NCOA2 Antibody

Western Blot: NCOA2 Antibody [NB100-388] - Detection of Mouse NCOA2/ SRC2 by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 antibody NB100-388 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: NCOA2 Antibody [NB100-388] - Detection of human NCOA2/ SRC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa(15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 antibody NB100-388 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRC2 was also immunoprecipitated by a previous lot (lot 1) and by rabbit anti-NCOA1/SRC2 antibody NBP2-22162, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

NCOA2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 700 and 750 of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499).
• Specificity: Antibody was affinity purified using an epitope specific to SRC2 immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCOA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 1-4 ug/mg lysate
  • Western Blot 1:1000-1:10000
• Application Notes: NB 100-388 may be used for Western Blot, where a band at ~160 kDa is seen, representing SRC2 protein. Though this antibody will recognize cytosolic protein in Western blot, it does much better with nuclear extracts. It may also be used for immunoprecipitation on HeLa nuclear extracts. Suggested working dilutions: * Western Blot - 1:1,000-1:10,000 Immunocytochemistry - Not Determined Immunoprecipitation - 1-4 ug/mg *The investigator should determine the optimal working dilution for a specific application. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28552353).
• Control:

  NCOA2 Overexpression Lysate
  HeLa Nuclear Cell Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28552353).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NCOA2 Antibody

• bHLHe75
• bHLHe75MGC138808
• Class E basic helix-loop-helix protein 75
• EC 2.3.1.48
• GRIP1glucocorticoid receptor-interacting protein-1
• HTIF2
• KAT13C
• NCOA2
• NCoA-2SRC2
• nuclear receptor coactivator 2
• TIF2
• TIF2hTIF2
• Transcriptional intermediary factor 2

Background

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-2 (SRC2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCOA2 Antibody (NB100-388)(1)

Publication using NB100-388

• Long AF, Udy DB, Dumont S. Hec1 Tail Phosphorylation Differentially Regulates Mammalian Kinetochore Coupling to Polymerizing and Depolymerizing Microtubules Curr. Biol. 2017-06-05 [PMID: 28552353] (ICC/IF, Rat)

Review for NCOA2 Antibody (NB100-388) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Kalpana Acharya WB Mouse 10/10/2016

Summary

• Application: Western Blot
• Sample Tested: Brain (hypothalamus) tissue
• Species: Mouse
• Lot: A1

Comments

• Comments: western blot was done on proteins from adult female mouse hypothalamus to probe for SRC-2 fraction that was associated with Progestin receptors.

Bioinformatics

• Gene Symbol: NCOA2
• Entrez:
  • 10499(Human)
• Uniprot:
  • Q15596(Human)