Contactin-2/TAG1 Antibody [Unconjugated]

Western blot shows lysates of human cerebellum tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Contactin‑2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Contactin‑2/TAG1 was detected in immersion fixed frozen sections of mouse embryo (E13) using Goat Anti-Human/Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells in proximity to ribs. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of mouse spinal cord tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑2/TAG1 at approximately 162 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Contactin-2/TAG1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Contactin-2/TAG1
  Gln31-Ser1014
  Accession # Q61330
• Specificity: Detects mouse and rat Contactin-2 in direct ELISAs. Detects human, mouse, and rat Contactin-2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CNTN2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-2/TAG1 Antibody [Unconjugated]

• Axonal glycoprotein TAG-1
• axonin-1 cell adhesion molecule
• Axonin-1
• AXT
• CNTN2
• contactin 2 (axonal)
• contactin 2 (transiently expressed)
• Contactin2
• Contactin-2
• DKFZp781D102
• FLJ37193
• MGC157722
• TAG1
• TAG-1
• TAX
• TAX1
• TAX-1
• TAX1FLJ42746
• Transient axonal glycoprotein 1
• transiently-expressed axonal glycoprotein

Background

Contactin-2 (CNTN2), also called TAG1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1-3). Mouse Contactin-2 cDNA encodes a 30 amino acid (aa) signal peptide, a 984 aa mature secreted protein with 6 Ig-like domains followed by 4 fibronectin type III-like repeats, and a 26 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature mouse Contactin-2 shares approximately 93%, 97%, and 77% aa sequence identity with human, rat, and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3-5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3-6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits contactin-associated protein 2 (caspr2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7-10). The axonal Contactin-2 interacts in cis with caspr2 and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K⁺ channels in the juxtaparanodes (9).

1. Wolfer, D. and R.J. Giger (1994) Swissprot Accession # Q61330.
2. Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
3. Karagogeos, D. (2003) Front. Biosci. 8:s1304.
4. Liu, Y. and M.C. Halloran (2005) J. Neurosci. 25:10556.
5. Denaxa, M. et al. (2005) Dev. Biol. 288:87.
6. Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
7. Traka, M. et al. (2002) J. Neurosci. 22:3016.
8. Poliak, S. and E. Peles (2003) Nat. Rev. Neurosci. 4:968.
9. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
10. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Contactin-2/TAG1 Antibody (AF4439)(12)

Publications using AF4439

• L Cotellessa, F Marelli, P Duminuco, M Adamo, GE Papadakis, L Bartoloni, N Sato, M Lang-Murit, A Troendle, WS Dhillo, A Morelli, G Guarnieri, N Pitteloud, L Persani, M Bonomi, P Giacobini, V Vezzoli Defective Jagged1 signaling impacts GnRH development and contributes to congenital hypogonadotropic hypogonadism JCI Insight, 2023;0(0):. 2023-01-01 [PMID: 36729644] (IHC, Human)
• R Jorgensen, M Katta, J Wolfe, DF Leach, B Lavelle, J Chun, LD Wilsbacher Deletion of Sphingosine 1-Phosphate receptor 1 in cardiomyocytes during development leads to abnormal ventricular conduction and fibrosis Physiological Reports, 2021;9(19):e15060. 2021-01-01 [PMID: 34618403] (Western Blot, Mouse)
• A Ribeiro da, EA Neri, LT Turaça, R Dariolli, MH Fonseca-Al, A Santos-Mir, D Roman-Camp, G Venturini, JE Krieger NOTCH1 is critical for fibroblast-mediated induction of cardiomyocyte specialization into ventricular conduction system-like cells in vitro Sci Rep, 2020;10(1):16163. 2020-01-01 [PMID: 32999360] (ICC, Rat)
• S Skarlatou, C Hérent, E Toscano, CS Mendes, J Bouvier, N Zampieri Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection Cell Rep, 2020;31(10):107741. 2020-01-01 [PMID: 32521266] (IHC, Mouse)
• DJ Flores, T Duong, LO Brandenber, A Mitra, A Shirali, JC Johnson, D Springer, A Noguchi, ZX Yu, SN Ebert, A Ludwig, BC Knollmann, MD Levin, K Pfeifer Conditional ablation and conditional rescue models for Casq2 elucidate the role of development and of cell type specific expression of Casq2 in the CPVT2 phenotype Hum. Mol. Genet., 2018;0(0):. 2018-01-01 [PMID: 29452352] (IHC-Fr, Mouse)
• N Hamada, I Iwamoto, H Tabata, KI Nagata MUNC18-1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development Acta Neuropathol Commun, 2017;5(1):92. 2017-01-01 [PMID: 29191246] (ICC, Primate)
• K Onishi, A Uyeda, M Shida, T Hirayama, T Yagi, N Yamamoto, N Sugo Genome Stability by DNA polymerase ? in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development J. Neurosci., 2017;0(0):. 2017-01-01 [PMID: 28765330] (IHC, Mouse)
• S Bhattachar, M Bhakta, NV Munshi Phenotypically silent Cre recombination within the postnatal ventricular conduction system PLoS ONE, 2017;12(3):e0174517. 2017-01-01 [PMID: 28358866] (Western Blot, Mouse)
• NOVA2-mediated RNA regulation is required for axonal pathfinding during development Elife, 2016;5(0):. 2016-01-01 [PMID: 27223325] (IHC, Mouse)
• Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease. EMBO Mol Med, 2015;7(2):175-89. 2015-01-01 [PMID: 25592972] (Western Blot, Mouse)
• Gautam , Vivek, D'Avanzo , Carla, Hebisch , Matthias, Kovacs , Dora M, Kim , Doo Yeon BACE1 activity regulates cell surface contactin-2 levels. Mol Neurodegener, 2014;9(0):4. 2014-01-01 [PMID: 24405708] (Western Blot, Mouse)
• Kuhn PH, Koroniak K, Hogl S, Colombo A, Zeitschel U, Willem M, Volbracht C, Schepers U, Imhof A, Hoffmeister A, Haass C, Rossner S, Brase S, Lichtenthaler SF Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons. EMBO J., 2012;31(14):3157-68. 2012-01-01 [PMID: 22728825] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CNTN2
• Uniprot:
  • Q61330()