Nestin Antibody (4D11)

Images

 
Western Blot: Nestin Antibody (4D11) [NBP1-92717] - Analysis analysis of tissue and cell lysates with mouse mAB against nestin, NBP1-92717, dilution 1:500 in green: [1] protein standard, [2] embryonic E18 rat brain, [3] ...read more
Immunocytochemistry/ Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to nestin, NBP1-92717, dilution 1:500 in red, and ...read more
Immunohistochemistry: Nestin Antibody (4D11) [NBP1-92717] - Staining in mouse cortex microslice section (from postnatal day 3). Image from a verified customer review.
Immunocytochemistry/ Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Mixed cultures of neonatal rat neurons and glia stained with NBP1-92717 (red), chicken antibody to Vimentin (NB300-223, green) and DNA (DAPI ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
4D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Nestin Antibody (4D11) Summary

Immunogen
Recombinant construct, amino acids 317-630 of the human Nestin protein expressed in and purified from E. coli. [UniProt# P48681]
Marker
Neural Stem Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NES
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:500
Application Notes
This Nestin (4D11) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western blot, where a band can be seen at approximately 240 kDa. IHC reported in verified customer review. Use in FLOW reported in scientific literature (PMID: 31203166).
Theoretical MW
240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-92717 in the following applications:

Publications
Read Publications using
NBP1-92717 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% Glycerol
Preservative
5mM Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified

Alternate Names for Nestin Antibody (4D11)

  • FLJ21841
  • NES
  • Nestin

Background

Nestin (NES) is a class VI intermediate filament protein which is unable to self-assemble or form homodimers because of a short NH2-terminus and is essential for the development of brain, eye etc. as well as for processes such as survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. It facilitates the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and the trafficking/distribution of IF proteins or other factors to daughter cells during progenitor cell division. Nestin is expressed in dividing cells during early stages of development e.g. CNS, PNS, myogenic or other tissues etc, whereas with differentiation, it is downregulated followed by replacement with tissue-specific intermediate filament proteins, and therefore, is widely used as a neuronal stem cell marker. Under pathological conditions, nestin is expressed in repair processes in CNS, muscle, liver, and infarcted myocardium, and in tumors, increased nestin correlates with aggressive growth, metastasis, and poor prognosis. Furthermore, nestin is more specifically expressed in proliferating small-sized tumor vessels in glioblastoma and gastric, colorectal, and prostate cancers than are other tumor vessel markers, indicating nestin's use as a marker for newly synthesized tumor vessels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-141
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
AF3844
Species: Hu, Mu
Applications: IHC
NB300-213
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, KD
AF1759
Species: Hu, Mu
Applications: WB, Simple Western, IP, ChIP, ICC
NB120-16518
Species: Hu, Mu, Rt, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MAB2018
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
NB100-79802
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-79843
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, CyTOF-ready
NB110-58870
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, KD
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-80467
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-37476
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for Nestin Antibody (NBP1-92717)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow, ICC/IF, IF.


Filter By Application
Flow
(1)
ICC/IF
(4)
IF
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP1-92717 Applications Species
Erkilic N, Sanjurjo-Soriano C, Manes G et al. Generation of a human iPSC line, INMi004-A, with a point mutation in CRX associated with autosomal dominant Leber congenital amaurosis Stem Cell Res 2019 Jun 03 [PMID: 31247521] (IF, Human) IF Human
Erkilic N, Sanjurjo-Soriano C, Diakatou M et al. Generation of a human iPSC line, INMi003-A, with a missense mutation in CRX associated with autosomal dominant cone-rod dystrophy Stem Cell Res Jun 7 2019 [PMID: 31203166] (Flow, Human) Flow Human
Goulielmaki M, Assimomytis N, Rozanc J et al. Establishment of CSUASOi001-A, a non-integrated induced pluripotent stem cell line from urine-derived cells of a Chinese patient carrying RS1 gene mutation Stem Cell Res May 17 2019 [PMID: 31141763] (ICC/IF, Human) ICC/IF Human
Guo Y, Zeng Q, Liu S et al. Generation of an iPS cell line via a non-integrative method using urine-derived cells from a patient with USH2A-associated retinitis pigmentosa. Stem Cell Res Apr 6 2018 [PMID: 29660607] (ICC/IF, Human) ICC/IF Human
Gabriel E, Gopalakrishnan J. Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders. J Vis Exp. Apr 14 2017 [PMID: 28448044]
Gabriel E, Ramani A, Karow U et al. Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids Cell Stem Cell Jan 23 2017 [PMID: 28132835] (ICC/IF, Human) ICC/IF Human
Moncla BJ, Chappell CA, Debo BM, Meyn LA. The Effects of Hormones and Vaginal Microflora on the Glycome of the Female Genital Tract: Cervical-Vaginal Fluid PLoS ONE 2016 Jul 21 [PMID: 27437931] (ICC/IF, Human) ICC/IF Human
Hockfield S, McKay RD. Identification of major cell classes in the developing mammalian nervous system. J Neurosci. 1985 Dec. [PMID: 4078630]
Lendahl U, Zimmerman LB, McKay RD et al. CNS stem cells express a new class of intermediate filament protein. Cell. 1990 Feb. [PMID: 1689217]

Review for Nestin Antibody (NBP1-92717) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-92717:
Filter by Applications
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 Nestin NBP1-92717
Enlarge
reviewed by:
Anonymous
Mouse 07/27/2017
View

Summary

Sample TestedMouse cortex
SpeciesMouse
Lot6315

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nestin Antibody (NBP1-92717) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Nestin Products

Bioinformatics Tool for Nestin Antibody (NBP1-92717)

Discover related pathways, diseases and genes to Nestin Antibody (NBP1-92717). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Nestin Antibody (NBP1-92717)

Discover more about diseases related to Nestin Antibody (NBP1-92717).
 

Pathways for Nestin Antibody (NBP1-92717)

View related products by pathway.

PTMs for Nestin Antibody (NBP1-92717)

Learn more about PTMs related to Nestin Antibody (NBP1-92717).
 

Research Areas for Nestin Antibody (NBP1-92717)

Find related products by research area.

Blogs on Nestin.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
07/27/2017
Application:
Species: Mouse

Bioinformatics

Gene Symbol NES
Entrez
Uniprot