NAC1 Antibody (3)

Product Discontinued

NAC1 Antibody (3) Summary

• Immunogen: Recombinant human Nac1 protein. [UniProt# Q96RE7]
• Localization: Nuclear
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NACC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation Assay dependent
  • Western Blot 0.3 ug/ml
• Application Notes: This Nac1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin,Immunoprecipitation and Flow Cytometry. In WB a band is seen at ~57 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Nuclear Cell Lysate

Reactivity Notes

Human and rat.Mouse reactivity reported in scientific literature (PMID: 23922682)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for NAC1 Antibody (3)

• BEND8
• BTB (POZ) domain containing 14B
• BTB/POZ domain-containing protein 14B
• BTBD14B
• BTBD30
• NAC1
• NAC1BEN domain containing 8
• NAC-1FLJ37383
• NACC1
• nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
• nucleus accumbens-associated protein 1
• transcriptional repressor NAC1

Background

The BTB/POZ gene family shares a conserved BTB/POZ protein-protein interaction motif at the N terminus that mediates homodimer or heterodimer formation. NAC1, a member of this family, has been found to be overexpressed in ovarian cancer. This protein appears to play a critical role in tumorigenesis and in the growth and survival of tumor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NAC1 Antibody (NB110-77345)(15)

Publications using NB110-77345

• Zhang Y, Dong S, Wang X et al. Mechanistic Insights of NAC1 Nuclear Export and Its Role in Ovarian Cancer Resistance to Docetaxel Research Square 2022-11-30 [PMID: 37019189] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human)
• Xia Z, Xu G, Nie L et al. NAC1 Potentiates Cellular Antiviral Signaling by Bridging MAVS and TBK1 J. Immunol. 2019-06-24 [PMID: 31235549]
• Saneyoshi T, Matsuno H, Suzuki A et al. Identification of a small-molecule compound that inhibits homodimerization of oncogenic NAC1 protein and sensitizes cancer cells to anticancer agents J. Biol. Chem. 2019-05-17 [PMID: 31101655] (IHC-P, IP, WB, Mouse)
• Ren YJ, Wang XH, Ji C et al. Silencing of NAC1 Expression Induces Cancer Cells Oxidative Stress in Hypoxia and Potentiates the Therapeutic Activity of Elesclomol Front Pharmacol. 2017-11-07 [PMID: 29163184] (WB, Human)
• Nakayama K, Rahman M, Rahman MT. Nucleus accumbens-1/GADD45GIP1 axis mediates cisplatin resistance through cellular senescence in ovarian cancer. ONCOLOGY LETTERS. 2017-01-04 [PMID: 28599472] (IF/IHC, Human)
• Zhang Y, Ren YJ, Guo LC et al. Nucleus accumbens-associated protein-1 promotes glycolysis and survival of hypoxic tumor cells via the HDAC4-HIF-1a axis. Oncogene. 2017-03-20 [PMID: 28319066] (WB, Mouse)
• Korutla L, Furlong HA 4th, Mackler SA. NAC1, A POZ/BTB protein interacts with Parkin and may contribute to Parkinson's disease. Neuroscience. 2013-11-11 [PMID: 24231739] (WB, ICC/IF)
• Yap KL, Sysa-Shah P, Bolon B et al. Loss of NAC1 Expression Is Associated with Defective Bony Patterning in the Murine Vertebral Axis. PLoS One 2013-07-26 [PMID: 23922682] (IHC-P, Mouse)
• Scofield M, Korutla L, Jackson TG et al. NAC1, a POZ/BTB protein binds to TDP-43 and has a potential role in Amyotrophic Lateral Sclerosis Neuroscience 2012-09-25 [PMID: 23022214] (ICC/IF, Rat)
• Rahman MT, Nakayama K, Ishikawa M et al. NAC1, a BTB/POZ Protein Overexpressed in Uterine Sarcomas Anticancer Res 2012-09-01 [PMID: 22993327]
• Rahman MT, Nakayama K, Rahman M et al. Fatty acid synthase expression associated with NAC1 is a potential therapeutic target in ovarian clear cell carcinomas. Br J Cancer. 2012-05-31 [PMID: 22653145] (WB, Human)
• Constantin D, McCullough J, Mahajan R, Greenhaff PL. Novel events in the molecular regulation of muscle mass in critically-ill patients. J Physiol. 2011-06-13 [PMID: 21669975] (WB, Human)
• Wu PH, Hung SH, Ren T et al. Cell cycle-dependent alteration in NAC1 nuclear body dynamics and morphology Phys Biol 2011-02-01 [PMID: 21301057]
• Shih IeM, Nakayama K, Wu G, Nakayama N, Zhang J, Wang TL. Amplification of the ch19p132 .NACC1 locus in ovarian high-grade serous carcinoma. Mod Pathol;24(5):638-45. 2011-05-01 [PMID: 21240255] (IF/IHC, Human)
• Nakayama, K et al. A BTB/POZ protein, NAC-1, is related to tumor recurrence and is essential for tumor growth and survival. Proc Natl Acad Sci U S A;103(49):18739-44. 2006-12-05 [PMID: 17130457] (IHC-P, ICC/IF, Human)

Bioinformatics

• Gene Symbol: NACC1