Dectin-1/CLEC7A Antibody (259931)

Images

 
Dectin‑1/CLEC7A was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human Dectin‑1/CLEC7A Monoclonal Antibody (Catalog # MAB1859) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready, ICC/IF
Clone
259931
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Dectin-1/CLEC7A Antibody (259931) Summary

Additional Information
Clone 259931 was used by HLDA to establish CD designation
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A
Thr66-Met201
Accession # NP_072092
Specificity
Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CLEC7A
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
MAB1859 in the following applications:

Publications
Read Publications using
MAB1859 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dectin-1/CLEC7A Antibody (259931)

  • Beta-glucan receptor
  • BGR
  • CD369
  • CLEC7A
  • CLECSF12
  • CLECSF12DC-associated C-type lectin 1
  • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12
  • C-type lectin domain family 7 member A
  • C-type lectin domain family 7, member A
  • C-type lectin superfamily member 12
  • Dectin1
  • Dectin-1
  • DECTIN1CANDF4
  • Dendritic cell-associated C-type lectin 1
  • dendritic cell-associated C-type lectin-1
  • hDectin-1
  • lectin-like receptor 1

Background

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dectin-1/CLEC7A Antibody (MAB1859)(21)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: Blocking, Flow Cytometry, ICC, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Blocking
(1)
Flow Cytometry
(3)
ICC
(2)
Immunoprecipitation
(1)
Neutralization
(13)
Western Blot
(1)
All Applications
Filter By Species
Human
(21)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using MAB1859 Applications Species
JC de Quaglia, AM Della Cole, TP Gardizani, GG Romagnoli, R Kaneno, LA Dias-Melic Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis J Immunol Res, 2019;2019(0):1529189. 2019 [PMID: 30882002] (Neutralization, Human) Neutralization Human
B Kan, C Michalski, H Fu, HHT Au, K Lee, EA Marchant, MF Cheng, E Anderson-B, M Aharoni-Si, P Tilley, RG Mirmira, CJ Ross, DS Luciani, E Jan, PM Lavoie Cellular metabolism constrains innate immune responses in early human ontogeny Nat Commun, 2018;9(1):4822. 2018 [PMID: 30446641] (Neutralization, Human) Neutralization Human
MMM Kaisar, M Ritter, C Del Fresno, HS Jónasdótti, AJ van der Ha, LR Pelgrom, G Schramm, LE Layland, D Sancho, C Prazeres d, M Giera, M Yazdanbakh, B Everts Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses PLoS Biol., 2018;16(4):e2005504. 2018 [PMID: 29668708] (Neutralization, Human) Neutralization Human
M Swidergall, NV Solis, MS Lionakis, SG Filler EphA2 is an epithelial cell pattern recognition receptor for fungal ?-glucans Nat Microbiol, 2017;0(0):. 2017 [PMID: 29133884] (Western Blot, Human) Western Blot Human
CC Yeh, HC Horng, H Chou, HY Tai, HD Shen, SL Hsieh, PH Wang Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells Int J Mol Sci, 2017;18(3):. 2017 [PMID: 28335387] (Neutralization, Human) Neutralization Human
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro PLoS ONE, 2016;11(11):e0167410. 2016 [PMID: 27898740] (Neutralization, Human) Neutralization Human
Gazendam R, van Hamme J, Tool A, Hoogenboezem M, van den Berg J, Prins J, Vitkov L, van de Veerdonk F, van den Berg T, Roos D, Kuijpers T Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects. J Immunol, 2016;196(3):1272-83. 2016 [PMID: 26718340] (Neutralization, Human) Neutralization Human
Elder M, Webster S, Williams D, Gaston J, Goodall J TSLP production by dendritic cells is modulated by IL-1beta and components of the endoplasmic reticulum stress response. Eur J Immunol, 2015;46(2):455-63. 2015 [PMID: 26573878] (Neutralization, Human) Neutralization Human
Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li X, Hori T, Zurawski S, Liu Y, Zurawski G, Oh S Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization. J Immunol, 2015;195(4):1723-31. 2015 [PMID: 26123355] (Neutralization, Human) Neutralization Human
Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production. PLoS ONE, 2015;10(3):e0120948. 2015 [PMID: 25793979] (Blocking, Human) Blocking Human
Show All 21 Publications.

Reviews for Dectin-1/CLEC7A Antibody (MAB1859) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using MAB1859:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Other 02/12/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23514738
SpeciesOther

Blocking

Blocking DetailsSee PMID 23514738

Details

Detection NotesSee PMID 23514738

Comments

CommentsIndependent review from antibodyresource.com
 
reviewed by:
Anonymous
IF Human 02/12/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24424029
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24424029

Details

Detection NotesSee PMID 24424029

Comments

CommentsIndependent review from antibodyresource.com

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FAQs for Dectin-1/CLEC7A Antibody (MAB1859) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
1
4
1
3
0
2
0
1
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Anonymous
02/12/2015
Application: IF
Species: Other

 
Anonymous
02/12/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol CLEC7A
Uniprot