This Nanog antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin embedded tissue. In ICC/IF, nuclear staining was observed in DGCR8 knockout mouse embryonic stem cells. In IHC-P, staining was observed in the nucleus mouse testes. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for Nanog Antibody
homeobox protein NANOG
Homeobox transcription factor Nanog
homeobox transcription factor Nanog-delta 48
In stem cell development, the influence of the transcription factor Nanog is important for appropriate differentiation, or conversely to maintain their pluripotent state. Nanog is important for stem cell growth, maintenance and differentiation, but is usually co-expressed and co-activated by other factors. Islet survivor cells, generated for progenitor cell testing, have nuclear expression of OCT4, SOX2 and Nanog, indicating a probability that they can maintain pluripotent characteristics in experimentation. In brain cancer, there are some stem cell like behaviors, and the cancer cells exhibiting these characteristics demonstrate high concentrations of Nanog, influenced by p53.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Nanog Antibody (NBP2-13177). (Showing 1 - 1 of 1 FAQ).
Can NBP2-13177 be used to do nuclear staining?
Yes, Homeobox protein NANOG is localized in nucleus and our Nanog antibody is expected to result in nuclear staining. Most of the normal tissues show moderate nuclear staining in fractions of cells, however, strong nuclear staining was observed in airway epithelium and brain tissues. The gastrointestinal tract, kidney, pancreas, seminiferous duct cells, and cells in female genital tract as well as lymphoid tissues are generally weakly stained or negative. Nanog is not expressed in oocytes and spermatogonia and in many somatic organs, ovary, testis, fibroblast and hematopoietic cell lines. Testicular cancerous tissues, however, show a very strong expression whereas a significantly high expression can also be found in thyoid, ovary and endometrial cancer tissues.
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