Contactin-2/TAG1 Antibody [Unconjugated]


Western blot shows lysates of human cerebellum tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin‑2/TAG1 Antigen more
Contactin‑2/TAG1 was detected in immersion fixed frozen sections of mouse embryo (E13) using Goat Anti-Human/Mouse/Rat Contactin‑2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) at 15 µg/mL more
Simple Western lane view shows lysates of mouse spinal cord tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑2/TAG1 at approximately 162 kDa (as indicated) using 10 µg/mL of Goat more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC

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Contactin-2/TAG1 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse Contactin-2/TAG1
Accession # Q61330
Detects mouse and rat Contactin-2 in direct ELISAs. Detects human, mouse, and rat Contactin-2 in Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF4439 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-2/TAG1 Antibody [Unconjugated]

  • Axonal glycoprotein TAG-1
  • axonin-1 cell adhesion molecule
  • Axonin-1
  • AXT
  • CNTN2
  • contactin 2 (axonal)
  • contactin 2 (transiently expressed)
  • Contactin2
  • Contactin-2
  • DKFZp781D102
  • FLJ37193
  • MGC157722
  • TAG1
  • TAG-1
  • TAX
  • TAX1
  • TAX-1
  • TAX1FLJ42746
  • Transient axonal glycoprotein 1
  • transiently-expressed axonal glycoprotein


Contactin-2 (CNTN2), also called TAG1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1-3). Mouse Contactin-2 cDNA encodes a 30 amino acid (aa) signal peptide, a 984 aa mature secreted protein with 6 Ig-like domains followed by 4 fibronectin type III-like repeats, and a 26 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature mouse Contactin-2 shares approximately 93%, 97%, and 77% aa sequence identity with human, rat, and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3-5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3-6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits contactin-associated protein 2 (caspr2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7-10). The axonal Contactin-2 interacts in cis with caspr2 and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).

  1. Wolfer, D. and R.J. Giger (1994) Swissprot Accession # Q61330.
  2. Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
  3. Karagogeos, D. (2003) Front. Biosci. 8:s1304.
  4. Liu, Y. and M.C. Halloran (2005) J. Neurosci. 25:10556.
  5. Denaxa, M. et al. (2005) Dev. Biol. 288:87.
  6. Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
  7. Traka, M. et al. (2002) J. Neurosci. 22:3016.
  8. Poliak, S. and E. Peles (2003) Nat. Rev. Neurosci. 4:968.
  9. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
  10. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Contactin-2/TAG1 Antibody (AF4439)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, Western Blot.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF4439 Applications Species
L Cotellessa, F Marelli, P Duminuco, M Adamo, GE Papadakis, L Bartoloni, N Sato, M Lang-Murit, A Troendle, WS Dhillo, A Morelli, G Guarnieri, N Pitteloud, L Persani, M Bonomi, P Giacobini, V Vezzoli Defective Jagged1 signaling impacts GnRH development and contributes to congenital hypogonadotropic hypogonadism JCI Insight, 2023;0(0):. 2023-01-01 [PMID: 36729644] (IHC, Human) IHC Human
R Jorgensen, M Katta, J Wolfe, DF Leach, B Lavelle, J Chun, LD Wilsbacher Deletion of Sphingosine 1-Phosphate receptor 1 in cardiomyocytes during development leads to abnormal ventricular conduction and fibrosis Physiological Reports, 2021;9(19):e15060. 2021-01-01 [PMID: 34618403] (Western Blot, Mouse) Western Blot Mouse
A Ribeiro da, EA Neri, LT Turaça, R Dariolli, MH Fonseca-Al, A Santos-Mir, D Roman-Camp, G Venturini, JE Krieger NOTCH1 is critical for fibroblast-mediated induction of cardiomyocyte specialization into ventricular conduction system-like cells in vitro Sci Rep, 2020;10(1):16163. 2020-01-01 [PMID: 32999360] (ICC, Rat) ICC Rat
S Skarlatou, C Hérent, E Toscano, CS Mendes, J Bouvier, N Zampieri Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection Cell Rep, 2020;31(10):107741. 2020-01-01 [PMID: 32521266] (IHC, Mouse) IHC Mouse
DJ Flores, T Duong, LO Brandenber, A Mitra, A Shirali, JC Johnson, D Springer, A Noguchi, ZX Yu, SN Ebert, A Ludwig, BC Knollmann, MD Levin, K Pfeifer Conditional ablation and conditional rescue models for Casq2 elucidate the role of development and of cell type specific expression of Casq2 in the CPVT2 phenotype Hum. Mol. Genet., 2018;0(0):. 2018-01-01 [PMID: 29452352] (IHC-Fr, Mouse) IHC-Fr Mouse
N Hamada, I Iwamoto, H Tabata, KI Nagata MUNC18-1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development Acta Neuropathol Commun, 2017;5(1):92. 2017-01-01 [PMID: 29191246] (ICC, Primate) ICC Primate
K Onishi, A Uyeda, M Shida, T Hirayama, T Yagi, N Yamamoto, N Sugo Genome Stability by DNA polymerase ? in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development J. Neurosci., 2017;0(0):. 2017-01-01 [PMID: 28765330] (IHC, Mouse) IHC Mouse
S Bhattachar, M Bhakta, NV Munshi Phenotypically silent Cre recombination within the postnatal ventricular conduction system PLoS ONE, 2017;12(3):e0174517. 2017-01-01 [PMID: 28358866] (Western Blot, Mouse) Western Blot Mouse
NOVA2-mediated RNA regulation is required for axonal pathfinding during development Elife, 2016;5(0):. 2016-01-01 [PMID: 27223325] (IHC, Mouse) IHC Mouse
Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease. EMBO Mol Med, 2015;7(2):175-89. 2015-01-01 [PMID: 25592972] (Western Blot, Mouse) Western Blot Mouse
Show All 12 Publications.

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Gene Symbol CNTN2