PLZF Antibody [Unconjugated]


Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was more
Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for PLZF at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLZF more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western

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PLZF Antibody [Unconjugated] Summary

E. coli-derived recombinant human PLZF
Accession # Q05516
Detects human PLZF in direct ELISAs and Western blots.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF2944 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLZF Antibody [Unconjugated]

  • PLZF
  • PLZFzinc finger and BTB domain-containing protein 16
  • ZBTB16
  • zinc finger and BTB domain containing 16
  • zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia)
  • Zinc finger protein 145
  • Zinc finger protein PLZF
  • ZNF145
  • ZNF145Promyelocytic leukemia zinc finger protein


Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PLZF Antibody (AF2944)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-F, IHC-P, Immunoprecipitation, Western Blot.

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Flow Cytometry
Western Blot
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF2944 Applications Species
V Barroca, C Racine, L Pays, P Fouchet, M Coureuil, I Allemand The netrin-1 receptor UNC5C contributes to the homeostasis of undifferentiated spermatogonia in adult mice Stem Cell Research, 2022;60(0):102723. 2022 [PMID: 35247845] (Flow Cytometry, Human) Flow Cytometry Human
S Yamanaka, Y Horiuchi, S Matsuoka, K Kido, K Nishino, M Maeno, N Shibata, H Kosako, T Sawasaki A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues Nature Communications, 2022;13(1):183. 2022 [PMID: 35013300] (Western Blot, Human) Western Blot Human
WL Lei, YY Li, TG Meng, Y Ning, SM Sun, CH Zhang, Y Gui, ZB Wang, WP Qian, QY Sun Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis Cell Death & Disease, 2021;12(10):883. 2021 [PMID: 34580275] (IHC, Mouse) IHC Mouse
MM Mikedis, Y Fan, PK Nicholls, T Endo, EK Jackson, SA Cobb, DG de Rooij, DC Page DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors Elife, 2020;9(0):. 2020 [PMID: 32686646] (IHC-P, Mouse) IHC-P Mouse
K Fu, S Tian, H Tan, C Wang, H Wang, M Wang, Y Wang, Z Chen, Y Wang, Q Yue, Q Xu, S Zhang, H Li, J Xie, M Lin, M Luo, F Chen, L Ye, K Zheng Biological and RNA regulatory function of MOV10 in mammalian germ cells BMC Biol., 2019;17(1):39. 2019 [PMID: 31088452] (IHC-F, Mouse) IHC-F Mouse
RL Tang, LQ Fan PLZFposc-KITpos-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation Asian J. Androl., 2019;21(3):309-318. 2019 [PMID: 30719983] (IHC, Mouse) IHC Mouse
SP Jamin, FG Petit, C Kervarrec, F Smagulova, D Illner, H Scherthan, M Primig EXOSC10/Rrp6 is post-translationally regulated in male germ cells and controls the onset of spermatogenesis Sci Rep, 2017;7(1):15065. 2017 [PMID: 29118343] (IHC-P, Mouse) IHC-P Mouse
K Gassei, Y Sheng, A Fayomi, P Mital, M Sukhwani, CC Lin, KA Peters, A Althouse, H Valli, KE Orwig DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis Biol. Reprod., 2017;96(3):707-719. 2017 [PMID: 28339678] (IHC-P, Rat) IHC-P Rat
The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells Nat. Immunol, 2016;0(0):. 2016 [PMID: 27992400] (Western Blot, Mouse) Western Blot Mouse
Ai-Ping Mao Multiple layers of transcriptional regulation by PLZF in NKT-cell development Proc Natl Acad Sci USA, 2016;0(0):. 2016 [PMID: 27325774] (Immunoprecipitation, Human) Immunoprecipitation Human
Show All 15 Publications.

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Gene Symbol ZBTB16