NCOA2 Antibody

Images

 
Western Blot: NCOA2 Antibody [NB100-388] - Detection of Mouse NCOA2/ SRC2 by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 ...read more
Independent Antibodies: Immunoprecipitation: NCOA2 Antibody [NB100-388] - Detection of human NCOA2/ SRC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa(15 and 50 ug for WB; 1 mg for ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

NCOA2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 700 and 750 of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499).
Specificity
Antibody was affinity purified using an epitope specific to SRC2 immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCOA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
NB 100-388 may be used for Western Blot, where a band at ~160 kDa is seen, representing SRC2 protein. Though this antibody will recognize cytosolic protein in Western blot, it does much better with nuclear extracts. It may also be used for immunoprecipitation on HeLa nuclear extracts. Suggested working dilutions: * Western Blot - 1:1,000-1:10,000 Immunocytochemistry - Not Determined Immunoprecipitation - 1-4 ug/mg *The investigator should determine the optimal working dilution for a specific application. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28552353).
Control
NCOA2 Overexpression Lysate
HeLa Nuclear Cell Lysate
NCOA2 Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-388 in the following applications:

Publications
Read Publication using
NB100-388 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28552353).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NCOA2 Antibody

  • bHLHe75
  • bHLHe75MGC138808
  • Class E basic helix-loop-helix protein 75
  • EC 2.3.1.48
  • GRIP1glucocorticoid receptor-interacting protein-1
  • HTIF2
  • KAT13C
  • NCOA2
  • NCoA-2SRC2
  • nuclear receptor coactivator 2
  • TIF2
  • TIF2hTIF2
  • Transcriptional intermediary factor 2

Background

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-2 (SRC2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-310
Species: Hu, Mu, Rt, Pm
Applications: WB, ChIP, IP
NB100-314
Species: Hu
Applications: WB, IP
NB100-56603
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IHC-P
NB100-381
Species: Hu
Applications: WB, ChIP, IP
NB100-61050
Species: Hu
Applications: WB, IHC, IHC-P, IP, PLA
NB200-331
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP2-37537
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB300-731
Species: Hu, Mu, Rt, Gp, Rb, Sh, Ye
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, B/N
NB100-616
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, Flow, ICC/IF, IP, In vitro, Flow-IC
NBP1-76729
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
MAB5285
Species: Hu
Applications: WB, Simple Western
NBP3-09392
Species: Hu, Mu
Applications: WB
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NBP1-89146
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-38530
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-81909
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-16684
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-388
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IP

Publications for NCOA2 Antibody (NB100-388)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
All Applications
Filter By Species
Rat
(1)
All Species

Review for NCOA2 Antibody (NB100-388) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-388:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot NCOA2 NB100-388
Enlarge
4
reviewed by:
Anonymous
WB Mouse 10/10/2016
View

Summary

ApplicationWestern Blot
Sample TestedBrain (hypothalamus) tissue
SpeciesMouse
LotA1

Comments

Commentswestern blot was done on proteins from adult female mouse hypothalamus to probe for SRC-2 fraction that was associated with Progestin receptors.

Product General Protocols

View specific protocols for NCOA2 Antibody (NB100-388): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NCOA2 Antibody (NB100-388) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional NCOA2 Products

Bioinformatics Tool for NCOA2 Antibody (NB100-388)

Discover related pathways, diseases and genes to NCOA2 Antibody (NB100-388). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NCOA2 Antibody (NB100-388)

Discover more about diseases related to NCOA2 Antibody (NB100-388).
 

Pathways for NCOA2 Antibody (NB100-388)

View related products by pathway.

PTMs for NCOA2 Antibody (NB100-388)

Learn more about PTMs related to NCOA2 Antibody (NB100-388).
 

Research Areas for NCOA2 Antibody (NB100-388)

Find related products by research area.

Blogs on NCOA2

There are no specific blogs for NCOA2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
10/10/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol NCOA2
Entrez
Uniprot