Western blot shows lysates of rat liver tissue and mouse pancreas tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse/Rat Nesfatin‑1/Nucleobindin‑2 Antigen Affinity-purified Polyclonal Antibody ...read more
Nesfatin‑1/Nucleobindin‑2 was detected in immersion fixed frozen sections of rat brain (cortex) using Sheep Anti-Mouse/Rat Nesfatin‑1/Nucleobindin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Nesfatin‑1/Nucleobindin‑2 at approximately 55 kDa (as indicated) using 20 µg/mL of Sheep ...read more
E. coli-derived recombinant rat Nesfatin-1/Nucleobindin-2 Pro26-Leu106 Accession # Q9JI85
Detects rat Nesfatin-1/Nucleobindin-2 in direct ELISAs and mouse and rat Nesfatin-1/Nucleobindin-2 in Western blots. In direct ELISAs, approximately 7% cross-reactivity with recombinant human Nesfatin-1/Nucleobindin-2 is observed.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Sterile PBS to a final concentration of 0.2 mg/mL.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Nesfatin-1/Nucleobindin-2 Antibody [Unconjugated]
DNA-binding protein NEFA
NEFAGastric cancer antigen Zg4
Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a presumably secreted peptide derived from the translation product of the NUCB2 gene. Nesfatin‑1 is associated with neurons involved in feeding (ARH and PVH), fluid intake (SON and PVH) and autonomic activity, in beta -cells of the pancreas, endocrine cells in the stomach, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature rat Nesfatin-1 is 82 amino acids (aa) in length. Although it is 10 kDa in MW, its presence in SDS-PAGE is difficult to detect in biological fluids. It represents the N-terminal cleavage product of a 420 aa precursor termed NEFA/Nucleobindin‑2/NUCB2. NUCB2 contains a signal sequence (aa 1-24), Nesfatin-1 (aa 25-106), a DNA-binding site (aa 171-223), and two EF-hand regions (aa 241-276 and 293-328). Full-length NUCB2 is 48-55 kDa in size, and may be present extracellularly. There is a potential for multiple cleavages that would generate Nesfatin-1, Nesfatin-2 (aa 109-187) and Nesfatin-3 (aa 190-420). A 25 kDa peptide that represents Nesfatin-1 and -2 has been reported. Rat Nesfatin-1 (aa 25-106) shares 97% and 84% aa identity with mouse and human Nesfatin-1, respectively.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Nesfatin-1/Nucleobindin-2 Antibody [Unconjugated] and receive a gift card or discount.