Renin R Antibody

Western Blot: Renin R Antibody [NB100-1318] - (0.5ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry: Renin R Antibody [NB100-1318] - Immunofluorescence staining of LV anterior wall at day 3 after gene transfer showing Renin R predominantly in cardiomyocytes.A) DAPI (blue),B) prolyl 4-hydroxylase staining for fibroblasts (red),C) Renin R (green) andD, Merge. Representative image is shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22911790/) licensed under a CC-BY license.

Flow Cytometry: Renin R Antibody [NB100-1318] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: Renin R Antibody [NB100-1318] - (0.5ug/ml) staining of Human Cerebellum (A) and Heart (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

(P)RR is located into the cardiac myocytes in the rat heart.Immunofluorescence staining of LV anterior wall at day 3 after gene transfer showing (P)RR predominantly in cardiomyocytes. A, DAPI (blue), B, prolyl 4-hydroxylase beta staining for fibroblasts (red), C, (P)RR (green) and D, Merge. Representative image is shown.

Immunocytochemistry/ Immunofluorescence: Renin R Antibody [NB100-1318] - (P)RR is located into the cardiac myocytes in the rat heart.Immunofluorescence staining of LV anterior wall at day 3 after gene transfer showing (P)RR predominantly in cardiomyocytes. A, DAPI (blue), B, prolyl 4-hydroxylase beta staining for fibroblasts (red), C, (P)RR (green) & D, Merge. Representative image is shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22911790), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Renin R Antibody Summary

• Immunogen: Peptide with sequence C-SIIYRMTNQKIRMD corresponding to C-Terminus according to NP_005756.2.
• Localization: Potential integral membrane protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ATP6AP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1.0 ug/ml
• Application Notes: Western blot: Approx 38-39kDa band observed in Human Cerebellum and in Human and Rat Heart lysates (calculated MW of 39.0kDa according to Human NP_005756.2 and Rat NP_001007092.1). Primary incubation 1 hour at room temperature.
  Use in ICC/IF reported in scientific literature (PMID: 34228176).
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Kidney Whole Tissue Lysate (Adult Whole Normal)
  Renin R Overexpression Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22911790).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Renin R Antibody

• APT6M8-9
• ATP6AP2
• ATP6IP2
• ATP6M8-9
• ATP6M8-9MRXE
• ATPase H(+)-transporting lysosomal accessory protein 2
• ATPase H(+)-transporting lysosomal-interacting protein 2
• ATPase, H+ transporting, lysosomal accessory protein 2
• ATPase, H+ transporting, lysosomal interacting protein 2
• CAPER
• ELDF10
• Embryonic liver differentiation factor 10
• ER-localized type I transmembrane adaptor
• H+ transporting, lysosomal (vacuolar proton pump) membrane sectorassociated protein M8-9
• HT028
• M8-9
• MGC99577
• MRXE
• MSTP009
• N14F
• Renin R
• renin receptor
• ReninR
• vacuolar proton ATP synthase membrane sector associated protein M8-9
• V-ATPase M8.9 subunit
• XMRE

Background

This gene encodes a protein which is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Renin R Antibody (NB100-1318)(9)

Publications using NB100-1318

• Figueiredo M, Daryadel A, Sihn G Et Al. The (pro)renin receptor (ATP6ap2) facilitates receptor-mediated endocytosis and lysosomal function in the renal proximal tubule Pflugers Archiv : European journal of physiology 2021-08-01 [PMID: 34228176] (ICC/IF)
• Daryadel A, Bourgeois S, Figueiredo MF et al. Colocalization of the (Pro)renin Receptor/Atp6ap2 with H+-ATPases in Mouse Kidney but Prorenin Does Not Acutely Regulate Intercalated Cell H+-ATPase Activity. PLoS One 2016-01-29 [PMID: 26824839] (WB)
• Mahmud H, Candido WM, van Genne L et al. Cardiac function and architecture are maintained in a model of cardiorestricted overexpression of the prorenin-Renin receptor. PLoS ONE 2014-03-03 [PMID: 24587131] (WB, Mouse)
• Moilanen AM, Rysa J, Serpi R et al. (Pro)renin Receptor Triggers Distinct Angiotensin II-Independent Extracellular Matrix Remodeling and Deterioration of Cardiac Function plos ONE 2012-01-01 [PMID: 22911790] (IF/IHC, Rat)
• Mahmud H, Sillje HH, Cannon MV et al. Regulation of the (pro)renin-renin receptor in cardiac remodelling. J Cell Mol Med. 2012-04-01 [PMID: 21722305]
• Valenzuela R, Barroso-Chinea P, Villar-Cheda B et al. Location of Prorenin Receptors in Primate Substantia Nigra: Effects on Dopaminergic Cell Death. J Neuropathol Exp Neurol. 2010-10-11 [PMID: 20940627]
• Pringle KG, Zakar T, Yates D et al. Molecular evidence of a (pro)renin/ (pro)renin receptor system in human intrauterine tissues in pregnancy and its association with PGHS-2. J Renin Angiotensin Aldosterone Syst 2010-08-11 [PMID: 20702505] (IHC-P, Human)
• Shan Z, Cuadra AE, Sumners C, Raizada MK. Characterization of a functional (pro)renin receptor in rat brain neurons. Exp Physiol;93(5):701-8. 2008-05-01 [PMID: 18326551]
• Ludwig J, Kerscher S, Brandt U et al. Identification and characterization of a novel 9.2-kDa membrane sector-associated protein of vacuolar proton-ATPase from chromaffin granules. J Biol Chem 1998-05-01 [PMID: 9556572]

Bioinformatics

• Gene Symbol: ATP6AP2
• Entrez:
  • 10159(Human)
  • 70495(Mouse)
• Uniprot:
  • O75787()