N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

Images

 
Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - Paraformaldehyde-fixed HeLa, using GALNS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled ...read more
Immunohistochemistry-Paraffin: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GALNS. The exact sequence is proprietary.
Localization
Lysosome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GALNS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-32899 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

  • Chondroitinase
  • Chondroitinsulfatase
  • EC 3.1.6
  • EC 3.1.6.4
  • FLJ17434
  • FLJ42844
  • FLJ98217
  • GA6S
  • galactosamine (N-acetyl)-6-sulfate sulfatase
  • Galactose-6-Sulfate Sulfatase
  • GalNAc6S Sulfatase
  • GALNAC6S
  • GALNS
  • GAS
  • MPS4A
  • NAcetylgalactosamine6Sulfatase
  • N-Acetylgalactosamine-6-Sulfatase
  • N-acetylgalactosamine-6-sulfate sulfatase

Background

This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody (NBP1-32899) (0)

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Reviews for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody (NBP1-32899) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: WB.

Reviews using NBP1-32899:
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WB
(2)
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Human
(2)
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Images Ratings Applications Species Date Details
 
reviewed by:
Yadilette Rivera-Colon
WB Human 07/08/2010
View

Summary

ApplicationWestern Blot
Sample TestedHuman Protein transfected in Tn5 cells
SpeciesHuman
Lot40079
CommentsI have used it just a couple of times and so far so good. It actually better than the same antibody from other companies.

Blocking

Blocking DetailsBlocking Buffer: 2% BSA in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Details

Detection NotesSpecific Bands: 59 kDa, Non-Specific Bands: 25 kDa

Comments

CommentsI have used it just a couple of times and so far so good. It actually better than the same antibody from other companies.
 
reviewed by:
Yadilette Rivera-Colon
WB Human 06/24/2010
View

Summary

ApplicationWestern Blot
Sample TestedTn5 Cell supernatant
SpeciesHuman
Lot40037
CommentsIt cross-reacts with some other stuff, but nothing major.

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 or 25 degrees Celsius

Details

Detection NotesSpecific Bands: 58-60 kDa, Non-Specific Bands: 26 kDa

Comments

CommentsIt cross-reacts with some other stuff, but nothing major.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody (NBP1-32899) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
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Yadilette Rivera-Colon
07/08/2010
Application: WB
Species: Human

 
Yadilette Rivera-Colon
06/24/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol GALNS
Entrez