Nesfatin-1/Nucleobindin-2 Antibody [Alexa Fluor® 750]

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Nesfatin-1/Nucleobindin-2 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant rat Nesfatin-1/Nucleobindin-2
  Pro26-Leu106
  Accession # Q9JI85
• Specificity: Detects rat Nesfatin-1/Nucleobindin-2 in direct ELISAs and mouse and rat Nesfatin-1/Nucleobindin-2 in Western blots. In direct ELISAs, approximately 7% cross-reactivity with recombinant human Nesfatin-1/Nucleobindin-2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nesfatin-1/Nucleobindin-2 Antibody [Alexa Fluor® 750]

• DNA-binding protein NEFA
• NEFA
• NEFAGastric cancer antigen Zg4
• Nesfatin1
• Nesfatin-1
• NUCB2
• nucleobindin 2
• Nucleobindin-2
• nucleobinding 2

Background

Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a presumably secreted peptide derived from the translation product of the NUCB2 gene. Nesfatin‑1 is associated with neurons involved in feeding (ARH and PVH), fluid intake (SON and PVH) and autonomic activity, in beta -cells of the pancreas, endocrine cells in the stomach, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature rat Nesfatin-1 is 82 amino acids (aa) in length. Although it is 10 kDa in MW, its presence in SDS-PAGE is difficult to detect in biological fluids. It represents the N-terminal cleavage product of a 420 aa precursor termed NEFA/Nucleobindin‑2/NUCB2. NUCB2 contains a signal sequence (aa 1-24), Nesfatin-1 (aa 25-106), a DNA-binding site (aa 171-223), and two EF-hand regions (aa 241-276 and 293-328). Full-length NUCB2 is 48-55 kDa in size, and may be present extracellularly. There is a potential for multiple cleavages that would generate Nesfatin-1, Nesfatin-2 (aa 109-187) and Nesfatin-3 (aa 190-420). A 25 kDa peptide that represents Nesfatin-1 and -2 has been reported. Rat Nesfatin-1 (aa 25-106) shares 97% and 84% aa identity with mouse and human Nesfatin-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.