CART/CARTPT Antibody (113612)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
113612
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

CART/CARTPT Antibody (113612) Summary

Immunogen
E. coli-derived recombinant human CART
Ala37-Leu116
Accession # Q16568
Specificity
Detects human CART aa 28‑116 in direct ELISAs and Western blots. Also recognizes a truncated recombinant human CART containing amino acids 69‑116.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CARTPT
Purity
Protein A or G purified from ascites
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB163 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from ascites
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CART/CARTPT Antibody (113612)

  • CART prepropeptide
  • CART
  • CARTcocaine and amphetamine regulated transcript
  • CARTPT
  • cocaine- and amphetamine-regulated transcript protein

Background

CART, also known as cocaine and amphetamine-related transcript, is a 10 kDa secreted polypeptide produced by neuronal cell types in a variety of locations.  In human, the molecule is synthesized as a 116 amino acid (aa) precursor with a 27 aa signal sequence an 89 aa mature segment.  There are six C-terminal cysteines that form three intrachain disulfide bonds, and the potential exists for both sulfation and phosphorylation in the N-terminal region.  There are numerous monobasic and dibasic sites for enzyme cleavage, and multiple, presumably proteolytically-generated short forms are known to exist.  These include a 5K, 48 aa peptide (CART 42‑89), and a 4.3K, 40 aa peptide (CART 49-89).  Human CART is 98% aa identical to both moyuse and rat CART (1-89).  Although CART suppresses feeding and increases pain tolerance, there would appear to be differences between the various forms of CART on other functions.   In addition, anatomical location is associated with differing lengths of CART.  For example, 89 aa and 79 aa forms are found in the adrenal, while 48 aa and 44 aa forms are found in the hypothalamus.

  1. Douglass, J. & S. Daoud (1996) Gene 169:241.
  2. Douglass, J. et al. (1995) J. Neurosci. 15:247.
  3. Adams, L.D. et al. (1999) Brain Res. 848:137.
  4. Thim, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:2722.
  5. Bannon, A.W. et al. (2001) J. Pharmacol. Exp. Ther. 299:1021.
  6. Kuhar, M.J. & L.L. Yoho (1999) Synapse 33:163.
  7. Thim, L. et al. (1998) FEBS Lett. 428:263.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CART/CARTPT Antibody (MAB163)(10)

We have publications tested in 5 confirmed species: Human, Rat, Guinea Pig, Porcine,  Human.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, Western Blot.


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ICC
(1)
IHC
(2)
IHC-Fr
(2)
Western Blot
(1)
All Applications
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Human
(1)
Rat
(2)
Guinea Pig
(1)
Porcine
(2)
 Human
(1)
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Showing Publications 1 - 10 of 10.
Publications using MAB163 Applications Species
Kobori, N;Moore, AN;Redell, JB;Dash, PK; Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function Journal of neuroinflammation 2023-07-04 [PMID: 37403174] (Western Blot, Human) Western Blot Human
Kobori, N;Moore, AN;Redell, JB;Dash, PK; Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function Journal of neuroinflammation 2023-07-04 [PMID: 37403174] ( Human)  Human
Arpan G. Mazumder, Amélie M. Julé, Paul F. Cullen, Daniel Sun Astrocyte heterogeneity within white matter tracts and a unique subpopulation of optic nerve head astrocytes iScience 2022-12-22 [PMID: 36465110]
L Rytel, S Gonkowski The Influence of Bisphenol a on the Nitrergic Nervous Structures in the Domestic Porcine Uterus Int J Mol Sci, 2020-06-26;21(12):. 2020-06-26 [PMID: 32604714] (IHC, Porcine) IHC Porcine
K Palus, M Bulc, M Czajkowska, B Mici?ski, J Ca?ka Neurochemical characteristics of calbindin-like immunoreactive coeliac-cranial mesenteric ganglion complex (CCMG) neurons supplying the pre-pyloric region of the porcine stomach Tissue Cell, 2017-12-05;50(0):8-14. 2017-12-05 [PMID: 29429522] (ICC, Porcine) ICC Porcine
Janusz Najdzion Cocaine- and amphetamine-regulated transcript peptide and calcium binding proteins immunoreactivity in the superficial layers of the superior colliculus in the guinea pig: Implications for visual sensory processing J. Chem. Neuroanat, 2016-11-28;79(0):66-79. 2016-11-28 [PMID: 27908658] (IHC, Guinea Pig) IHC Guinea Pig
Malgorzata Radzimirska, Krystyna Bogus-Nowakowska, Tadeusz Kuder, Anna Robak Distribution of cocaine- and amphetamine-regulated transcript (CART), neuropeptide Y (NPY) and galanin (GAL) in the pterygopalatine ganglion of the domestic duck (Anas platyrhynchos f. domestica) Folia Histochemica et Cytobiologica 2015-09-01 [PMID: 27044589]
Isensee J, Wenzel C, Buschow R, Weissmann R, Kuss A, Hucho T Subgroup-elimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit. PLoS ONE, 2014-12-31;9(12):e115731. 2014-12-31 [PMID: 25551770] (IHC-Fr, Rat) IHC-Fr Rat
Romke Bron, Lei Yin, Domenico Russo, John B. Furness Expression of the ghrelin receptor gene in neurons of the medulla oblongata of the rat Journal of Comparative Neurology 2013-08-15 [PMID: 23348715]
Gonsalvez DG, Kerman IA, McAllen RM Chemical coding for cardiovascular sympathetic preganglionic neurons in rats. J. Neurosci., 2010-09-01;30(35):11781-91. 2010-09-01 [PMID: 20810898] (IHC-Fr, Rat) IHC-Fr Rat

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Bioinformatics

Gene Symbol CARTPT
Uniprot