Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free

Western Blot: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PVRL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PVRL1.

Immunocytochemistry/Immunofluorescence: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PVRL1.

Immunohistochemistry: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PVRL1 mouse monoclonal antibody.

Immunohistochemistry: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PVRL1 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: OTI6F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human PVRL1(NP_002846) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NECTIN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free

• CD111 antigen
• CD111
• CLPED1ectodermal dysplasia 4 (Margarita Island type)
• ED4
• Herpes virus entry mediator C
• Herpesvirus entry mediator C
• Herpesvirus Ig-like receptor
• HIgRPVRR
• HVEC
• HVECpoliovirus receptor-like 1
• MGC142031
• Nectin1
• Nectin-1
• OFC7nectin 1
• poliovirus receptor-related 1 (herpesvirus entry mediator C)
• poliovirus receptor-related protein 1
• PRR
• PRR1
• PRR1MGC16207
• PVRL1
• PVRR1
• PVRR1nectin-1
• SK-12

Background

The PVRL1 gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobu

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NECTIN1