HVEM/TNFRSF14 Antibody (94801) [Unconjugated]

Images

 
Human peripheral blood lymphocytes were stained with Mouse Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB356, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ELISA(Cap)
Clone
94801
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

HVEM/TNFRSF14 Antibody (94801) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human HVEM/TNFRSF14
Pro37-Val202 (Ser108Thr and Ala140Arg)
Accession # AAB58354
Specificity
Detects human HVEM/TNFRSF14 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CD30 and no cross-reactivity with rh4-1BB, rhCD27, rhCD40, rhDR3, rhDR6, rhFas, rhGITR, rhNGF-R, rhOPG, rhRANK, recombinant mouse (rm) RANK, rhTAJ, rhTNF RI, rhTNF RII, or rmTNF RI is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF14
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human HVEM/TNFRSF14 Biotinylated Antibody (Catalog number BAF356)
Standard: Recombinant Human HVEM/TNFRSF14 Fc Chimera (Catalog number 356-HV)
Publications
Read Publications using
MAB356 in the following applications:

  • 2 publications
  • 2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HVEM/TNFRSF14 Antibody (94801) [Unconjugated]

  • ATAR
  • CD270 antigen
  • CD270
  • CD40-like protein
  • Herpes virus entry mediator A
  • Herpesvirus entry mediator A
  • HveA
  • HVEM
  • HVEMTR2HVEAATAR
  • LIGHTR
  • TNFRSF14
  • tumor necrosis factor receptor superfamily member 14
  • tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator)
  • Tumor necrosis factor receptor-like 2
  • tumor necrosis factor receptor-like gene2

Background

Herpesvirus entry mediator (HVEM), also referred to as TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. In the TNF superfamily nomenclature, HVEM is referred to as TNFRSF14. Human HVEM cDNA encodes a 283 amino acid (aa) protein with a probable 36 aa signal peptide, a 166 aa extracellular domain, a 23 aa transmembrane region and a 58 aa cytoplasmic region. The extracellular domain of HVEM contains several cysteine-rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor-associated factor) interaction domain. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D. Two TNF superfamily ligands, including the secreted TNF‑ beta  (lymphotoxin  alpha ) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LT beta R, the receptor for lymphotoxin alpha beta heterotrimer.

  1. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
  2. Mauri, D.N. et al. (1998) Immunity 8:21.
  3. Montgomery, R.I. et al. (1996) Cell 87:427.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HVEM/TNFRSF14 Antibody (MAB356)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow, Neut.


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Flow
(2)
Neut
(2)
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Bioinformatics

Gene Symbol TNFRSF14
Entrez
Uniprot