approximately 93%, 97%, and 77% aa sequence identity with human, rat, and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3-5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3-6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits contactin-associated protein 2 (caspr2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7-10). The axonal Contactin-2 interacts in cis with caspr2 and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).
Wolfer, D. and R.J. Giger (1994) Swissprot Accession # Q61330.
Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
Karagogeos, D. (2003) Front. Biosci. 8:s1304.
Liu, Y. and M.C. Halloran (2005) J. Neurosci. 25:10556.
Denaxa, M. et al. (2005) Dev. Biol. 288:87.
Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
Traka, M. et al. (2002) J. Neurosci. 22:3016.
Poliak, S. and E. Peles (2003) Nat. Rev. Neurosci. 4:968.
Traka, M. et al. (2003) J. Cell Biol. 162:1161.
Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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