DGCR8 knockout Mouse embryonic stem cells

Images

 
Immunocytochemistry/ Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with ...read more
Flow Cytometry: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - DCGR8 mouse embryonic stem cells were cultured for 5 passages in StemXVivo Mouse Pluripotent Stem Cell Media (R&D Systems, Catalog # CCM025). At ...read more
Immunocytochemistry/ Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Nanog antibody (NB100-58842) was tested in with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with ...read more
In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing directly on a gelatinized tissue culture flask.
In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing on a Mitomycin C MEF cell feeder layer.
In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 -/- ES cell colonies growing on a gamma-irradiated MEF cell feeder layer.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications In vitro

Order Details

DGCR8 knockout Mouse embryonic stem cells Summary

Specificity
DGCR8 knockout Mouse embryonic stem cells
Gene
DGCR8

Applications/Dilutions

Application Notes
Recommended Media: StemXVivo Mouse Pluripotent Stem Cell Media Kit (R&D Systems, Cat# CCM025). Please see Protocol for aditional culturing conditions. In vivo assay was reported in scientific literature.
Publications
Read Publications using
NBA1-19349 in the following applications:

Packaging, Storage & Formulations

Storage
Store in gas phase of liquid nitrogen.
Buffer
Cells are supplied in 2 ml quantities (about 1x10 ^ 6 cells/ml) in Freezing Media (60% DMEM, 20% FBS, 20% DMSO)

Notes

Shipping is on dry ice. Proper long-term strorage is gas phase of liquid nitrogen. This cell line was constructed in the v6.5 ES cell line (Catolog No. NBP1-41162).

Alternate Names for DGCR8 knockout Mouse embryonic stem cells

  • C22orf12
  • DGCRK6chromosome 22 open reading frame 12
  • DiGeorge syndrome critical region 8
  • DiGeorge syndrome critical region gene 8
  • Gy1
  • microprocessor complex subunit DGCR8
  • pasha

Background

MicroRNAs are abundant, 21-25 nucleotide non-coding RNAs that are important endogenous regulators of gene expression. MicroRNAs work by specifically regulating messenger RNAs (mRNAs), and are predicted to regulate hundreds of genes individually and simultaneously, affecting cellular functions such as differentiation, development, proliferation, and apoptosis. The specific regulation at both the transcription and the translation level opens the possibility to use microRNAs as targets for the development of drugs and for the diagnosis of several human diseases. Novus Biologicals now offers a novel a line of DGCR8 knockout mouse embryonic stem (ES) cells to study the global role of microRNAs. Embryonic stem cells provide a tool for the study of the molecular mechanisms of early mammalian development. The DGCR8 cells have been genetically altered at the locus of the dgcr8 gene such that no functional DGCR8 protein can be produced, resulting in the global, but specific, loss of micro RNAs. The DGCR8 knockout mouse embryonic stem (ES) cells are unique because they allow for the specific study of the global role of microRNAs. Unlike the Dicer cell line, the only other comparable line for studying microRNAs, the DGCR8 cell line appears to be specific for microRNAs, whereas Dicer also affects other classes of small RNAs. Mouse Dicer1 knockout ES cells have been useful for inferring the role for small RNAs in ES cell differentiation. Dicer is required for the maturation of at least two classes of small RNAs: microRNAs and short interfering RNAs (siRNAs). Thus, in studies using Dicer1 knockout ES cells it can be difficult to isolate whether the lack of microRNAs alone are the cause of the observed phenotype. DGCR8 is part of the microprocessor complex, which is composed of the proteins DGCR8 and Drosha. The microprocessor complex handles microRNA processing; unlike Dicer, this complex seems to be specific to microRNAs. Of the two components of the microprocessor complex, Drosha has been reported to have a role in ribosomal RNA processing, possibly in a distinct protein complex, while DGCR8 does not. Therefore, DGCR8 may be the only member of the processing pathway that is specific to microRNAs. These cells are useful for the specific study of microRNA function in both embryonic stem cells as well as derivatives of embryonic stem cells (e.g. differentiated cells from embryonic stem cells).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Publications for DGCR8 knockout Mouse embryonic stem cells (NBA1-19349)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: In vitro, WB.


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In vitro
(3)
WB
(1)
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Mouse
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBA1-19349 Applications Species
Macias S, Cordiner RA, Gautier P et al. DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs. Mol Cell 2015 Dec 17 [PMID: 26687677] (WB) WB
Krawczynski K, Najmula J, Bauersachs S, Kaczmarek MM. MicroRNAome of porcine conceptuses and trophoblasts: expression profile of micrornas and their potential to regulate genes crucial for establishment of pregnancy. Biol Reprod. [PMID: 25472924]
Ciaudo C, Jay F, Okamoto I et al. RNAi-Dependent and Independent Control of LINE1 Accumulation and Mobility in Mouse Embryonic Stem Cells. PLoS Genet. 2013 Nov [PMID: 24244175]
Jiang K, Ren C, Nair VD. MicroRNA-137 represses Klf4 and Tbx3 during differentiation of mouse embryonic stem cells. Stem Cell Res. 2013 Sep 13 [PMID: 24084696]
Heras SR, Macias S, Plass M et al. The Microprocessor controls the activity of mammalian retrotransposons. Nat Struct Mol Biol. 2013 Sep 1 [PMID: 23995758]
Stankiewicz TR, Schroeder EK, Kelsey NA et al. C-terminal binding proteins are essential pro-survival factors that undergo caspase-dependent downregulation during neuronal apoptosis. Mol Cell Neurosci 2013 Jul 13 [PMID: 23859824]
Macias S, Plass M, Stajuda A et al. DGCR8 HITS-CLIP reveals novel functions for the Microprocessor Nat Struct Mol Biol 2012 Aug [PMID: 22796965]
Havens MA, Reich AA, Duelli DM, Hastings ML. Biogenesis of mammalian microRNAs by a non-canonical processing pathway. Nucleic Acids Research. 2012 Jan 23. [PMID: 22270084]
Wang et al. DGCR8 is essential for microRNA biogenesis silencing of embryonic stem cell self-renewal. Nat Genet; 39(3):380-5. 2007 Mar. [PMID: 17259983] (In vitro) In vitro
Lei Z, van Mil A, van de Vrugt AM. Dgcr8 is Indispensable for Cardiac Lineage Specification in Embryonic Stem Cells Stem CellResearch & Therapy. 2015 Jan 15 (In vitro)

Details:
DGCR8 knockout Mouse embryonic stem cells and v6.5 Mouse embryonic stem cells used for experiments involving in vito cardiac differentiation. Dgcr8 KO mouse embryonic stem cells alongwith v6.5 WT controls were also characterized for the loss of Dgcr8 protein (in the Dgcr8 KO-ESCs) with WB, Genotyping, ICC-IF and RNA profiling (Figure 1).
In vitro
Show All 11 Publications.

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Product General Protocols

View specific protocols for DGCR8 knockout Mouse embryonic stem cells (NBA1-19349): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DGCR8 knockout Mouse embryonic stem cells (NBA1-19349). (Showing 1 - 1 of 1 FAQs).

  1. Hello, I am currently working with the DGCR8 knockout mouse embryonic stem cell line and would like to know how you generated the knockout? i.e. which exon is spliced out, this will aid me with primer design.
    • This publication describes the generation of the knockout. Please let me know if you need more information than is provided in this PMC Article.

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Bioinformatics

Gene Symbol DGCR8