NAC1 Antibody (3)

Images

 
Western Blot: Nac1 Antibody (3) [NB110-77345] - Detection of NAC-1 in HeLa nuclear extracts.
Immunocytochemistry/ Immunofluorescence: Nac1 Antibody (3) [NB110-77345] - Staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).
Immunohistochemistry-Paraffin: Nac1 Antibody (3) [NB110-77345] - Nuclear staining of paraffin-embedded ovarian cancer cells
Flow Cytometry: Nac1 Antibody (3) [NB110-77345] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using Nac1 antibody (dark blue). Isotype control shown in orange. An antibody concentration ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NAC1 Antibody (3) Summary

Immunogen
Recombinant human Nac1 protein. [UniProt# Q96RE7]
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NACC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 ug/ml
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation Assay dependent
Application Notes
This Nac1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin,Immunoprecipitation and Flow Cytometry. In WB a band is seen at ~57 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC9)
Publications
Read Publications using
NB110-77345 in the following applications:

  • 3 publications
  • IHC
    2 publications
  • 2 publications
  • WB
    4 publications

Reactivity Notes

Human and rat.Mouse reactivity reported in scientific literature (PMID: 23922682)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NAC1 Antibody (3)

  • BEND8
  • BTB (POZ) domain containing 14B
  • BTB/POZ domain-containing protein 14B
  • BTBD14B
  • BTBD30
  • NAC1
  • NAC1BEN domain containing 8
  • NAC-1FLJ37383
  • NACC1
  • nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
  • nucleus accumbens-associated protein 1
  • transcriptional repressor NAC1

Background

The BTB/POZ gene family shares a conserved BTB/POZ protein-protein interaction motif at the N terminus that mediates homodimer or heterodimer formation. NAC1, a member of this family, has been found to be overexpressed in ovarian cancer. This protein appears to play a critical role in tumorigenesis and in the growth and survival of tumor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

Publications for NAC1 Antibody (NB110-77345)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IHC
(2)
IHC-P
(2)
WB
(4)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB110-77345 Applications Species
Nakayama K, Rahman M, Rahman MT. Nucleus accumbens-1/GADD45GIP1 axis mediates cisplatin resistance through cellular senescence in ovarian cancer. ONCOLOGY LETTERS. Jan 4 2017 [PMID: 28599472] (IHC, Human) IHC Human
Zhang Y, Ren YJ, Guo LC et al. Nucleus accumbens-associated protein-1 promotes glycolysis and survival of hypoxic tumor cells via the HDAC4-HIF-1a axis. Oncogene. Mar 20 2017 [PMID: 28319066] (WB, Mouse) WB Mouse
Korutla L, Furlong HA 4th, Mackler SA. NAC1, A POZ/BTB protein interacts with Parkin and may contribute to Parkinson's disease. Neuroscience. 2013 Nov 11 [PMID: 24231739] (WB, ICC/IF) WB, ICC/IF
Yap KL, Sysa-Shah P, Bolon B et al. Loss of NAC1 Expression Is Associated with Defective Bony Patterning in the Murine Vertebral Axis. PLoS One 2013 Jul 26 [PMID: 23922682] (IHC-P, Mouse) IHC-P Mouse
Scofield M, Korutla L, Jackson TG et al. NAC1, a POZ/BTB protein binds to TDP-43 and has a potential role in Amyotrophic Lateral Sclerosis Neuroscience 2012 Sep 25 [PMID: 23022214] (ICC/IF, Rat) ICC/IF Rat
Rahman MT, Nakayama K, Ishikawa M et al. NAC1, a BTB/POZ Protein Overexpressed in Uterine Sarcomas Anticancer Res 2012 Sep [PMID: 22993327]
Rahman MT, Nakayama K, Rahman M et al. Fatty acid synthase expression associated with NAC1 is a potential therapeutic target in ovarian clear cell carcinomas. Br J Cancer. 2012 May 31. [PMID: 22653145] (WB, Human) WB Human
Constantin D, McCullough J, Mahajan R, Greenhaff PL. Novel events in the molecular regulation of muscle mass in critically-ill patients. J Physiol. 2011 Jun 13. [PMID: 21669975] (WB, Human) WB Human
Wu PH, Hung SH, Ren T et al. Cell cycle-dependent alteration in NAC1 nuclear body dynamics and morphology Phys Biol 2011 Feb [PMID: 21301057]
Shih IeM, Nakayama K, Wu G, Nakayama N, Zhang J, Wang TL. Amplification of the ch19p132.NACC1 locus in ovarian high-grade serous carcinoma. Mod Pathol;24(5):638-45. 2011 May. [PMID: 21240255] (IHC, Human) IHC Human
Show All 11 Publications.

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Product General Protocols

View specific protocols for NAC1 Antibody (NB110-77345): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NAC1 Antibody (NB110-77345) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-77345AF405
Alexa Fluor 488 Labeled NB110-77345AF488
Alexa Fluor 647 Labeled NB110-77345AF647
Alexa Fluor 700 Labeled NB110-77345AF700
Allophycocyanin Labeled NB110-77345APC
Biotin Labeled NB110-77345B
DyLight 350 Labeled NB110-77345UV
DyLight 405 Labeled NB110-77345V
DyLight 488 Labeled NB110-77345G
DyLight 550 Labeled NB110-77345R
DyLight 650 Labeled NB110-77345C
DyLight 680 Labeled NB110-77345FR
DyLight 755 Labeled NB110-77345IR
FITC Labeled NB110-77345F
HRP Labeled NB110-77345H
PE Labeled NB110-77345PE

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NAC1 NB110-77345

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Blogs on NAC1

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Bioinformatics

Gene Symbol NACC1
Entrez