Nanog Antibody

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Immunohistochemistry-Paraffin: Nanog Antibody [NBP2-13177] - IHC staining of Nanog in mouse testis using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P, WB (-)
Clonality
Polyclonal
Host
Rabbit
Concentration
1.0 mg/ml

Order Details

Nanog Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region of the mouse Nanog protein (within residues 1-50). [Swiss-Prot Q80Z64 ]
Localization
Nucleus.
Marker
Embryonic Stem Cell Marker
Clonality
Polyclonal
Host
Rabbit
Gene
NANOG
Purity
Immunogen affinity purified
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This Nanog antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin embedded tissue. In ICC/IF, nuclear staining was observed in DGCR8 knockout mouse embryonic stem cells. In IHC-P, staining was observed in the nucleus mouse testes. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Mouse

Alternate Names for Nanog Antibody

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
  • Nanog

Background

In stem cell development, the influence of the transcription factor Nanog is important for appropriate differentiation, or conversely to maintain their pluripotent state. Nanog is important for stem cell growth, maintenance and differentiation, but is usually co-expressed and co-activated by other factors. Islet survivor cells, generated for progenitor cell testing, have nuclear expression of OCT4, SOX2 and Nanog, indicating a probability that they can maintain pluripotent characteristics in experimentation. In brain cancer, there are some stem cell like behaviors, and the cancer cells exhibiting these characteristics demonstrate high concentrations of Nanog, influenced by p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ICC/IF, IHC, IHC-P, WB (-)

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for Nanog Antibody (NBP2-13177). (Showing 1 - 1 of 1 FAQ).

  1. Can NBP2-13177 be used to do nuclear staining?
    • Yes, Homeobox protein NANOG is localized in nucleus and our Nanog antibody is expected to result in nuclear staining. Most of the normal tissues show moderate nuclear staining in fractions of cells, however, strong nuclear staining was observed in airway epithelium and brain tissues. The gastrointestinal tract, kidney, pancreas, seminiferous duct cells, and cells in female genital tract as well as lymphoid tissues are generally weakly stained or negative. Nanog is not expressed in oocytes and spermatogonia and in many somatic organs, ovary, testis, fibroblast and hematopoietic cell lines. Testicular cancerous tissues, however, show a very strong expression whereas a significantly high expression can also be found in thyoid, ovary and endometrial cancer tissues.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Nanog.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol NANOG
Entrez
Uniprot

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