Tenascin R Antibody (619) [Unconjugated]

Tenascin R was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western blot shows lysates of Mouse Brain and Rat Brain. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tenascin R at approximately 185-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clone: 619
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Tenascin R Antibody (619) [Unconjugated] Summary

• Immunogen: “rest L2” fraction from bovine brain
• Specificity: Detects mouse and rat Tenascin R in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tenascin R Antibody (619) [Unconjugated]

• Janusin
• MGC149328
• Restrictin
• tenascin R (restrictin, janusin)
• Tenascin R
• tenascin-R
• TNR
• TN-R

Background

Tenascin R is an extracellular matrix glycoprotein belonging to the Tenascin family of multidomain adhesion molecules. It is expressed exclusively in the central nervous system and affects neuronal cell migration and neurite extension. Mouse and rat Tenascin R share 97% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tenascin R Antibody (MAB1624)(13)

Publications using MAB1624

• Sinha, A;Kowalchuk, AM;Khatibi, N;Matthews, RT;MacDonald, JL; Astrocytic Regulation of aberrant perineuronal net formation in Mecp2 -null Neocortex bioRxiv : the preprint server for biology 2025-08-13 [PMID: 40832202] (Western Blot, Immunocytochemistry, Mouse)
• Sinha, A;Nickerson, G;Bouyain, S;Matthews, RT; Contactin-1 is a critical neuronal cell surface receptor for perineuronal net structure bioRxiv : the preprint server for biology 2024-11-05 [PMID: 39605332] (Immunocytochemistry, Mouse)
• Ogawa, Y;Lim, BC;George, S;Oses-Prieto, JA;Rasband, JM;Eshed-Eisenbach, Y;Hamdan, H;Nair, S;Boato, F;Peles, E;Burlingame, AL;Van Aelst, L;Rasband, MN; Antibody-directed extracellular proximity biotinylation reveals that Contactin-1 regulates axo-axonic innervation of axon initial segments Nature communications 2023-10-26 [PMID: 37884508] (ICC, Rat)
• José Javier Miguel-Hidalgo, Erik Hearn, Mohadetheh Moulana, Khunsa Saleem, Austin Clark, Maggie Holmes et al. Reduced length of nodes of Ranvier and altered proteoglycan immunoreactivity in prefrontal white matter in major depressive disorder and chronically stressed rats Sci Rep 9/29/2023 [PMID: 37775676]
• Sinha, A;Kawakami, J;Cole, KS;Ladutska, A;Nguyen, MY;Zalmai, MS;Holder, BL;Broerman, VM;Matthews, RT;Bouyain, S; Protein-protein interactions between tenascin-R and RPTP?/phosphacan are critical to maintain the architecture of perineuronal nets The Journal of biological chemistry 2023-06-23 [PMID: 37356715] (ICC, Mouse)
• Y Ogawa, BC Lim, S George, JA Oses-Priet, JM Rasband, Y Eshed-Eise, S Nair, F Boato, E Peles, AL Burlingame, LV Aelst, MN Rasband Antibody-directed extracellular proximity biotinylation reveals Contactin-1 regulates axo-axonic innervation of axon initial segments bioRxiv : the preprint server for biology, 2023-03-06;0(0):. 2023-03-06 [PMID: 36945454] (ICC, Rat)
• Stevens SR, Longley CM, Ogawa Y et al. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K(+) channels eLife 6/28/2021 [PMID: 34180393]
• Stevens SR, Longley CM, Ogawa Y et al. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K(+) channels eLife 2021-06-28 [PMID: 34180393]
• N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020-10-14;11(1):5171. 2020-10-14 [PMID: 33057002] (IHC, Mouse)
• NF Eskici, S Erdem-Ozda, D Dayangac-E The altered expression of perineuronal net elements during neural differentiation Cell. Mol. Biol. Lett., 2018-02-13;23(0):5. 2018-02-13 [PMID: 29456557] (ICC, Western Blot, Mouse, Rat)
• J Hou, W Wang, X Quan, W Liang, Z Li, D Chen, H Han Quantitative Visualization of Dynamic Tracer Transportation in the Extracellular Space of Deep Brain Regions Using Tracer-Based Magnetic Resonance Imaging Med. Sci. Monit., 2017-09-03;23(0):4260-4268. 2017-09-03 [PMID: 28866708] (Western Blot, Rat)
• K. A. Giamanco, R. T. Matthews Deconstructing the perineuronal net: Cellular contributions and molecular composition of the neuronal extracellular matrix Neuroscience 8/30/2012 [PMID: 22659016]
• Huan Liu, Kathleen M. Guthrie Neuronal replacement in the injured olfactory bulb Experimental Neurology 4/1/2011 [PMID: 21310147]

Review for Tenascin R Antibody (MAB1624) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 02/10/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23069754
• Species: Mouse

Bioinformatics

• Gene Symbol: TNR