Immunocytochemistry/ Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with ...read more
Flow Cytometry: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - DCGR8 mouse embryonic stem cells were cultured for 5 passages in StemXVivo Mouse Pluripotent Stem Cell Media (R&D Systems, Catalog # CCM025). At ...read more
Immunocytochemistry/ Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Nanog antibody (NB100-58842) was tested in with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with ...read more
In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing directly on a gelatinized tissue culture flask.
In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing on a Mitomycin C MEF cell feeder layer.
In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 -/- ES cell colonies growing on a gamma-irradiated MEF cell feeder layer.
Recommended Media: StemXVivo Mouse Pluripotent Stem Cell Media Kit (R&D Systems, Cat# CCM025). Please see Protocol for aditional culturing conditions. In vivo assay was reported in scientific literature.
Read Publications using NBA1-19349 in the following applications:
Cells are supplied in 2 ml quantities (about 1x10 ^ 6 cells/ml) in Freezing Media (60% DMEM, 20% FBS, 20% DMSO)
Shipping is on dry ice. Proper long-term strorage is gas phase of liquid nitrogen. This cell line was constructed in the v6.5 ES cell line (Catolog No. NBP1-41162).
Alternate Names for DGCR8 knockout Mouse embryonic stem cells
DGCRK6chromosome 22 open reading frame 12
DiGeorge syndrome critical region 8
DiGeorge syndrome critical region gene 8
microprocessor complex subunit DGCR8
MicroRNAs are abundant, 21-25 nucleotide non-coding RNAs that are important endogenous regulators of gene expression. MicroRNAs work by specifically regulating messenger RNAs (mRNAs), and are predicted to regulate hundreds of genes individually and simultaneously, affecting cellular functions such as differentiation, development, proliferation, and apoptosis. The specific regulation at both the transcription and the translation level opens the possibility to use microRNAs as targets for the development of drugs and for the diagnosis of several human diseases. Novus Biologicals now offers a novel a line of DGCR8 knockout mouse embryonic stem (ES) cells to study the global role of microRNAs. Embryonic stem cells provide a tool for the study of the molecular mechanisms of early mammalian development. The DGCR8 cells have been genetically altered at the locus of the dgcr8 gene such that no functional DGCR8 protein can be produced, resulting in the global, but specific, loss of micro RNAs. The DGCR8 knockout mouse embryonic stem (ES) cells are unique because they allow for the specific study of the global role of microRNAs. Unlike the Dicer cell line, the only other comparable line for studying microRNAs, the DGCR8 cell line appears to be specific for microRNAs, whereas Dicer also affects other classes of small RNAs. Mouse Dicer1 knockout ES cells have been useful for inferring the role for small RNAs in ES cell differentiation. Dicer is required for the maturation of at least two classes of small RNAs: microRNAs and short interfering RNAs (siRNAs). Thus, in studies using Dicer1 knockout ES cells it can be difficult to isolate whether the lack of microRNAs alone are the cause of the observed phenotype. DGCR8 is part of the microprocessor complex, which is composed of the proteins DGCR8 and Drosha. The microprocessor complex handles microRNA processing; unlike Dicer, this complex seems to be specific to microRNAs. Of the two components of the microprocessor complex, Drosha has been reported to have a role in ribosomal RNA processing, possibly in a distinct protein complex, while DGCR8 does not. Therefore, DGCR8 may be the only member of the processing pathway that is specific to microRNAs. These cells are useful for the specific study of microRNA function in both embryonic stem cells as well as derivatives of embryonic stem cells (e.g. differentiated cells from embryonic stem cells).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Lei Z, van Mil A, van de Vrugt AM. Dgcr8 is Indispensable for Cardiac Lineage Specification in Embryonic Stem Cells Stem CellResearch & Therapy. 2015 Jan 15 (In vitro)
Details: DGCR8 knockout Mouse embryonic stem cells and v6.5 Mouse embryonic stem cells used for experiments involving in vito cardiac differentiation. Dgcr8 KO mouse embryonic stem cells alongwith v6.5 WT controls were also characterized for the loss of Dgcr8 protein (in the Dgcr8 KO-ESCs) with WB, Genotyping, ICC-IF and RNA profiling (Figure 1).
FAQs for DGCR8 knockout Mouse embryonic stem cells (NBA1-19349). (Showing 1 - 1 of 1 FAQs).
Hello, I am currently working with the DGCR8 knockout mouse embryonic stem cell line and would like to know how you generated the knockout? i.e. which exon is spliced out, this will aid me with primer design.
This publication describes the generation of the knockout. Please let me know if you need more information than is provided in this PMC Article.