PLZF Antibody [Unconjugated]

Images

 
Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was ...read more
Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for PLZF at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLZF ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

PLZF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PLZF
Met1-Gln254
Accession # Q05516
Specificity
Detects human PLZF in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ZBTB16
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
Publications
Read Publications using
AF2944 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLZF Antibody [Unconjugated]

  • PLZF
  • PLZFzinc finger and BTB domain-containing protein 16
  • ZBTB16
  • zinc finger and BTB domain containing 16
  • zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia)
  • Zinc finger protein 145
  • Zinc finger protein PLZF
  • ZNF145
  • ZNF145Promyelocytic leukemia zinc finger protein

Background

Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PLZF Antibody (AF2944)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC, IHC - paraffin, IHC Paraffin-embedded, IP, WB.


Filter By Application
ICC
(1)
IHC - paraffin
(1)
IHC Paraffin-embedded
(1)
IP
(1)
WB
(3)
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Human
(3)
Mouse
(4)
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Showing Publications 1 - 7 of 7.
Publications using AF2944 Applications Species
The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells Nat. Immunol, 2016;0(0):. 2016 [PMID: 27992400] (WB,, Mouse) WB Mouse
Ai-Ping Mao Multiple layers of transcriptional regulation by PLZF in NKT-cell development Proc Natl Acad Sci USA, 2016;0(0):. 2016 [PMID: 27325774] (IP,, Human) IP Human
Li M, Huang R, Jiang X, Chen Y, Zhang Z, Zhang X, Liang P, Zhan S, Cao S, Songyang Z, Huang J CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. PLoS ONE, 2015;10(11):e0143148. 2015 [PMID: 26599493] (IHC - paraffin,, Mouse) IHC - paraffin Mouse
Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D PAX7 expression defines germline stem cells in the adult testis. J Clin Invest, 2014;124(9):3929-44. 2014 [PMID: 25133429] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Makela J, Toppari J, Rivero-Muller A, Ventela S Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture. PLoS ONE, 2014;9(3):e90088. 2014 [PMID: 24619130] (ICC,, Mouse) ICC Mouse
Cao, JingPing, Zhu, Shu, Zhou, Wei, Li, Jie, Liu, Chang, Xuan, HanQing, Yan, Jie, Zheng, Lin, Zhou, LiXin, Yu, JianXiu, Chen, GuoQiang, Huang, YiRan, Yu, Zhuo, Feng, LiXin PLZF mediates the PTEN/AKT/FOXO3a signaling in suppression of prostate tumorigenesis. PLoS ONE, 2013;8(12):e77922. 2013 [PMID: 24339862] (WB,, Human) WB Human
Mathew, Rebecca, Seiler, Michael, Scanlon, Seth T, Mao, Ai-ping, Constantinides, Michael, Bertozzi-Villa, Clara, Singer, Jeffrey, Bendelac, Albert BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs. Nature, 2012;491(7425):618-21. 2012 [PMID: 23086144] (WB,, Human) WB Human

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ZBTB16
Entrez
Uniprot