Neuritin Antibody - BSA Free

Western Blot: Neuritin Antibody [NBP1-77282] - Analysis of neuritin in Daudi cell lysate with neuritin antibody at (A) 5 and (B) 10 ug/mL.

Immunocytochemistry/ Immunofluorescence: Neuritin Antibody - BSA Free [NBP1-77282] - Immunofluorescence of Neuritin in human brain tissue with Neuritin antibody at 20 ug/mL.Green: Neuritin Antibody Blue: DAPI staining

Immunohistochemistry: Neuritin Antibody - BSA Free [NBP1-77282] - Immunohistochemistry of Neuritin in mouse heart tissue with Neuritin antibody at 5 ug/mL.

Immunohistochemistry: Neuritin Antibody - BSA Free [NBP1-77282] - Immunohistochemistry of Neuritin in human brain tissue with Neuritin antibody at 2.5 ug/ml.

Immunocytochemistry/ Immunofluorescence: Neuritin Antibody - BSA Free [NBP1-77282] - Immunofluorescence of Neuritin in Mouse Heart cells with Neuritin antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Neuritin Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 20 amino acid synthetic peptide from near the center of human neuritin. The immunogen is located within amino acids 70 - 120 of Neuritin. Amino Acid Squence: CQEGAKDMWDKLRKESKNLN
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NRN1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 5-10 ug/ml
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Neuritin Peptide (NBP1-77282PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Neuritin Antibody - BSA Free

• dJ380B8.2
• neuritin 1
• Neuritin
• NRN1
• NRNMGC44811

Background

As the nervous system of a complex organism develops, it establishes functional networks through the growth and retraction of synaptic connections from growing axons and dendrites. This synaptic remodeling involves neuro-transmitter signaling, activation of neurotrophin receptors and alterations in gene expression. One such gene whose expression is increased by neural activity is neuritin, a GPI-anchored protein that is expressed in postmitotic differentiating neurons of the developing nervous system. Its expression is also induced by the neurotrophins BDNF and NT-3. Purified recombinant neuritin promotes neurite outgrowth and arborization in primary embryonic neuronal cultures, suggesting that neuritin may play a role as a downstream effector of activity-induced neurite outgrowth. More recent experiments have shown that neuritin is required for the androgen-induced axonal elongation in motor neurons and is upregulated following spinal cord injury, suggesting that neuritin may also play a role in survival and axonal regeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NRN1
• Uniprot:
  • NP_057672()