N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract7.5% SDS-PAGE GALNS antibody dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - Paraformaldehyde-fixed HeLa, using GALNS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled Red.

Immunohistochemistry-Paraffin: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.

Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000.

Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-32899] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug C2C12 whole cell lysate/extract 7.5% SDS-PAGE . GALNS antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human GALNS. The exact sequence is proprietary.
• Localization: Lysosome
• Predicted Species: Porcine (91%), Bovine (91%), Canine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GALNS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (81%), Xenopus laevis (84%), Xenopus Tropicalis (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

• Chondroitinase
• Chondroitinsulfatase
• EC 3.1.6
• EC 3.1.6.4
• FLJ17434
• FLJ42844
• FLJ98217
• GA6S
• galactosamine (N-acetyl)-6-sulfate sulfatase
• Galactose-6-Sulfate Sulfatase
• GalNAc6S Sulfatase
• GALNAC6S
• GALNS
• GAS
• MPS4A
• NAcetylgalactosamine6Sulfatase
• N-Acetylgalactosamine-6-Sulfatase
• N-acetylgalactosamine-6-sulfate sulfatase

Background

This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Reviews for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody (NBP1-32899) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Yadilette Rivera-Colon WB Human 07/08/2010

Summary

• Application: Western Blot
• Sample Tested: Human Protein transfected in Tn5 cells
• Species: Human
• Lot: 40079

reviewed by: Yadilette Rivera-Colon WB Human 06/24/2010

Summary

• Application: Western Blot
• Sample Tested: Tn5 Cell supernatant
• Species: Human
• Lot: 40037

Bioinformatics

• Gene Symbol: GALNS
• Entrez:
  • 2588(Human)