DARC Antibody

Images

 
Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - Staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.
Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - DARC (3ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DARC Antibody Summary

Immunogen
Peptide with sequence HRAELSPSTENSSQLDFED-C corresponding to N-Terminus according to NP_001116423.1, NP_002027.2.
Specificity
This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DARC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.3 ug/ml
  • Flow Cytometry 1:1000
  • Immunohistochemistry 3 ug/ml
  • Immunohistochemistry-Paraffin 3 ug/ml
  • Peptide ELISA detection limit 1:16000
Application Notes
WB: Approx 37kDa band observed in Human Kidney and Liver lysates (calculated MW of 35.6kDa according to NP_002027.2).
IHC-P: In paraffin embedded human kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Human Lung showing endothelial staining.
Flow cytometry: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Liver Lysate (NB820-59232)
Publications
Read Publications using
NB100-2421 in the following applications:

  • IHC
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DARC Antibody

  • ACKR1
  • CCBP1
  • CD234 antigen
  • CD234
  • DARC
  • Dfy
  • Duffy Antigen
  • Duffy antigen/chemokine receptor
  • Duffy blood group antigen
  • Duffy blood group, chemokine receptor
  • Fy Antigen
  • Fy glycoprotein
  • FYDuffy blood group
  • Glycoprotein D
  • GPD
  • GPDWBCQ1
  • GpFy
  • Plasmodium vivax receptor

Background

Gene ID(s): 2532 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DARC Antibody (NB100-2421)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DARC
Entrez
Uniprot