Profilin 1 Antibody (OTI1D5)

Images

 
Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)
Immunocytochemistry/ Immunofluorescence: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1.
Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.
Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Profilin 1 antibody, and then ...read more
Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Western Blot: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Profilin 1 monoclonal antibody.
Western Blot: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: ...read more
Immunohistochemistry-Paraffin: Profilin 1 Antibody (1D5) [NBP2-02577] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.
Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Profilin 1 Antibody (1D5) [NBP2-02577] - Analysis of Jurkat cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI1D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Profilin 1 Antibody (OTI1D5) Summary

Immunogen
Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PFN1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
14.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-02577 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for Profilin 1 Antibody (OTI1D5)

  • PFN1
  • PROF1
  • Profilin 1
  • Profilin I
  • profilin-1

Background

The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Profilin 1 Antibody (NBP2-02577)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Profilin 1 Antibody (NBP2-02577) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73605AF350
Alexa Fluor 405 NBP2-73605AF405
Alexa Fluor 488 NBP2-73605AF488
Alexa Fluor 532 NBP2-73605AF532
Alexa Fluor 594 NBP2-73605AF594
Alexa Fluor 647 NBP2-73605AF647
Alexa Fluor 700 NBP2-73605AF700
Alexa Fluor 750 NBP2-73605AF750
Allophycocyanin NBP2-73605APC
Biotin NBP2-73605B
DyLight 350 NBP2-73605UV
DyLight 405 NBP2-73605V
DyLight 488 NBP2-73605G
DyLight 550 NBP2-73605R
DyLight 594 NBP2-73605DL594
DyLight 650 NBP2-73605C
DyLight 680 NBP2-73605FR
DyLight 755 NBP2-73605IR
FITC NBP2-73605F
HRP NBP2-73605H
Janelia Fluor 549 NBP2-73605JF549
Janelia Fluor 646 NBP2-73605JF646
PE NBP2-73605PE
PerCP NBP2-73605PCP

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Bioinformatics Tool for Profilin 1 Antibody (NBP2-02577)

Discover related pathways, diseases and genes to Profilin 1 Antibody (NBP2-02577). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Profilin 1.

Profiling the Profilin 1 Antibody
Profilin-1, or Pfn-1, is a small actin-binding protein which plays an essential role controlling the growth of microfilaments. Profilin 1 and Profilin 2 have similar biochemical properties but are expressed in different tissues. The Profilin 1 antibod...  Read full blog post.

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Bioinformatics

Gene Symbol PFN1
Entrez