NDC80 Antibody (9G3.23)

Images

 
Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, ...read more
Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] diluted at 1:500. Red: alpha ...read more
Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.
Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse ...read more
Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Mouse anti-Hec1 and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned ...read more
Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using HEC1(NB100-338) antibody (Green) at 1:500 ...read more
Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in fixed human cell lines of RPE1, H4, and 42MGBA. Image courtesy of anonymous customer product review.
Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in PTK1 cells.Verified customer review from 1DegreeBio.

Product Details

Summary
Reactivity Hu, Mu, HaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IP, PLA
Clone
9G3.23
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NDC80 Antibody (9G3.23) Summary

Immunogen
Human HEC1 protein consisting of amino acids 56-642.
Localization
Nuclear
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NDC80
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1-5 ug/ml
  • Proximity Ligation Assay
  • Western Blot 1:500-1:3000
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 5 Reviews rated 4.6
using
NB100-338 in the following applications:

Publications
Read Publications using
NB100-338 in the following applications:

  • 36 publications
  • WB
    4 publications

Reactivity Notes

Kangaroo rat (100%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for NDC80 Antibody (9G3.23)

  • HEC1KNTC2
  • HECRetinoblastoma-associated protein HEC
  • Highly expressed in cancer protein
  • highly expressed in cancer, rich in leucine heptad repeats (yeast)
  • highly expressed in cancer, rich in leucine heptad repeats
  • hsHec1
  • hsNDC80
  • kinetochore associated 2
  • Kinetochore protein Hec1
  • kinetochore protein NDC80 homolog
  • Kinetochore-associated protein 2
  • NDC80 homolog, kinetochore complex component (S. cerevisiae)
  • TID3

Background

Human HEC1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results in the impairment of chromosome congression and results in spindle checkpoint activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NDC80 Antibody (NB100-338)(48)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(36)
WB
(4)
All Applications
Filter By Species
Human
(13)
All Species
Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using NB100-338 Applications Species
Roy B, Han SJY, Fontan AN et al. Aurora B phosphorylates Bub1 to promote spindle assembly checkpoint signaling Current biology : CB Nov 24 2021 [PMID: 34861183]
Neahring L, Cho NH, Dumont S. Opposing motors provide mechanical and functional robustness in the human spindle Developmental cell Sep 30 2021 [PMID: 34614397]
Uchida KSK, Jo M, Nagasaka K et al. Kinetochore stretching-mediated rapid silencing of the spindle-assembly checkpoint required for failsafe chromosome segregation Current biology : CB Feb 22 2021 [PMID: 33651990] (ICC/IF, Human) ICC/IF Human
Wimbish RT, DeLuca KF, Mick JE et al. The Hec1/Ndc80 tail domain is required for force generation at kinetochores, but is dispensable for kinetochore-microtubule attachment formation and Ska complex recruitment Mol. Biol. Cell May 13 2020 [PMID: 32401635]
Raab M, Sanhaji M, Matthess Y et al. PLK1 has tumor-suppressive potential in APC-truncated colon cancer cells. Nat Commun. Mar 16 2018 [PMID: 29549256] (ICC/IF, Human) ICC/IF Human
DeLuca KF, Meppelink A, Broad AJ et al. Aurora A kinase phosphorylates Hec1 to regulate metaphase kinetochore-microtubule dynamics J. Cell Biol. [PMID: 29187526] (ICC/IF) ICC/IF
Dumitru AMG, Rusin SF, Clark AEM et al. Cyclin A/Cdk1 modulates Plk1 activity in prometaphase to regulate kinetochore-microtubule attachment stability Elife. 2017 Nov 20 [PMID: 29154753] (ICC/IF, Human)

Details:
HEC1 IF antibody used at a dilution of 1:500
ICC/IF Human
Niikura Y, Kitagawa R, Ogi H, Kitagawa K. SGT1-HSP90 complex is required for CENP-A deposition at centromeres Cell Cycle 2017 Aug 17 [PMID: 28816574] (Human) Human
Tauchman EC, Boehm FJ, DeLuca JG. Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells. Nat Commun. 2015 Dec 01 [PMID: 26620470] (WB, ICC/IF, Human) WB, ICC/IF Human
Kleyman M, Kabeche L, Compton DA. STAG2 promotes error correction in mitosis by regulating kinetochore-microtubule attachments. J. Cell. Sci. 2014 Jul 29 [PMID: 25074805] (ICC/IF, Human)

Details:
Outer kinetochore component Hec1/NDC80 antibody used in ICC-IF on HCT116 and RPE1 cells for measuring centromere stretch (Fig. 1D, supplementary material Fig. S2A)
ICC/IF Human
Show All 48 Publications.

Reviews for NDC80 Antibody (NB100-338) (5) 4.65

Average Rating: 4.6
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Human, Other.

Reviews using NB100-338:
Filter by Applications
Immunofluorescence
(4)
Western Blot
(1)
All Applications
Filter by Species
Human
(4)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence NDC80 NB100-338
Enlarge
5
reviewed by:
Lilian Kabeche
Immunofluorescence Human 04/21/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedRPE1 cells
SpeciesHuman
Western Blot NDC80 NB100-338
Enlarge
4
reviewed by:
Verified Customer
Western Blot Human 11/06/2013
View

Summary

ApplicationWestern Blot
Sample TestedU2OS whole cell lysate
SpeciesHuman
Immunofluorescence NDC80 NB100-338
Enlarge
4
reviewed by:
Verified Customer
Immunofluorescence Other 11/06/2013
View

Summary

ApplicationImmunofluorescence
Sample TestedPTK1 cells
SpeciesOther
  5
reviewed by:
Duane Compton
Immunofluorescence Human 10/04/2013
View

Summary

ApplicationImmunofluorescence
SpeciesHuman
Immunofluorescence NDC80 NB100-338
Enlarge
5
reviewed by:
Verified Customer
Immunofluorescence Human 06/06/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedFixed cell lines (RPE1, H4, 42MGBA)
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NDC80 Antibody (NB100-338) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.6
5
3
4
2
3
0
2
0
1
0

Lilian Kabeche
04/21/2016
Application: Immunofluorescence
Species: Human

Verified Customer
11/06/2013
Application: Western Blot
Species: Human

Verified Customer
11/06/2013
Application: Immunofluorescence
Species: Other

Bioinformatics

Gene Symbol NDC80
Entrez
Uniprot