Contactin-2/TAG1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse spinal cord tissue and rat embryonic cortical neuron/glial cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Contactin‑2/TAG1 Polyclonal Antibody (Catalog # ...read more
Contactin‑2/TAG1 was detected in immersion fixed frozen sections of mouse embryo (E13) using Goat Anti-Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) at 15 µg/mL ...read more
Simple Western lane view shows lysates of mouse spinal cord tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑2/TAG1 at approximately 162 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Contactin-2/TAG1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Contactin-2/TAG1
Gln31-Ser1014
Accession # Q61330
Specificity
Detects mouse and rat Contactin-2 in direct ELISAs and Western blots. In direct ELISAs, 20% cross-reactivity with recombinant human (rh) Contactin‑2 is observed and 2% cross-reactivity with recombinant mouse (rm) Contactin‑3, rhContactin‑4, rmContactin‑4, rhContactin‑5, and rmContactin‑6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CNTN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF4439 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-2/TAG1 Antibody [Unconjugated]

  • Axonal glycoprotein TAG-1
  • axonin-1 cell adhesion molecule
  • Axonin-1
  • AXT
  • CNTN2
  • contactin 2 (axonal)
  • contactin 2 (transiently expressed)
  • Contactin2
  • Contactin-2
  • DKFZp781D102
  • FLJ37193
  • MGC157722
  • TAG1
  • TAG-1
  • TAX
  • TAX1
  • TAX-1
  • TAX1FLJ42746
  • Transient axonal glycoprotein 1
  • transiently-expressed axonal glycoprotein

Background

Contactin-2 (CNTN2), also called TAG1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1-3). Mouse Contactin-2 cDNA encodes a 30 amino acid (aa) signal peptide, a 984 aa mature secreted protein with 6 Ig-like domains followed by 4 fibronectin type III-like repeats, and a 26 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature mouse Contactin-2 shares approximately 93%, 97%, and 77% aa sequence identity with human, rat, and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3-5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3-6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits contactin-associated protein 2 (caspr2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7-10). The axonal Contactin-2 interacts in cis with caspr2 and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).

  1. Wolfer, D. and R.J. Giger (1994) Swissprot Accession # Q61330.
  2. Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
  3. Karagogeos, D. (2003) Front. Biosci. 8:s1304.
  4. Liu, Y. and M.C. Halloran (2005) J. Neurosci. 25:10556.
  5. Denaxa, M. et al. (2005) Dev. Biol. 288:87.
  6. Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
  7. Traka, M. et al. (2002) J. Neurosci. 22:3016.
  8. Poliak, S. and E. Peles (2003) Nat. Rev. Neurosci. 4:968.
  9. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
  10. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Contactin-2/TAG1 Antibody (AF4439)(7)

We have publications tested in 2 confirmed species: Mouse, Primate.

We have publications tested in 4 applications: ICC, IHC, IHC OCT-embedded, WB.


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ICC
(1)
IHC
(1)
IHC OCT-embedded
(1)
WB
(4)
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Mouse
(6)
Primate
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Showing Publications 1 - 7 of 7.
Publications using AF4439 Applications Species
N Hamada, I Iwamoto, H Tabata, KI Nagata MUNC18-1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development Acta Neuropathol Commun, 2017;5(1):92. 2017 [PMID: 29191246] (ICC,, Primate) ICC Primate
K Onishi, A Uyeda, M Shida, T Hirayama, T Yagi, N Yamamoto, N Sugo Genome Stability by DNA polymerase ? in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development J. Neurosci., 2017;0(0):. 2017 [PMID: 28765330] (IHC,, Mouse) IHC Mouse
S Bhattachar, M Bhakta, NV Munshi Phenotypically silent Cre recombination within the postnatal ventricular conduction system PLoS ONE, 2017;12(3):e0174517. 2017 [PMID: 28358866] (WB,, Mouse) WB Mouse
NOVA2-mediated RNA regulation is required for axonal pathfinding during development Elife, 2016;5(0):. 2016 [PMID: 27223325] (IHC OCT-embedded,, Mouse) IHC OCT-embedded Mouse
Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease. EMBO Mol Med, 2015;7(2):175-89. 2015 [PMID: 25592972] (WB,, Mouse) WB Mouse
Gautam, Vivek, D'Avanzo, Carla, Hebisch, Matthias, Kovacs, Dora M, Kim, Doo Yeon BACE1 activity regulates cell surface contactin-2 levels. Mol Neurodegener, 2014;9(0):4. 2014 [PMID: 24405708] (WB,, Mouse) WB Mouse
Kuhn PH, Koroniak K, Hogl S, Colombo A, Zeitschel U, Willem M, Volbracht C, Schepers U, Imhof A, Hoffmeister A, Haass C, Rossner S, Brase S, Lichtenthaler SF Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons. EMBO J., 2012;31(14):3157-68. 2012 [PMID: 22728825] (WB,, Mouse) WB Mouse

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Bioinformatics

Gene Symbol CNTN2
Entrez
Uniprot