Nectin-1/PVRL1 Antibody (OTI6F8)

Images

 
Western Blot: PVRL1 Antibody (6F8) [NBP2-01198] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PVRL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: PVRL1 Antibody (6F8) [NBP2-01198] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PVRL1.
Immunohistochemistry-Paraffin: PVRL1 Antibody (6F8) [NBP2-01198] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PVRL1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PVRL1 Antibody (6F8) [NBP2-01198] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PVRL1 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI6F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.54 mg/ml

Nectin-1/PVRL1 Antibody (OTI6F8) Summary

Immunogen
Full length human recombinant protein of human PVRL1(NP_002846) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NECTIN1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.54 mg/ml
Purity
Affinity purified

Alternate Names for Nectin-1/PVRL1 Antibody (OTI6F8)

  • CD111 antigen
  • CD111
  • CLPED1ectodermal dysplasia 4 (Margarita Island type)
  • ED4
  • Herpes virus entry mediator C
  • Herpesvirus entry mediator C
  • Herpesvirus Ig-like receptor
  • HIgRPVRR
  • HVEC
  • HVECpoliovirus receptor-like 1
  • MGC142031
  • Nectin1
  • Nectin-1
  • OFC7nectin 1
  • poliovirus receptor-related 1 (herpesvirus entry mediator C)
  • poliovirus receptor-related protein 1
  • PRR
  • PRR1
  • PRR1MGC16207
  • PVRL1
  • PVRR1
  • PVRR1nectin-1
  • SK-12

Background

This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nectin-1/PVRL1 Antibody (NBP2-01198) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-72929AF350
Alexa Fluor 405 NBP2-72929AF405
Alexa Fluor 488 NBP2-72929AF488
Alexa Fluor 532 NBP2-72929AF532
Alexa Fluor 594 NBP2-72929AF594
Alexa Fluor 647 NBP2-72929AF647
Alexa Fluor 700 NBP2-72929AF700
Alexa Fluor 750 NBP2-72929AF750
Biotin NBP2-72929B
DyLight 350 NBP2-72929UV
DyLight 405 NBP2-72929V
DyLight 488 NBP2-72929G
DyLight 550 NBP2-72929R
DyLight 594 NBP2-72929DL594
DyLight 650 NBP2-72929C
DyLight 680 NBP2-72929FR
DyLight 755 NBP2-72929IR
FITC NBP2-72929F
HRP NBP2-72929H
Janelia Fluor 549 NBP2-72929JF549
Janelia Fluor 646 NBP2-72929JF646

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Bioinformatics Tool for Nectin-1/PVRL1 Antibody (NBP2-01198)

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Diseases for Nectin-1/PVRL1 Antibody (NBP2-01198)

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Bioinformatics

Gene Symbol NECTIN1
Entrez